User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/19: Difference between revisions

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===Procedures: Gold Nanoparticle Synthesis by Sodium Citrate Reduction Methods===
===Procedures: Gold Nanoparticle Synthesis by Sodium Citrate Reduction Methods===
Desired gold nanoparticle size: approximately 10nm.
Desired gold nanoparticle size: approximately 10nm.
====First Possibility====
====First Procedure====
Adapted from [http://pubs.acs.org/doi/full/10.1021/la034348u Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles].<br.>
Adapted from [http://pubs.acs.org/doi/full/10.1021/la034348u Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles].<br.>
*Boil 50mL MilliQ water in a clean beaker
*Boil 50mL MilliQ water in a clean beaker
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*Replace water lost to evaporation during boiling
*Replace water lost to evaporation during boiling
*If a higher concentration is desired, centrifuge  at 14 000 rpm and resuspended in MilliQ water
*If a higher concentration is desired, centrifuge  at 14 000 rpm and resuspended in MilliQ water
====Second Possibility====
====Second Procedure====
Adapted from [http://pubs.acs.org/doi/full/10.1021/ac0503198 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification].<br.>
Adapted from [http://pubs.acs.org/doi/full/10.1021/ac0503198 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification].<br.>
*Bring 100 mL of solution containing 0.01 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to reflux  
*Bring 100 mL of solution containing 0.01 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to reflux  

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July 18, 2013

Procedures: Gold Nanoparticle Synthesis by Sodium Citrate Reduction Methods

Desired gold nanoparticle size: approximately 10nm.

First Procedure

Adapted from Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles.<br.>

  • Boil 50mL MilliQ water in a clean beaker
  • Add 0.5 mL of HAuCl4 solution (1% w/v)
  • Follow with 1.75 mL of sodium citrate solution (1% w/v)
  • Boil for about 10 minutes until solution color changes to bright red
  • Replace water lost to evaporation during boiling
  • If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water

Second Procedure

Adapted from 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification.<br.>

  • Bring 100 mL of solution containing 0.01 g of HAuCl4·3H2O to reflux
  • Add 3 mL of 1% sodium citrate solution while stirring
  • Boil solution for another 40 minutes
  • Cool to room temperature

Original Procedure

From Allison Alix on 05/10/2013.<br.>

  • Stir and heat to boiling 20mL of 1mM HAuCl4
  • Add 2mL 1% sodium citrate
  • Observe a purple to red color change
  • Determine size and concentration of AuNP using UV-Vis


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