User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/19: Difference between revisions
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*Replace water lost to evaporation during boiling | *Replace water lost to evaporation during boiling | ||
*If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water | *If a higher concentration is desired, centrifuge at 14 000 rpm and resuspended in MilliQ water | ||
=====Absorbance Data===== | |||
[[Image:2013_0719_new_AuNP_procedure_1.PNG]] | [[Image:2013_0719_new_AuNP_procedure_1.PNG]] | ||
====Second Procedure==== | ====Second Procedure==== | ||
Adapted from [http://pubs.acs.org/doi/full/10.1021/ac0503198 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification].<br.> | Adapted from [http://pubs.acs.org/doi/full/10.1021/ac0503198 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification].<br.> | ||
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*Boil solution for another 40 minutes | *Boil solution for another 40 minutes | ||
*Cool to room temperature | *Cool to room temperature | ||
=====Absorbance Data===== | |||
[[Image:2013_0719_new_AuNP_procedure_2.PNG]] | |||
====Original Procedure==== | ====Original Procedure==== | ||
From Allison Alix on [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/05/10 05/10/2013].<br.> | From Allison Alix on [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/05/10 05/10/2013].<br.> | ||
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*Observe a purple to red color change | *Observe a purple to red color change | ||
*Determine size and concentration of AuNP using UV-Vis | *Determine size and concentration of AuNP using UV-Vis | ||
=====Absorbance Data====== | |||
Solution too concentrated; will be diluted and measurements taken again tomorrow. | |||
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Revision as of 11:49, 19 July 2013
AuNP Synthesis | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 18, 2013Procedures: Gold Nanoparticle Synthesis by Sodium Citrate Reduction MethodsDesired gold nanoparticle size: approximately 10nm. First ProcedureAdapted from Nonspecific and Thiol-Specific Binding of DNA to Gold Nanoparticles.<br.>
Absorbance DataSecond ProcedureAdapted from 4-(Dimethylamino)butyric Acid Labeling for Electrochemiluminescence Detection of Biological Substances by Increasing Sensitivity with Gold Nanoparticle Amplification.<br.>
Absorbance DataOriginal ProcedureFrom Allison Alix on 05/10/2013.<br.>
Absorbance Data=Solution too concentrated; will be diluted and measurements taken again tomorrow. |