User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/23: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MHA Day Two Procedure, Absorbance Measurements, Meeting Notes
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:16px;"> MHA Day Two Procedure, Absorbance Measurements, Meeting Notes</span>
</span>
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*Day Two:
*Day Two:
**Prepare the following solutions:
**Prepare the following solutions:
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
#1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
#*Stir solutions for one hour to allow reaction to proceed
#*Stir solutions for one hour to allow reaction to proceed
#*Centrifuge solutions at 4000 rpm for one hour
#*Centrifuge solutions at 4000 rpm for one hour
#*Re-suspend particles in 140uL PBS (per solution)
#*Re-suspend particles in 140uL PBS (per solution)
#**Dilute each solution 10 and 100 times
#**Dilute each solution 10 times
===Absorbance Measurements===
===Notes from Meeting===
===Notes from Meeting===
 
*Don't compare absorbance spectra
*Peak shift > color change > aggregation indication?
*Time for silica-coating procedure?
*Could we use larger AuNP@SiO2? since we know we can make them
*Igor Pro: SiO2 thickness
**Look up programs?




Revision as of 12:15, 24 July 2013

MHA Day Two Procedure, Absorbance Measurements, Meeting Notes <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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July 23, 2013

Procedure:Silica-coating AuNPs by MHA, Day Two Round 2

  • Day Two:
    • Prepare the following solutions:
  1. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
  5. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
  6. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
  7. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
  8. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
    • Stir solutions for one hour to allow reaction to proceed
    • Centrifuge solutions at 4000 rpm for one hour
    • Re-suspend particles in 140uL PBS (per solution)
      • Dilute each solution 10 times

Notes from Meeting

  • Don't compare absorbance spectra
  • Peak shift > color change > aggregation indication?
  • Time for silica-coating procedure?
  • Could we use larger AuNP@SiO2? since we know we can make them
  • Igor Pro: SiO2 thickness
    • Look up programs?



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