User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/23: Difference between revisions

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#*Centrifuge solutions at 4000 rpm for one hour
#*Centrifuge solutions at 4000 rpm for one hour
#*Re-suspend particles in 140uL PBS (per solution)
#*Re-suspend particles in 140uL PBS (per solution)
#**Dilute each solution 10 and 100 times
#**Dilute each solution 10 times
*Samples of the AuNPs with MHA dissolved in both isopropanol and PBS will be centrifuged and redispersed, like all the other samples, so abosrbance measurements taken of them can serve as a comparison
 
===Absorbance Measurements===
===Notes from Meeting===
===Notes from Meeting===
 
*Don't compare absorbance spectra
*Peak shift > color change > aggregation indication?
*Time for silica-coating procedure?
*Could we use larger AuNP@SiO2? since we know we can make them
*Igor Pro: SiO2 thickness
**Look up programs?




Revision as of 12:15, 24 July 2013

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July 23, 2013

Procedure:Silica-coating AuNPs by MHA, Day Two Round 2

  • Day Two:
    • Prepare the following solutions:
  1. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
  5. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
  6. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
  7. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
  8. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
    • Stir solutions for one hour to allow reaction to proceed
    • Centrifuge solutions at 4000 rpm for one hour
    • Re-suspend particles in 140uL PBS (per solution)
      • Dilute each solution 10 times

Notes from Meeting

  • Don't compare absorbance spectra
  • Peak shift > color change > aggregation indication?
  • Time for silica-coating procedure?
  • Could we use larger AuNP@SiO2? since we know we can make them
  • Igor Pro: SiO2 thickness
    • Look up programs?



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