User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/23

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(July 23, 2013)
(Procedure:Silica-coating AuNPs by MHA, Day Two Round 2)
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#*Centrifuge solutions at 4000 rpm for one hour
#*Centrifuge solutions at 4000 rpm for one hour
#*Re-suspend particles in 140uL PBS (per solution)
#*Re-suspend particles in 140uL PBS (per solution)
-
#**Dilute each solution 10 and 100 times
+
#**Dilute each solution 10 times
 +
 
===Absorbance Measurements===
===Absorbance Measurements===
===Notes from Meeting===
===Notes from Meeting===

Revision as of 13:44, 23 July 2013

MHA Day Two Procedure, Absorbance Measurements, Meeting Notes Main project page
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July 23, 2013

Procedure:Silica-coating AuNPs by MHA, Day Two Round 2

  • Day Two:
    • Prepare the following solutions:
  1. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
  5. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
  6. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
  7. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
  8. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
    • Stir solutions for one hour to allow reaction to proceed
    • Centrifuge solutions at 4000 rpm for one hour
    • Re-suspend particles in 140uL PBS (per solution)
      • Dilute each solution 10 times

Absorbance Measurements

Notes from Meeting


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