User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/23

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(Procedure:Silica-coating AuNPs by MHA, Day Two Round 2)
Current revision (15:15, 24 July 2013) (view source)
(July 23, 2013)
 
Line 22: Line 22:
#*Re-suspend particles in 140uL PBS (per solution)
#*Re-suspend particles in 140uL PBS (per solution)
#**Dilute each solution 10 times
#**Dilute each solution 10 times
-
 
-
===Absorbance Measurements===
 
===Notes from Meeting===
===Notes from Meeting===
-
 
+
*Don't compare absorbance spectra
 +
*Peak shift > color change > aggregation indication?
 +
*Time for silica-coating procedure?
 +
*Could we use larger AuNP@SiO2? since we know we can make them
 +
*Igor Pro: SiO2 thickness
 +
**Look up programs?

Current revision

MHA Day Two Procedure, Absorbance Measurements, Meeting Notes Main project page
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July 23, 2013

Procedure:Silica-coating AuNPs by MHA, Day Two Round 2

  • Day Two:
    • Prepare the following solutions:
  1. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/4μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/3μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/2μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 9.65nm AuNP&MHA, isopropanol/1μL TES/0.125mL NH4OH/5mL propanol
  5. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
  6. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
  7. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
  8. 1mL 9.4nM, 9.65nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
    • Stir solutions for one hour to allow reaction to proceed
    • Centrifuge solutions at 4000 rpm for one hour
    • Re-suspend particles in 140uL PBS (per solution)
      • Dilute each solution 10 times

Notes from Meeting

  • Don't compare absorbance spectra
  • Peak shift > color change > aggregation indication?
  • Time for silica-coating procedure?
  • Could we use larger AuNP@SiO2? since we know we can make them
  • Igor Pro: SiO2 thickness
    • Look up programs?



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