User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/24: Difference between revisions
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====Notes and Observations==== | ====Notes and Observations==== | ||
MHA and AuNP solution was heated just enough to allow MHA to dissolve. 2uL and 4uL samples appeared much clearer than other samples which had visible particles floating around in solution (prior to centrifugation). It looked as if the nanoparticles had stuck to the bottom of these two vials. After centrifugation, 2uL and 4uL samples produced noticeably less particles and were visibly a lighter color than the other samples. All samples seemed to be more purple in color than the pink characteristic of gold nanoparticles. This may be similar to what happened with the samples we made and took TEM images of last week, and could be indicative of aggregation. | MHA and AuNP solution was heated just enough to allow MHA to dissolve. 2uL and 4uL samples appeared much clearer than other samples which had visible particles floating around in solution (prior to centrifugation). It looked as if the nanoparticles had stuck to the bottom of these two vials. After centrifugation, 2uL and 4uL samples produced noticeably less particles and were visibly a lighter color than the other samples. All samples seemed to be more purple in color than the pink characteristic of gold nanoparticles. This may be similar to what happened with the samples we made and took TEM images of last week, and could be indicative of aggregation. | ||
===Absorbance Data=== | |||
[[Image:2013_0724_AuNPs@SiO2.PNG]] | |||
===For NIST Tomorrow=== | ===For NIST Tomorrow=== | ||
*10nm AuNPs from 06/17/2013, 9.4nM | *10nm AuNPs from 06/17/2013, 9.4nM | ||
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*AuNPs in MHA from 07/23/2013 (2 samples) | *AuNPs in MHA from 07/23/2013 (2 samples) | ||
*AuNPs@SiO2 from 07/23/2013 (8 samples) | *AuNPs@SiO2 from 07/23/2013 (8 samples) | ||
*AuNPs@SiO2 from | *AuNPs@SiO2 from 07/24/2013 (5 samples) | ||
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Revision as of 11:52, 24 July 2013
 MHA Round Three
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July 24, 2013Procedure:Silica-coating AuNPs by MHA, Day Two Round 3
Notes and ObservationsMHA and AuNP solution was heated just enough to allow MHA to dissolve. 2uL and 4uL samples appeared much clearer than other samples which had visible particles floating around in solution (prior to centrifugation). It looked as if the nanoparticles had stuck to the bottom of these two vials. After centrifugation, 2uL and 4uL samples produced noticeably less particles and were visibly a lighter color than the other samples. All samples seemed to be more purple in color than the pink characteristic of gold nanoparticles. This may be similar to what happened with the samples we made and took TEM images of last week, and could be indicative of aggregation. Absorbance Data
For NIST Tomorrow
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