User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/24

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MHA Round Three</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==July 24, 2013==
==July 24, 2013==
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===Procedure:Silica-coating AuNPs by MHA, Day Two Round 3===
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*Using 9.4nM, 10nm AuNPs in PBS from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/19 06/19/2013] (used in first silica-coating procedure conducted on 06/20/2013)
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*Day Two:
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**Prepare the following solutions:
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#1mL 9.4nM, 10nm AuNP&MHA, PBS/5μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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#1mL 9.4nM, 10nm AuNP&MHA, PBS/4μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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#1mL 9.4nM, 10nm AuNP&MHA, PBS/3μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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#1mL 9.4nM, 10nm AuNP&MHA, PBS/2μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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#1mL 9.4nM, 10nm AuNP&MHA, PBS/1μL TES/0.125mL NH<sub>4</sub>OH/5mL propanol
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#*Stir solutions for one hour to allow reaction to proceed
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#*Centrifuge solutions at 4000 rpm for one hour
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#*Remove supernatants and discard
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#*Re-suspend particles in 140uL PBS (per solution)
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#**Dilute each solution 10 times to run absorbance measurements
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Revision as of 09:05, 24 July 2013

MHA Round Three Main project page
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July 24, 2013

Procedure:Silica-coating AuNPs by MHA, Day Two Round 3

  • Using 9.4nM, 10nm AuNPs in PBS from 06/19/2013 (used in first silica-coating procedure conducted on 06/20/2013)
  • Day Two:
    • Prepare the following solutions:
  1. 1mL 9.4nM, 10nm AuNP&MHA, PBS/5μL TES/0.125mL NH4OH/5mL propanol
  2. 1mL 9.4nM, 10nm AuNP&MHA, PBS/4μL TES/0.125mL NH4OH/5mL propanol
  3. 1mL 9.4nM, 10nm AuNP&MHA, PBS/3μL TES/0.125mL NH4OH/5mL propanol
  4. 1mL 9.4nM, 10nm AuNP&MHA, PBS/2μL TES/0.125mL NH4OH/5mL propanol
  5. 1mL 9.4nM, 10nm AuNP&MHA, PBS/1μL TES/0.125mL NH4OH/5mL propanol
    • Stir solutions for one hour to allow reaction to proceed
    • Centrifuge solutions at 4000 rpm for one hour
    • Remove supernatants and discard
    • Re-suspend particles in 140uL PBS (per solution)
      • Dilute each solution 10 times to run absorbance measurements


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