User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/25: Difference between revisions
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==July 25, 2013== | ==July 25, 2013== | ||
===Procedure Update: MHA Silica-Coating=== | ===Procedure Update: MHA Silica-Coating=== | ||
According to [http://link.springer.com/content/pdf/10.1007%2Fs11051-012-0758-z.pdf Aggregation and adhesion of gold nanoparticles in phosphate buffer saline], PBS and prolonged sonication actually cause aggregation in gold nanoparticle solutions: "results showed an optimized sonication time of 10 min to reach equilibrium for plain gold nanoparticles in water." Instead of using PBS to dilute all components, filtered MilliQ water will be used to dilute nanoparticles as well as MHA, as the organic solvent in solution prior to the actual silica-coating may have also been affecting observed the gold nanoparticle aggregation. | According to [http://link.springer.com/content/pdf/10.1007%2Fs11051-012-0758-z.pdf Aggregation and adhesion of gold nanoparticles in phosphate buffer saline], PBS and prolonged sonication actually cause aggregation in gold nanoparticle solutions: "results showed an optimized sonication time of 10 min to reach equilibrium for plain gold nanoparticles in water." Instead of using PBS to dilute all components, filtered MilliQ water will be used to dilute nanoparticles as well as MHA, as the organic solvent in solution prior to the actual silica-coating may have also been affecting observed the gold nanoparticle aggregation. Filtered MilliQ water will be brought up to room temperature as we've noticed cold solutions (whether water or PBS) have seemed to induce aggregation as well. | ||
*Originally detailed on [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] | *Originally detailed on [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] | ||
*Last updated on [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] | *Last updated on [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/12#Procedure_Update:_Silica-coating_AuNPs_by_MHA 07/12/2013] |
Revision as of 07:46, 25 July 2013
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July 25, 2013Procedure Update: MHA Silica-CoatingAccording to Aggregation and adhesion of gold nanoparticles in phosphate buffer saline, PBS and prolonged sonication actually cause aggregation in gold nanoparticle solutions: "results showed an optimized sonication time of 10 min to reach equilibrium for plain gold nanoparticles in water." Instead of using PBS to dilute all components, filtered MilliQ water will be used to dilute nanoparticles as well as MHA, as the organic solvent in solution prior to the actual silica-coating may have also been affecting observed the gold nanoparticle aggregation. Filtered MilliQ water will be brought up to room temperature as we've noticed cold solutions (whether water or PBS) have seemed to induce aggregation as well.
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