User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/29: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Absorbance Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==July 29, 2013== | ||
* | ===Absorbance Data: AuNPs@SiO<sub>2</sub>=== | ||
[[Image:2013_0729_AuNPs@SiO2.PNG]]<br.> | |||
Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents). | |||
====Notes==== | |||
Spectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature. The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation. Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution. | |||
===For NIST Tomorrow=== | |||
*9.65nm, 23.5nM AuNPs | |||
*9.65nm, 4.7nM AuNPs with MHA (in water) | |||
*AuNPs@SiO<sub>2</sub>- MHA in water | |||
**5uL | |||
**4uL | |||
**3uL | |||
**2uL | |||
**1uL | |||
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Latest revision as of 23:08, 26 September 2017
Absorbance Measurements | Main project page Previous entry Next entry |
July 29, 2013Absorbance Data: AuNPs@SiO2<br.> Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents). NotesSpectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature. The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation. Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution. For NIST Tomorrow
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