User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/29

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Current revision (12:33, 29 July 2013) (view source)
(July 29, 2013)
 
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[[Image:2013_0729_AuNPs@SiO2.PNG]]<br.>
[[Image:2013_0729_AuNPs@SiO2.PNG]]<br.>
Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents).
Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents).
 +
====Notes====
 +
Spectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature.  The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation.  Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution.
===For NIST Tomorrow===
===For NIST Tomorrow===
*9.65nm, 23.5nM AuNPs
*9.65nm, 23.5nM AuNPs
*9.65nm, 4.7nM AuNPs with MHA (in water)
*9.65nm, 4.7nM AuNPs with MHA (in water)
-
*AuNPs@SiO<sub>2</sub>
+
*AuNPs@SiO<sub>2</sub>- MHA in water
**5uL
**5uL
**4uL
**4uL

Current revision

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July 29, 2013

Absorbance Data: AuNPs@SiO2

Image:2013_0729_AuNPs@SiO2.PNG
Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents).

Notes

Spectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature. The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation. Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution.

For NIST Tomorrow

  • 9.65nm, 23.5nM AuNPs
  • 9.65nm, 4.7nM AuNPs with MHA (in water)
  • AuNPs@SiO2- MHA in water
    • 5uL
    • 4uL
    • 3uL
    • 2uL
    • 1uL


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