User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/18

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(Procedures)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ZnPPIX Samples</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==September 18, 2013==
==September 18, 2013==
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*Remake 06/05/2013 ZnPPIX samples
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*Take UV-vis and fluorescence measurements of remade samples
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===Procedures===
===Procedures===
<u>Remake 06/05/13 Replicate Samples</u><br.>
<u>Remake 06/05/13 Replicate Samples</u><br.>
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*Calculations from 09/11/2013 used again
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*PBS used in place of water
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*Solutions were made, vortexed, and allowed to react for 10 minutes
===Data===
===Data===
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[[Image:2013_0918_ZnPPIX_abs_fluor_data.PNG]]<br.>
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Excitation wavelength: 440nm<br.>
===Notes===
===Notes===
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Again, no fluorescence was observed. I wanted to make the samples over again with the nanoparticles previously used (when fluorescence was observed), but I was unable to find them and they may have been used up.
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Current revision

ZnPPIX Samples Main project page
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September 18, 2013

  • Remake 06/05/2013 ZnPPIX samples
  • Take UV-vis and fluorescence measurements of remade samples

Procedures

Remake 06/05/13 Replicate Samples

  • Calculations from 09/11/2013 used again
  • PBS used in place of water
  • Solutions were made, vortexed, and allowed to react for 10 minutes

Data

Image:2013_0918_ZnPPIX_abs_fluor_data.PNG
Excitation wavelength: 440nm

Notes

Again, no fluorescence was observed. I wanted to make the samples over again with the nanoparticles previously used (when fluorescence was observed), but I was unable to find them and they may have been used up.


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