User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/25: Difference between revisions

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*Check DNA with ThT
*Check DNA with ThT
===Procedures===
===Procedures===
#Parse out 53.1uM DNA into 10uL aliquots to prevent as much freeze/thaw of overall sample as possible


===Data===
===Data===

Revision as of 06:06, 25 September 2013

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September 25, 2013

Notes

Since I have been unable to replicate the procedure done previously in which I observed signal with the ZnPPIX, there are a couple of procedures I want to attempt in order to identify the problem:<br.>

  • ZnPPIX absorbance spectra to find the molar absorptivity, ε
    • Check concentration (as well as DNA)
  • Get new DNA samples, parse them out to smaller aliquots
    • Switch to 4 and 8 strand DNA, adjusting [ZnPPIX] as needed
  • Ask Dr Hartings about ZnPPIX storage/time?
  • Look at Ellen/Eleni's ZnPPIX absorbance spectrum
  • Check DNA with ThT

Procedures

  1. Parse out 53.1uM DNA into 10uL aliquots to prevent as much freeze/thaw of overall sample as possible


Data


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