User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/02: Difference between revisions
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== | ==October 2, 2013== | ||
===Procedures=== | ===Procedures=== | ||
*Absorbance measurements and Beer's law calculations for samples from 10/25/2013 | |||
*Sample preparation based on calculated concentrations | |||
===Data=== | ===Data=== | ||
=====Absorbance Measurements from 10/25/2013===== | |||
[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.> | |||
Beer's Law calculations:<br.> | |||
[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]] | |||
=====Absorbance Measurements, Today===== | |||
I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using. | |||
<br.> | |||
[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.> | |||
Beer's Law calculations:<br.> | |||
[[Image:2013_1002_ZnPPIX_calcs.PNG]] | |||
=====Samples Prepared===== | |||
"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples | |||
*2.47uM ZnPPIX | |||
*2.47uM ZnPPIX/1.11uM DNA-SH | |||
*2.47uM ZnPPIX/1.11uM DNA (4) | |||
*2.47uM ZnPPIX/1.11uM DNA (8) | |||
*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs | |||
*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs | |||
*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs | |||
*2.47uM ZnPPIX/11.75nM AuNPs | |||
===Notes=== | ===Notes=== | ||
*Run today's samples for absorbance and fluorescence | |||
*Ask Dr Hartings for more ZnPPIX | |||
Revision as of 08:35, 2 October 2013
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October 2, 2013Procedures
DataAbsorbance Measurements from 10/25/2013<br.> Beer's Law calculations:<br.> Absorbance Measurements, TodayI decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using. <br.> <br.> Beer's Law calculations:<br.> Samples Prepared"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
Notes
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