User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/02

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Current revision (10:35, 2 October 2013) (view source)
 
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==Date==
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==October 2, 2013==
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===Procedures===
===Procedures===
 +
*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
 +
*Sample preparation based on calculated concentrations
===Data===
===Data===
 +
=====Absorbance Measurements from 10/25/2013=====
 +
[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
 +
Beer's Law calculations:<br.>
 +
[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]
 +
=====Absorbance Measurements, Today=====
 +
I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
 +
<br.>
 +
[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
 +
Beer's Law calculations:<br.>
 +
[[Image:2013_1002_ZnPPIX_calcs.PNG]]
 +
 +
=====Samples Prepared=====
 +
"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
 +
*2.47uM ZnPPIX
 +
*2.47uM ZnPPIX/1.11uM DNA-SH
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*2.47uM ZnPPIX/1.11uM DNA (4)
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*2.47uM ZnPPIX/1.11uM DNA (8)
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*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
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*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
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*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
 +
*2.47uM ZnPPIX/11.75nM AuNPs
===Notes===
===Notes===
 +
*Run today's samples for absorbance and fluorescence
 +
*Ask Dr Hartings for more ZnPPIX
 +

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October 2, 2013

Procedures

  • Absorbance measurements and Beer's law calculations for samples from 10/25/2013
  • Sample preparation based on calculated concentrations

Data

Absorbance Measurements from 10/25/2013

Image:2013_1002_ZnPPIX_from_last_week.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG

Absorbance Measurements, Today

I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
Image:2013_1002_ZnPPIX_for_calcs.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_calcs.PNG

Samples Prepared

"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples

  • 2.47uM ZnPPIX
  • 2.47uM ZnPPIX/1.11uM DNA-SH
  • 2.47uM ZnPPIX/1.11uM DNA (4)
  • 2.47uM ZnPPIX/1.11uM DNA (8)
  • 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
  • 2.47uM ZnPPIX/11.75nM AuNPs

Notes

  • Run today's samples for absorbance and fluorescence
  • Ask Dr Hartings for more ZnPPIX



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