User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/10/02 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
 
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Date==
==October 2, 2013==
 
===Procedures===
===Procedures===
*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
*Sample preparation based on calculated concentrations


===Data===
===Data===
=====Absorbance Measurements from 10/25/2013=====
[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
Beer's Law calculations:<br.>
[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]


=====Absorbance Measurements, Today=====
I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
<br.>
[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
Beer's Law calculations:<br.>
[[Image:2013_1002_ZnPPIX_calcs.PNG]]
=====Samples Prepared=====
"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
*2.47uM ZnPPIX
*2.47uM ZnPPIX/1.11uM DNA-SH
*2.47uM ZnPPIX/1.11uM DNA (4)
*2.47uM ZnPPIX/1.11uM DNA (8)
*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
*2.47uM ZnPPIX/11.75nM AuNPs


===Notes===
===Notes===
*Run today's samples for absorbance and fluorescence
*Ask Dr Hartings for more ZnPPIX




Revision as of 08:35, 2 October 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 2, 2013

Procedures

  • Absorbance measurements and Beer's law calculations for samples from 10/25/2013
  • Sample preparation based on calculated concentrations

Data

Absorbance Measurements from 10/25/2013

<br.> Beer's Law calculations:<br.>

Absorbance Measurements, Today

I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using. <br.> <br.> Beer's Law calculations:<br.>

Samples Prepared

"20uM" and "50uM" ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples

  • 2.47uM ZnPPIX
  • 2.47uM ZnPPIX/1.11uM DNA-SH
  • 2.47uM ZnPPIX/1.11uM DNA (4)
  • 2.47uM ZnPPIX/1.11uM DNA (8)
  • 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
  • 2.47uM ZnPPIX/11.75nM AuNPs

Notes

  • Run today's samples for absorbance and fluorescence
  • Ask Dr Hartings for more ZnPPIX



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/10/02&oldid=734336"