User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ZnPPIX Synthesis</span> | ||
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==October 9, 2013== | |||
===Procedures=== | ===Procedures=== | ||
* | *Synthesize new ZnPPIX with Dr Hartings | ||
* | *Procedure adapted from "On the preparation of metalloporphyrins" | ||
**0.5g tetraphenylporphyrin refluxed in 50mL DMF | |||
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete | |||
***completion determination by unchanged peaks in UV-vis measurements | |||
**50mL distilled water added, solution chilled and then filtered | |||
***the filtrate was filtered over again and added to main filtered sample | |||
===Data=== | ===Data=== | ||
Absorbance Measurements | |||
*Used to determine reaction completion (based on the change in the peaks) | |||
[[Image:2012 1009 ZnPPPIX abs.PNG]] | |||
No visible change in peaks between 'ZnPPIX#2 100x' (dark blue line- the second sample of refluxing ZnPPIX taken) and 'ZnPPIX after' (light blue line- the final sample taken when reflux was stopped), used to determine that the reaction was complete.<br.> | |||
Peaks consistent with the literature: largest peak at ~500nm, next largest at ~600nm, smallest peak at ~550nm. | |||
[[Image: | |||
===Notes=== | ===Notes=== | ||
Next time: | |||
* | *Dissolve filtered ZnPPIX in DMF | ||
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Revision as of 08:31, 16 October 2013
ZnPPIX Synthesis | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
October 9, 2013Procedures
DataAbsorbance Measurements
No visible change in peaks between 'ZnPPIX#2 100x' (dark blue line- the second sample of refluxing ZnPPIX taken) and 'ZnPPIX after' (light blue line- the final sample taken when reflux was stopped), used to determine that the reaction was complete.<br.> Peaks consistent with the literature: largest peak at ~500nm, next largest at ~600nm, smallest peak at ~550nm. NotesNext time:
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