User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09

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(October 2, 2013)
(Samples Prepared)
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=====Samples Prepared=====
=====Samples Prepared=====
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20uM and 50uM ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples
*2.47uM ZnPPIX
*2.47uM ZnPPIX
*2.47uM ZnPPIX/1.11uM DNA-SH
*2.47uM ZnPPIX/1.11uM DNA-SH

Revision as of 10:24, 2 October 2013

UV-vis Measurements & Sample Preparation Main project page
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October 2, 2013

Procedures

  • Absorbance measurements and Beer's law calculations for samples from 10/25/2013
  • Sample preparation based on calculated concentrations

Data

Absorbance Measurements from 10/25/2013

Image:2013_1002_ZnPPIX_from_last_week.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG

Absorbance Measurements, Today

I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
Image:2013_1002_ZnPPIX_for_calcs.PNG
Beer's Law calculations:
Image:2013_1002_ZnPPIX_calcs.PNG

Samples Prepared

20uM and 50uM ZnPPIX dilutions made today, fresh aliquots of all three DNA strands, and 23.5nM AuNps were used to prepare these samples

  • 2.47uM ZnPPIX
  • 2.47uM ZnPPIX/1.11uM DNA-SH
  • 2.47uM ZnPPIX/1.11uM DNA (4)
  • 2.47uM ZnPPIX/1.11uM DNA (8)
  • 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
  • 2.47uM ZnPPIX/11.75nM AuNPs

Notes

  • Run today's samples for absorbance and fluorescence
  • Ask Dr Hartings for more ZnPPIX


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