User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/16: Difference between revisions

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==October 16, 2013==
==October 16, 2013==
===Procedures===
===Procedures===
*Dissolve ZnPPIX made 10/09/13 in DMF
*Dissolve ZnPPIX made [10/09/13 http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/10/09] in DMF
*Filter ZnPPIX solution to remove any more zinc or porphyrin impurities
*Filter ZnPPIX solution to remove any more zinc or porphyrin impurities
*Allow to dry overnight
*Allow to dry overnight

Revision as of 08:13, 16 October 2013

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October 16, 2013

Procedures

Data

  • 0.0146g ZnPPIX dissolved in 13mL DMF
  • MW ZnPPIX: 626.032g/mol

(0.0146g)×(626.032g/mol)×(1/0.013L)≈1.793mM<br.>

  • ε412 ZnPPIX in phosphate buffer: 87.4mM/cm
    • (from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE")

Notes

Next week:

  • Re-dissolve ZnPPIX in DMF
  • Prepared several dilutions (100x, 1000x, 10000x?)
  • Take UV-vis measurements
  • Use Beer's law to determine concentration


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