User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/06

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*Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
*Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
*From here: redissolve purified ZnPPIX in DMF, calculated concentration, made 10x (and 100x?) dilution to run UV-vis measurements & determine concentration using Beer's law, do calculations & make samples with new ZnPPIX, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra
*From here: redissolve purified ZnPPIX in DMF, calculated concentration, made 10x (and 100x?) dilution to run UV-vis measurements & determine concentration using Beer's law, do calculations & make samples with new ZnPPIX, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra
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===Additional Notes===
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*ZnPPIX solution never crystallized, so I was unable to filter it.
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Current revision

ZnPPIX Filtration Main project page
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November 6, 2013

Procedures

  • Further purification of ZnPPIX by procedure from Dr Hartings in "Purification of Laboratory Chemicals" By Wilfred L.F. Armarego and Christina Li Lin Chai
    • Hemin (ferriproptoporphyrin IX chloride)procedure used, substituting ZnPPIX for heme- calculations done to determine 1:1 molar ratio between ZnPPIX and acetic acid (AcOH)

Notes

  • Initial ZnPPIX mixture made and filtration performed
  • Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
  • From here: redissolve purified ZnPPIX in DMF, calculated concentration, made 10x (and 100x?) dilution to run UV-vis measurements & determine concentration using Beer's law, do calculations & make samples with new ZnPPIX, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra

Additional Notes

  • ZnPPIX solution never crystallized, so I was unable to filter it.


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