User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/06

From OpenWetWare
Jump to navigationJump to search
ZnPPIX Filtration <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

November 6, 2013

Procedures

  • Further purification of ZnPPIX by procedure from Dr Hartings in "Purification of Laboratory Chemicals" By Wilfred L.F. Armarego and Christina Li Lin Chai
    • Hemin (ferriproptoporphyrin IX chloride)procedure used, substituting ZnPPIX for heme- calculations done to determine 1:1 molar ratio between ZnPPIX and acetic acid (AcOH)

Notes

  • Initial ZnPPIX mixture made and filtration performed
  • Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
  • Next week: redissolve purified ZnPPIX in DMF, made samples, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/11/06&oldid=753961"