User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/13

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Current revision (11:20, 13 November 2013) (view source)
 
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===Data===
===Data===
[[Image:2013_1113_abs_ZnPPIX.PNG]]<br.>
[[Image:2013_1113_abs_ZnPPIX.PNG]]<br.>
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+
Beer's Law calculations:
 +
[[Image:2013_1113_ZnPPIX_beer's_law_calcs.PNG]]
===Notes===
===Notes===
*In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete
*In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete

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November 13, 2013

Procedures

  • Synthesis of ZnPPIX
    • 1:1 molar ratio of Zinc nitrate hexahydrate to Dr Miller's porphyrin
    • Dissolve 0.0054g Zn nitrate and 0.0144g porphyrin in 5mL DMF
    • Take absorbance measurements every 20 minutes, making 100x dilutions
    • Continue heating until absorbance spectra stops changing (80 minutes)

Data

Image:2013_1113_abs_ZnPPIX.PNG
Beer's Law calculations: Image:2013_1113_ZnPPIX_beer's_law_calcs.PNG

Notes

  • In the third, fourth, and fifth samples, it is evident that the spectra stopped changing and the reaction was assumed to be complete


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