User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/20

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(Notes)
 
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===Notes===
===Notes===
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Increased fluoresence, though small, was observed for samples containing ZnPPIX, both 8 and 4 repeat DNA, and AuNPs.  I need to increase the concentration of ZnPPIX in the solutions containing the 8 repeat DNA, which will hopefully result in further increased fluoresence due to the formation of twice the amount of G-quadruplexes compared to the 4 repeat DNA.
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Increased fluoresence, though small, was observed for samples containing ZnPPIX, both 8 and 4 repeat DNA, and AuNPs.  I need to increase the concentration of ZnPPIX in the solutions containing the 8 repeat DNA, which will hopefully result in further increased fluoresence due to the formation of twice the amount of G-quadruplexes compared to the 4 repeat DNA.  I'm not quite sure why the ZnPPIX, ZnPPIX/4-DNA, and ZnPPIX/8-DNA samples' fluoresence did not quite reach the baseline that the ZnPPIX/x-DNA/AuNPs samples did.
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Current revision

ZnPPIX Sample Preparation Main project page
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November 20, 2013

Procedures

  • Do calculations and prepare samples with DNA and AuNPs
  • Run absorption measurements of those samples

Data

Image:2013_1120_spectra.PNG

Notes

Increased fluoresence, though small, was observed for samples containing ZnPPIX, both 8 and 4 repeat DNA, and AuNPs. I need to increase the concentration of ZnPPIX in the solutions containing the 8 repeat DNA, which will hopefully result in further increased fluoresence due to the formation of twice the amount of G-quadruplexes compared to the 4 repeat DNA. I'm not quite sure why the ZnPPIX, ZnPPIX/4-DNA, and ZnPPIX/8-DNA samples' fluoresence did not quite reach the baseline that the ZnPPIX/x-DNA/AuNPs samples did.


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