User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/06 - Revision history

Madeline Hartman: /* Entry title */

Madeline Hartman — Tue, 04 Dec 2012 17:00:56 GMT

Entry title

←Older revision		Revision as of 17:00, 4 December 2012
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	I ran two PCR reactions with the following content:		I ran two PCR reactions with the following content:

		+	Tube 1-
	# 40.6 μL distilled H2O		# 40.6 μL distilled H2O
	# 5.0 μL 10x cloned Pfu buffer		# 5.0 μL 10x cloned Pfu buffer
Line 16:		Line 17:
	# 1.0 μL Primer 2 (ng/μL)		# 1.0 μL Primer 2 (ng/μL)
	# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)		# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
		+
		+	and
		+
		+	Tube 2-
		+	# 20.3 μL distilled H2O
		+	# 5.0 μL 10x cloned Pfu buffer
		+	# 0.4 μL dNTPs (25 mM each)
		+	# 1.0 μL DNA template (100 ng/μL)
		+	# 1.0 μL Primer 1 (ng/μL)
		+	# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
		+	Tube 3-
		+	# 20.3 μL distilled H2O
		+	# 5.0 μL 10x cloned Pfu buffer
		+	# 0.4 μL dNTPs (25 mM each)
		+	# 1.0 μL DNA template (100 ng/μL)
		+	# 1.0 μL Primer 2 (ng/μL)
		+	# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
		+
		+	Tubes 2 and 3 were later combined.
		+
		+	Primer 1- t109c forward
		+
		+	Primer 2- t109c reverse
		+
		+	The three tubes were ran for the following cycles:
		+
		+	[[Image:MH 20121106Table of cycles.png\|300px]]
		+
		+
		+	After 5 cycles, tubes 2 and 3 were combined, and run the rest of the cycles together.
		+
		+	Tubes were left in thermocycler overnight at 4°C

Madeline Hartman: /* Entry title */

Madeline Hartman — Tue, 04 Dec 2012 16:46:02 GMT

Entry title

←Older revision		Revision as of 16:46, 4 December 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
	==Entry title==		==Entry title==
-	* Insert content ~~here~~...	+	I ran two PCR reactions with the following content:
		+
		+	# 40.6 μL distilled H2O
		+	# 5.0 μL 10x cloned Pfu buffer
		+	# 0.4 μL dNTPs (25 mM each)
		+	# 1.0 μL DNA template (100 ng/μL)
		+	# 1.0 μL Primer 1 (ng/μL)
		+	# 1.0 μL Primer 2 (ng/μL)
		+	# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)

Madeline Hartman: Autocreate 2012/11/06 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab

Madeline Hartman — Tue, 04 Dec 2012 16:39:12 GMT

Autocreate 2012/11/06 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab

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==Entry title==
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