User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/13: Difference between revisions

Latest revision as of 22:19, 26 September 2017

Project name

Gel electrophoresis was run on Tube 1 (original) and Tube 2+3 (forward/reverse). Forward/reverse was successful, original was now.

Gel

Lane 1- Marker

Lane 2- Original

Lane 3- Forward/reverse

Marker- 30 μL marker + 6 μL dye --> 6 μL added

Original 5 μL DNA + 1μL dye --> 6 μL added

Forward/reverse- 5μL DNA + 1μL dye --> 6 μL added

Gel was run for 45 minutes at 80 V with TAE buffer
Gel was removed from buffer and placed in ethidium bromide solution for 30 minutes while rocking
Gel was placed in rinse buffer for 15 minutes while rocking
Gel was viewed under UV light

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