User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/27 - Revision history

Madeline Hartman: /* Entry title */

2012-12-04T20:02:12Z

Entry title

←Older revision		Revision as of 20:02, 4 December 2012
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	# 200 μL of cells were plated and spread with a sterile spreader		# 200 μL of cells were plated and spread with a sterile spreader
	# The cells were incubated overnight at 37°C		# The cells were incubated overnight at 37°C
		+
		+	Cell transformation was unsuccessful

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Madeline Hartman: /* Entry title */

2012-12-04T19:59:16Z

Entry title

←Older revision		Revision as of 19:59, 4 December 2012
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	==Entry title==		==Entry title==
-	* Insert content here...	+	Cell Transformation was performed

		+	# BL21(DE3) cells thawed gently on ice
		+	# The competent cells were mixed. 100 μL cells were added to three separate 14-ml BD Falcon polypropylene round bottom tubes.
		+	# The three tubes were incubated on ice for 10 minutes, swirling gently ever 2 minutes
		+	# 5 μL, 10 μL, and 15 μL of ligated DNA were added to one of the three tubes, and swirled gently
		+	# The three tubes were incubated on ice for 30 minutes
		+	# Each of the three tubes was heat-pulsed for 45 seconds in a 42°C water bath
		+	# The three tubes were incubated on ice for 2 minutes
		+	# 0.9 ml of LB medium was added to each of the three tubes and the tubes were incubated at 37 °C for 1 hour while shaking at 225 rpm.
		+	# 200 μL of cells were plated and spread with a sterile spreader
		+	# The cells were incubated overnight at 37°C

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Madeline Hartman: Autocreate 2012/11/27 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab

2012-12-04T17:34:17Z

Autocreate 2012/11/27 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab

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==Entry title==
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