User:Madeline Hartman/Notebook/Dr. Hartings Lab/2014/02/20: Difference between revisions
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==Entry title== | ==Entry title== | ||
Ran a trial in OceanView for Absorbance readings. | |||
Run at 70°C set through Qpod | |||
#Run A Wizard | |||
#Select spectroscopy | |||
#JAZ is the correct hardware device. Click next | |||
#Select absorbance and click next | |||
#Select integration time as 1 ms | |||
#Select scans to average 10 | |||
#Click next | |||
#Open lamp. Click lightbulb to store reference spectrum. Click next | |||
#Close lamp. Click the lightbulb to store dark spectrum. Click finish | |||
#Open lamp. Click save graph to files. Configure | |||
#Save the first available scan every 1 minute | |||
#Stop after 2 hours | |||
#Save to directory in a dropbox folder - 02-20-H2O-70 | |||
#File format- Tab separated with headers | |||
#Create file name - mb_opd_acetate_h202_h20_70_ | |||
#Click apply | |||
# When ready to run, click play button, then click the save graph to files button a second time. | |||
Cuvette at start time contains- | |||
# 0.675 mL (2.8 mg OPD in 2.8 mL H2O) | |||
# 0.75 mL dilute H2O2 (0.1135 μL H2O2 and 0.8865 μL H2O) | |||
First reading taken after 45 seconds. | |||
0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Acetate buffer in 1 mL H2O) | |||
Steps were repeated at same temperature with 1 mg Mb/KCl/Citrate buffer in 1 mL H2O | |||
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Revision as of 09:16, 20 February 2014
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Entry titleRan a trial in OceanView for Absorbance readings. Run at 70°C set through Qpod
Cuvette at start time contains-
First reading taken after 45 seconds. 0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Acetate buffer in 1 mL H2O) Steps were repeated at same temperature with 1 mg Mb/KCl/Citrate buffer in 1 mL H2O
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