User:Madison Prieto/Notebook/Biology 210 at AU: Difference between revisions
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'''Purpose''' | '''Purpose''' | ||
The purpose of this lab is to use a Dichotomous Key to identify protists from our Hay Infusions and to | The purpose of this lab is to use a Dichotomous Key to identify protists from our Hay Infusions and to learn the defining characteristics of protists. We will also identify and characterize the protists in our transect. We then did serial dilutions to plate the bacteria and characterize the bacterial diversity. | ||
'''Materials and Methods | '''Materials and Methods | ||
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Procedure one involved making a wet mount from the Hay Infusion to observe with the microscope at 4x and 10x. Focus on an organism, describe it, and record its size. Use the Dichotomous Key to identify organisms. | Procedure one involved making a wet mount from the Hay Infusion to observe with the microscope at 4x and 10x. Focus on an organism, describe it, and record its size. Use the Dichotomous Key to identify organisms. | ||
Procedure two involved prepare for next week’s microbiology lab by preparing agar plates. | Procedure two involved prepare for next week’s microbiology lab by preparing agar plates. We obtained four nutrient agar and four nutrient agar plus tetracycline plates. We performed serial dilutions for the bacteria to grow on each plate. We added bacteria to each plate and let the plates sit at room temperature for a week. | ||
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Pictures of Protists: | Pictures of Protists: | ||
[[Image:bio210 drawings of protists.jpg]] | [[Image:bio210 drawings of protists.jpg]] | ||
'''Conclusion''' | |||
Revision as of 21:07, 4 February 2016
January 22, 2016 Exercise 2: Identifying Algae and Protists
Purpose The purpose of this lab is to use a Dichotomous Key to identify protists from our Hay Infusions and to learn the defining characteristics of protists. We will also identify and characterize the protists in our transect. We then did serial dilutions to plate the bacteria and characterize the bacterial diversity.
Materials and Methods Procedure one involved making a wet mount from the Hay Infusion to observe with the microscope at 4x and 10x. Focus on an organism, describe it, and record its size. Use the Dichotomous Key to identify organisms. Procedure two involved prepare for next week’s microbiology lab by preparing agar plates. We obtained four nutrient agar and four nutrient agar plus tetracycline plates. We performed serial dilutions for the bacteria to grow on each plate. We added bacteria to each plate and let the plates sit at room temperature for a week.
Data and Observations
Our Hay Infusion was filled halfway with brown dirty water. There was some dead plant life sitting at the bottom on top of sand or dirt that had collected at the bottom of the jar. All of the plant life looked destroyed. There was a sort of film over the surface of the water. We took samples from the top, middle and bottom of the Hay Infusion and viewed the samples under a microscope.
We used the Dichotomous Key to determine what protists were living in our Hay Infusion.
Description of cell from bottom of hay infusion: -Cell is colorless -Cell is stagnant -Cell is not spherical -Shape of cell remains constant -Seems as if cell has very thick cell walls -Parts of cell would range from 2-30 ocular spaces at 100x -This protist sample is a type of algae known as Pandorina.
Description of cell from middle layer of hay infusion: -4 ocular spaces (at 10x objective) -small body -oval shaped -fast swimmer -Colpidium
Description of cell from top layer of Hay Infusion: -random, quick movements, -cell compresses as it is moving -10 ocular spaces -Stentor
Another cell from top layer of Hay Infusion: -5 ocular spaces -colonies -Gloeocapsa
Another cell from top layer of Hay Infusion: -150 ocular spaces -resembles a large blob -Hydrodictyon
Conclusion
M.P.
January 15, 2016 Exercise 1: Examining Biological Life at AU
Purpose The purpose of this week's lab experiment was to examine and compare different members of the Volvocine Line and to examine the biodiversity on the campus of American University. At the end of the lab, we set up a Hay Infusion with abiotic and biotic compontnets found in our transect.
Materials and Methods Procedure one involved examining members of the Volvocine Line: Chlamydomonas, Gonium, and Volvox. We recorded the colony size, number of cells, specialization of these cells, and whether the cell was isogamous or oogamous under the microscope. Procedure two involved going to our transect and recording everything we observed. We also took samples of our transect and brought the samples back to the laboratory. Procedure three involved making a Hay Infusion. To do this, we placed 10-12 grams of our sample into a jar with 500 mLs of deerpark water, added 0.1 gram of dried milk, and mixed the jar for 10 seconds.
Data and Observations Transect Four included many different biotic and abiotic factors. The abiotic factors included rocks, water, soil and benches. The biotic factors included trees, bushes, flowers, fishes, and many organisms. This transect was interesting to survey and collect from. There is so much plant life and animal life, although we were only able to view the organisms under the microscope. A majority of the plant life was wilted and brown due to the cold weather.
Conclusion Transect four had many abiotic and biotic components to it. These abiotic and biotic components will help us examine the biodiversity at AU. Further experiments will show the different types of organisms that live in transect four at American University.
M.P.