User:Maiko Furubayashi: Difference between revisions

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*2005-2009 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (BS in Engineering)
*2005-2009 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (BS in Engineering)
*2009-2014 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (PhD in Engineering) Advisor: [http://chem.tf.chiba-u.jp/~umeno/ Daisuke Umeno]
*2009-2014 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (PhD in Engineering) Advisor: [http://chem.tf.chiba-u.jp/~umeno/ Daisuke Umeno]
**Doctoral Thesis: [http://mitizane.ll.chiba-u.jp/meta-bin/mt-pdetail.cgi?flm=0005189438801678&smode=1&cd=00117676&edm=0&tlang=0 Diversification and Specialization of Non-natural Carotenoid Biosynthetic Pathways by Directed Evolution / 進化分子工学による非天然カロテノイド生合成経路の多様化と特異化]
**Doctoral Thesis: [http://mitizane.ll.chiba-u.jp/meta-bin/mt-pdetail.cgi?flm=0005189438801678&smode=1&cd=00117676&edm=0&tlang=0 Diversification and Specialization of Non-natural Carotenoid Biosynthetic Pathways by Directed Evolution]
*2011-2014 Research Fellow (DC1) of the Japan Society for the Promotion of Science
*2011-2014 Research Fellow (DC1) of the Japan Society for the Promotion of Science
*2014- Postdoctoral Associate at Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology
*2014- Postdoctoral Associate at Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology
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==Presentations==
==Presentations==
===Oral===
===Oral===
#Furubayashi M, '''A highly specific synthetic metabolic pathway assembled from promiscuous enzymes'''. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 ([http://sb6.biobricks.org/speaker/maiko-furubayashi/ Invited: Lightning Talks Track 1])
#Furubayashi M, '''A highly specific synthetic metabolic pathway assembled from promiscuous enzymes'''. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 ([http://sb6.biobricks.org/speaker/maiko-furubayashi/ Lightning Talks Track 1])


===Poster===
===Poster===
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*[http://www16.plala.or.jp/x-tomo/ OSX Freewares]
*[http://www16.plala.or.jp/x-tomo/ OSX Freewares]
*DNA配列編集等
*DNA配列編集等
**'''[http://www.geneious.com/ Geneious]'''
**[http://www.geneious.com/ Geneious]
**[http://ja.invitrogen.com/site/jp/ja/home/Products-and-Services/Applications/Cloning/vector-nti-software/vector-nti-express-software.html Vector NTI Express] '''for mac!'''
**[http://ja.invitrogen.com/site/jp/ja/home/Products-and-Services/Applications/Cloning/vector-nti-software/vector-nti-express-software.html Vector NTI Express]
**[http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE: A plasmid Editor]
**[http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE: A plasmid Editor]
**[http://www.mekentosj.com/ mekentosj] : EnzymeX, 4Peaks
**[http://www.mekentosj.com/ mekentosj] : EnzymeX, 4Peaks
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*[http://lipidbank.jp/ Lipidbank]
*[http://lipidbank.jp/ Lipidbank]
*[http://cgsc.biology.yale.edu/index.php CGSC, The Coli Genetic Stock Center] genotype はここ
*[http://cgsc.biology.yale.edu/index.php CGSC, The Coli Genetic Stock Center] genotype はここ
===たんぱく===
*蛋白質二次構造予測
**[http://bioinf.cs.ucl.ac.uk/psipred/ PSIPRED]
*膜貫通領域予測
**[http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]
**[http://bp.nuap.nagoya-u.ac.jp/sosui/ SOSUI]
*αhelix helical wheel diagram
**[http://inn-temp.weizmann.ac.il/cgi-bin/emboss/pepwheel pepwheel]


===Promoter===
===Promoter===
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**[http://www.ncbi.nlm.nih.gov/pubmed/20843779 Davis JH 2010 NAR]
**[http://www.ncbi.nlm.nih.gov/pubmed/20843779 Davis JH 2010 NAR]
**[http://biofab.synberc.org/data BIOFAB]
**[http://biofab.synberc.org/data BIOFAB]
===RBS===
===RBS===
*[https://salis.psu.edu/software/ RBS calculator v1.1 from Salis Lab]
*[https://salis.psu.edu/software/ RBS calculator v1.1 from Salis Lab]
===レアコドン===
===レアコドン===
*[http://nihserver.mbi.ucla.edu/RACC/ Rare Codon Calculator]
*[http://nihserver.mbi.ucla.edu/RACC/ Rare Codon Calculator]
===たんぱく===
*蛋白質二次構造予測
**[http://bioinf.cs.ucl.ac.uk/psipred/ PSIPRED]
*膜貫通領域予測
**[http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]
**[http://bp.nuap.nagoya-u.ac.jp/sosui/ SOSUI]
*αhelix helical wheel diagram
**[http://inn-temp.weizmann.ac.il/cgi-bin/emboss/pepwheel pepwheel]
*GFP
**[https://greenfluorescentblog.wordpress.com/2012/08/01/folding-and-maturation-or-how-to-evolve-your-own-gfp-part-1/ Folding] and [https://greenfluorescentblog.wordpress.com/2012/10/17/folding-and-maturation-or-how-to-evolve-your-own-gfp-part-2/ maturation], or how to evolve your own GFP | greenfluorescentblog


===イースト===
===イースト===
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| width="34%" | <!-- ###################################################### 右 ###################################################### -->
| width="34%" | <!-- ###################################################### 右 ###################################################### -->
===おてがるクローニング===
===おてがるクローニング===
#[http://nebiocalculator.neb.com/#!/ NEBioCalculator]
#[http://j5.jbei.org/index.php/Main_Page j5]
#[http://j5.jbei.org/index.php/Main_Page j5]
#[http://www.nature.com/nbt/journal/v30/n5/full/nbt.2183.html RecET cloning: Fu 2009 Nat Biotechnol]
#Type IIS
#[http://www.nature.com/nmeth/journal/v4/n3/abs/nmeth1010.html sequence and ligation independent cloning (SLIC) using T4 DNApol: Li MZ 2007 Nat Meth]
##[http://www.sciencedirect.com/science/article/pii/S0168165608020804 FASTR (LguI) : Kotera I 2008 J Biotechnol] / [http://res.tagen.tohoku.ac.jp/FBC/FBCmember/FBC_NewsLetterNo30.pdf 日本語解説記事(生命化学研究レター)]←よりスモールスケール
#*[http://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/lic/ EMBLによるプロトコル]
#*[http://bitesizebio.com/articles/sequence-and-ligation-independent-cloning/ BitesizeBioの記事(口コミあり)]
#ligation independent cloning by PCR
##[http://www.biotechniques.com/BiotechniquesJournal/2010/June/Overlap-extension-PCR-cloning-a-simple-and-reliable-way-to-create-recombinant-plasmids/biotechniques-280116.html Overlap extension PCR cloning: Bryksin AV 2010 BioTechniques]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0006441 Circular Polymerase Extension Cloning: Quan 2009 PLoS ONE]
#digest and ligate in one pot using Type IIS restriction enzyme
##[http://www.sciencedirect.com/science/article/pii/S0168165608020804 FASTR (LguI) : Kotera I 2008 J Biotechnol]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0003647 Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0003647 Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0005553 Golden Gate Shuffling: Engler C 2009 PLoS ONE]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0005553 Golden Gate Shuffling: Engler C 2009 PLoS ONE]
#Gibson Assembly
#SLIC / Ligation Independent Cloning
#*[http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html Gibson DG 2009 Nat Meth]
##[http://www.nature.com/nmeth/journal/v4/n3/abs/nmeth1010.html Li 2007 Nat Method] / [http://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/lic/ EMBL] / [http://bitesizebio.com/articles/sequence-and-ligation-independent-cloning/ BitesizeBio] (T4 DNA pol)
##[http://aem.asm.org/content/early/2012/05/13/AEM.00844-12 One step SLIC]
##[http://www.biotechniques.com/BiotechniquesJournal/2007/September/In-Fusion-assembly-seamless-engineering-of-multidomain-fusion-proteins-modular-vectors-and-mutations/biotechniques-43102.html In-Fusion] (poxvirus DNA pol)
#Gibson
#*[http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html Gibson 2009 Nat Method]
#*[https://www.addgene.org/plasmid-protocols/gibson-assembly/ Addgene] / [https://www.neb.com/products/e2611-gibson-assembly-master-mix NEB] / [[Gibson Assembly|OWW]] / [https://www.idtdna.com/pages/docs/default-source/user-guides-and-protocols/gibson-assembly.pdf?sfvrsn=16 IDT]
#PCRベース
##[http://www.sciencedirect.com/science/article/pii/B9780123851208000176 MEGAWHOP library cloning 2011] [http://link.springer.com/protocol/10.1385%2F1-59259-395-X%3A23 2003] [http://www.biotechniques.com/multimedia/archive/00010/02335st03_10094a.pdf 2002]
##[http://www.biotechniques.com/BiotechniquesJournal/2010/June/Overlap-extension-PCR-cloning-a-simple-and-reliable-way-to-create-recombinant-plasmids/biotechniques-280116.html Overlap extension PCR]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0006441 Circular Polymerase Extension Cloning (CPEC)]
#リコンビニアリング
##yeast: [http://www.sciencedirect.com/science/article/pii/S0147619X09000675 Shanks RMQ 2009 Plasmid]
##[http://www.nature.com/nbt/journal/v30/n5/full/nbt.2183.html RecET cloning: Fu 2009 Nat Biotechnol]
##lambda RED on [http://www.biomedcentral.com/1471-2180/10/209 RK2]


===実験tips===
===実験tips===
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*[http://micro.fhw.oka-pu.ac.jp/microbiology/microbiology-index.html 微生物の世界 (Web講義室) - 岡山県立大学山本研究室]
*[http://micro.fhw.oka-pu.ac.jp/microbiology/microbiology-index.html 微生物の世界 (Web講義室) - 岡山県立大学山本研究室]
*[http://www.kenkyuu.net/biotechforum.html Bio Technical フォーラム - 研究留学ネット]
*[http://www.kenkyuu.net/biotechforum.html Bio Technical フォーラム - 研究留学ネット]
*[http://www.gelifesciences.co.jp/newsletter/biodirect_mail/technical_tips/ GEヘルスケア タンパク質実験tips]
**[http://www.gelifesciences.co.jp/newsletter/biodirect_mail/cell_story/?utm_source=rcmd 細胞夜話]
**[http://www.gelifesciences.co.jp/technologies/protein_preparation/index.html タンパク質のサンプル調製・前処理方法のコツ]
*[http://www.beckmancoulter.co.jp/product/product01/clipping_data/index.html ベックマン博士の一口メモ /ベックマン・コールター]
*[http://www.labome.com/method/Detergents-Triton-X-100-Tween-20-and-More.html Detergents: Triton X-100, Tween 20 and more]
===HPLC/GC===
===HPLC/GC===
*[http://www.gls.co.jp/technique/technique_data/usage_of_hplc/index.html HPLCの上手な使い方 | ジーエルサイエンス]
*[http://www.gls.co.jp/technique/technique_data/usage_of_hplc/index.html HPLCの上手な使い方 | ジーエルサイエンス]
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*[http://www7b.biglobe.ne.jp/~satoki/ronbun/kyo.html これから論文を書く若者のためのお経集]
*[http://www7b.biglobe.ne.jp/~satoki/ronbun/kyo.html これから論文を書く若者のためのお経集]
===Scrivener===
===Scrivener===
*[http://gwenhernandez.com/2011/03/29/tech-tuesday-formatting-tips-for-scrivener-2-x/ Formatting tips for scrivener]
*[http://gwenhernandez.com/scrivener-corner/scrivener-tips/ Scrivener Tips]
*[http://gwenhernandez.com/scrivener-corner/scrivener-tips/ Scrivener Tips]
*Properties > Formatting > Schrivenings
*Properties > Formatting > Schrivenings
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===日本語===
===日本語===
*[http://verbhandbook.ninjal.ac.jp/ 基本動詞ハンドブック - 国立国語研究所]
*[http://verbhandbook.ninjal.ac.jp/ 基本動詞ハンドブック - 国立国語研究所]
*[http://onomatopelabo.jp/ オノマトペラボ] / [http://www.ninjal.ac.jp/pages/onomatopoeia/ 東北オノマトペ]
*[http://onomatopelabo.jp/ オノマトペラボ]
*[http://www.amazon.co.jp/%E8%87%AA%E5%AE%B6%E8%A3%BD-%E6%96%87%E7%AB%A0%E8%AA%AD%E6%9C%AC-%E6%96%B0%E6%BD%AE%E6%96%87%E5%BA%AB-%E4%BA%95%E4%B8%8A-%E3%81%B2%E3%81%95%E3%81%97/dp/4101168199/ 自家製 文章読本 - 井上ひさし]
*[http://www.amazon.co.jp/%E8%87%AA%E5%AE%B6%E8%A3%BD-%E6%96%87%E7%AB%A0%E8%AA%AD%E6%9C%AC-%E6%96%B0%E6%BD%AE%E6%96%87%E5%BA%AB-%E4%BA%95%E4%B8%8A-%E3%81%B2%E3%81%95%E3%81%97/dp/4101168199/ 自家製 文章読本 - 井上ひさし]
*[http://matome.naver.jp/odai/2138287088839183201 爆発音がした] [http://anond.hatelabo.jp/20080506041614 *]
*[http://matome.naver.jp/odai/2138287088839183201 爆発音がした] [http://anond.hatelabo.jp/20080506041614 *]
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*[https://en.wikisource.org/wiki/The_Elements_of_Style The Elements of Style - William Strunk, Jr.]
*[https://en.wikisource.org/wiki/The_Elements_of_Style The Elements of Style - William Strunk, Jr.]
*[https://owl.english.purdue.edu/owl/ Purdue Online Writing Lab (OWL)]
*[https://owl.english.purdue.edu/owl/ Purdue Online Writing Lab (OWL)]
*[http://www.hemingwayapp.com/ Hemingway App]
===日本語と英語===
===日本語と英語===
*[https://www.s.u-tokyo.ac.jp/ja/story/newsletter/english/ 科学英語を考える(トム・ガリー准教授) - 東京大学理学部ニュース]
*[https://www.s.u-tokyo.ac.jp/ja/story/newsletter/english/ 科学英語を考える(トム・ガリー准教授) - 東京大学理学部ニュース]
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*[http://www.amazon.co.jp/%E7%B6%9A%E3%83%BB%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3-%E3%83%9E%E3%83%BC%E3%82%AF/dp/4004301394/ 続・日本人の英語 - マーク・ピーターセン]
*[http://www.amazon.co.jp/%E7%B6%9A%E3%83%BB%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3-%E3%83%9E%E3%83%BC%E3%82%AF/dp/4004301394/ 続・日本人の英語 - マーク・ピーターセン]
*[http://www.amazon.co.jp/%E5%AE%9F%E8%B7%B5-%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%9E%E3%83%BC%E3%82%AF%E3%83%BB%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3/dp/4004314208/ 実践 日本人の英語 - マーク・ピーターセン]
*[http://www.amazon.co.jp/%E5%AE%9F%E8%B7%B5-%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%9E%E3%83%BC%E3%82%AF%E3%83%BB%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3/dp/4004314208/ 実践 日本人の英語 - マーク・ピーターセン]
===フェローシップ===
*[http://hodotermopsis.seesaa.net/category/1660702-1.html 生物学・科学に関する雑感。: 助成・フェローシップ制度]
*[http://www.math.nagoya-u.ac.jp/~hamanaka/money.html 助成金・奨学金・顕彰情報 (ベータ版)]
*[http://88clinic.jimdo.com/%E4%BA%A4%E9%80%9A%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9/%E3%83%99%E3%83%AB%E3%82%AE%E3%83%BC%E7%95%99%E5%AD%A6%E6%89%8B%E7%B6%9A%E3%81%8D-%E6%84%9F%E6%83%B3/%E6%B5%B7%E5%A4%96%E7%95%99%E5%AD%A6%E5%8A%A9%E6%88%90%E9%87%91%E6%83%85%E5%A0%B1/ 海外留学助成金情報]
*[http://www.virus.kyoto-u.ac.jp/Lab/SugitaFolder/ess2.html 海外留学とフェローシップ | 京大ウイルス研・杉田研究室:エッセイ2]
*[http://scienceportal.jst.go.jp/funding/application/index.html ファンド(研究助成) | SciencePortal]
*[https://center6.umin.ac.jp/cgi-open-bin/josei/select/index.cgi?serv=jlist&func=search&nendo=now&order=end_date 研究費補助機関データベース管理システム]
*[http://www.rockefeller.edu/sr-pd/index.php?page=PostdocPrograms The Rockefeller University » Sponsored Research and Program Development]
*[http://www.fic.nih.gov/FUNDING/NONNIH/Pages/postdoctoral.aspx Postdoctoral Non-NIH Funding Opportunities - Fogarty International Center @ NIH]
*[http://www.onsf.uconn.edu/find-scholarships/opportunities-for-non-us-citizens/ Scholarships & Fellowships Open to Non-US Citizen Candidates | Office of National Scholarships & Fellowships]
*[http://cfr.ucsd.edu/postdoc/ Postdoctoral Funding Opportunities - UCSD]
*[http://grigoriefflab.janelia.org/fellowships Postdoctoral Funding Opportunities The Grigorieff Lab]
*[http://international.oregonstate.edu/sites/default/files/atosu/Selected_FundingSourcesOpentoNon-US-Citizens.pdf Selected Funding Opportunities Open to Non-US Citizens]


|}
|}
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*[http://www.sbj.or.jp/sbj/sbj_yomoyama.html 生物工学基礎講座-バイオよもやま話- | 公益社団法人 日本生物工学会]
*[http://www.sbj.or.jp/sbj/sbj_yomoyama.html 生物工学基礎講座-バイオよもやま話- | 公益社団法人 日本生物工学会]
*[http://www.brh.co.jp/seimeishi/ 季刊「生命誌」 | JT生命誌研究館]
*[http://www.brh.co.jp/seimeishi/ 季刊「生命誌」 | JT生命誌研究館]
*[https://nationalmaglab.org/education/magnet-academy Magnet Academy]
*[http://www.st.keio.ac.jp/learning/index.html 学問のすゝめ 理工学部 慶應義塾大学]
*[http://www.nature.com/collections/qghhqm Home : Statistics for Biologists]
*[http://waitbutwhy.com/ Wait But Why]
*[http://ww2.chemistry.gatech.edu/~lw26/structure/molecular_interactions/mol_int.html Molecular Interactions 分子間相互作用]
*[http://aasj.jp/watch.html 論文ウォッチ | AASJホームページ]
===教科書===
===教科書===
*[http://www.asmscience.org/content/journal/ecosalplus#backcontenthometab EcoSal Plus]
*[http://www.asmscience.org/content/journal/ecosalplus#backcontenthometab EcoSal Plus]
*[http://www.ncbi.nlm.nih.gov/books/NBK21766/ An Introduction to Genetic Analysis.] / [http://www.ncbi.nlm.nih.gov/books/NBK21942/ Conjugation]
*[http://www.ncbi.nlm.nih.gov/books/NBK21766/ An Introduction to Genetic Analysis.] / [http://www.ncbi.nlm.nih.gov/books/NBK21942/ Conjugation]
*[http://id.fnshr.info/2013/08/11/online-stat-books/ オンラインで無料で読める統計書22冊|Colorless Green Ideas]
*[http://id.fnshr.info/2013/08/11/online-stat-books/ オンラインで無料で読める統計書22冊|Colorless Green Ideas]
*[http://www.feynmanlectures.caltech.edu/ The Feynman Lectures on Physics]
*物理というか電磁気学
**[http://www.feynmanlectures.caltech.edu/ The Feynman Lectures on Physics]
**[http://www2.yamanashi-ken.ac.jp/~itoyo/lecture/electromagnetic/em-index.htm 電磁気学 | 伊藤 洋 山梨大学]
**[http://butsuri.fc2web.com/electro/electro.html 新イシカワ電磁気学]
**[http://homepage2.nifty.com/eman/electromag/contents.html EMANの電磁気学]
**[http://www.st.keio.ac.jp/learning/1312.html 電磁気学、ラーメンの湯気?]




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*[http://www7b.biglobe.ne.jp/~satoki/ronbun/ronbun.html 若手研究者のお経 -- これから論文を書く若者のために --]
*[http://www7b.biglobe.ne.jp/~satoki/ronbun/ronbun.html 若手研究者のお経 -- これから論文を書く若者のために --]
*[http://www2.phys.cs.is.nagoya-u.ac.jp/~tokita/tokiwiki/?%B8%A6%B5%E6%BC%BC%A4%CE%C1%AA%A4%D3%CA%FD 研究室の選び方 - Tokiwiki 2.0]
*[http://www2.phys.cs.is.nagoya-u.ac.jp/~tokita/tokiwiki/?%B8%A6%B5%E6%BC%BC%A4%CE%C1%AA%A4%D3%CA%FD 研究室の選び方 - Tokiwiki 2.0]
*[http://waitbutwhy.com/2015/05/elon-musk-the-worlds-raddest-man.html Elon Musk: The World's Raddest Man - Wait But Why]


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===休憩===
===休憩===
*[http://www.structuredprocrastination.com/index.php Structured Procrastination]
*[http://www.structuredprocrastination.com/index.php Structured Procrastination]
*[http://waitbutwhy.com/2013/10/why-procrastinators-procrastinate.html Why Procrastinators Procrastinate - Wait But Why]
*[https://www.philosophyguides.org/decoding/ Philosophy guides]
*[https://www.philosophyguides.org/decoding/ Philosophy guides]
*[http://www.gutenberg.org/ebooks/author/69 Sherlock Holmes] / [http://www.aozora.gr.jp/index_pages/person148.html 夏目漱石] / [http://www.aozora.gr.jp/index_pages/person42.html 寺田寅彦]
*[http://www.gutenberg.org/ebooks/author/69 Sherlock Holmes] / [http://www.aozora.gr.jp/index_pages/person148.html 夏目漱石] / [http://www.aozora.gr.jp/index_pages/person42.html 寺田寅彦]
*[http://honz.jp/list/mail/backnumber メールマガジン バックナンバー - HONZ]
*[http://www.realcoffee.jp/ リアルコーヒー]
*[http://www.realcoffee.jp/ リアルコーヒー]



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Maiko Furubayashi

  • 古林 真衣子
  • Postdoctoral Associate, Voigt Lab, MIT
  • maiko.furubayashi at gmail dot com



Publications

  1. Furubayashi M, Ikezumi M, Takaichi S, Maoka T, Hemmi H, Ogawa T, Saito K, Tobias AV, Umeno D. A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately-selective enzymes, Nature Commun., accepted
  2. Furubayashi M*, Li L*, Katabami A, Saito K, Umeno D. Directed evolution of squalene synthase for dehydrosqualene biosynthesis. FEBS Lett 588, 3375-3381 (2014)
  3. Furubayashi M, Ikezumi M, Kajiwara J, Iwasaki M, Fujii A, Li L, Saito K, Umeno D. A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption. PLoS ONE 9: e93317 (2014)
  4. Furubayashi M, Li L, Katabami A, Saito K, Umeno D, Construction of carotenoid biosynthetic pathways using squalene synthase. FEBS Lett 588, 436-442 (2014)
  5. Furubayashi M, Saito K, Umeno D, Evolutionary analysis of the functional plasticity of Staphylococcus aureus C30 carotenoid synthase, J Biosci Bioeng 117, (2014)
  6. Furubayashi M, Umeno D, Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids. Methods Mol Biol 892, 245-253 (2012) link
  7. Tashiro Y, Furubayashi M, Morijiri T, Suzuki K, Yasuno K, Matsuno S, Katabami A, Saito K, Umeno D, Escherichia coli robots that freeze, smell, swell, and time-keep. Synthetic Biology, IET., 1, 41-43 (2007)


Presentations

Oral

  1. Furubayashi M, A highly specific synthetic metabolic pathway assembled from promiscuous enzymes. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 (Lightning Talks Track 1)

Poster

  1. Furubayashi M, Li L. Takaichi S, Saito K and Umeno D, Evolutionary engineering of biosynthetic pathwaysfor non-natural C50 carotenoids. International Conference of Natural Products Biosynthesis (ICNPB), Awaji, Japan, June 17-22, 2012.
  2. Furubayashi M, Takaichi S, Misawa N, Umeno D, Specific non-natural C50 carotenoid pathways constructed by the combinatorial expression of laboratory-evolved enzymes. The 16th International Symposium on Carotenoids, Krakow, Poland, July 17 – 22, 2011.
  3. Furubayashi M, Ikezumi M, Saito K, Umeno D, Efficient and selective production of non-natural carotenoids by combinatorial expression of laboratory-evolved enzymes. Synthetic Biology 5.0 Conference (SB5.0), San Francisco, CA, June 2011
  4. Furubayashi M, Saito K, Umeno D, In-laboratory genetic drift of carotenoid synthase and its evolution of size specificity. The international chemical congress of Pacific basin societies (Pacifichem 2010), Honolulu, HI, December 2010
  5. Furubayashi M, Morijiri T, Karasawa T, Abe D, Ikezumi M, Fukushima S, Nakajima K, Yasuno K, Mihashi M, Kaneda T, Sugaya S, Katabami A, Matsuno S, Okuyama S, Umeno D, iGEM Chiba: Swimmy Bacteria, Aromatic E. coli, Balloon E. coli, and E. clock. iGEM Jamboree, Cambridge, MA, November 2006


Awards

  1. Research Fellow (DC1) of the Japan Society for the Promotion of Science, 2011-2014.
  2. Exemption from Repayment upon Graduation for Graduate School Students with Outstanding Result, JASSO Scholarship, 2009-2011.


研究業績(日本語)

論文・総説

  1. 古林真衣子, 梅野太輔, 人工代謝経路構築のための進化分子工学, 伏見譲(監修)進化分子工学〜高速分子進化によるタンパク質・核酸の開発〜, エヌ・ティー・エス (2013) link
  2. 梅野太輔, 冨永将大, 古林真衣子, 池紘平, 代謝経路と制御ネットワークの組織的な進化工学技術, 生物工学会誌 91, 333-336 (2013) TOC pdf
  3. 古林真衣子, 梅野太輔, 代謝ネットワーク建設のための進化分子工学―進化的デザインによる非天然代謝経路の創出―, 植田充美(監修)合成生物工学の隆起 ―有用物質の新たな生産法構築をめざして―, シーエムシー出版 (2012) link
  4. 古林真衣子, 田代洋平, 梅野太輔, 材料科学のための合成生物学, 未来材料, 10, 2-7 (2010) link
  5. 梅野太輔, 古林真衣子, 三沢典彦, 第3章・カロテノイドの生合成, 宮下和夫(監修)カロテノイドの科学と最新応用技術, シーエムシー出版, pp.27-37 (2009) link
  6. 梅野太輔, 田代洋平, 古林真衣子, 細胞のプログラム死と合成生物学. 極限環境微生物学会誌, 7, 31-35 (2008) link

特許

  1. 梅野太輔,古林真衣子,三沢典彦,高市真一「炭素数50のカロテノイドの製造方法」特願2011-130326 (2011/6/10)
  2. 梅野太輔,生悦住茉友,古林真衣子,方波見彰仁,斎藤恭一「テルペン合成酵素遺伝子のスクリーニング方法」特願 2009‐264294 (2009/11) / 特開2011-125334 (2011/6/30)

受賞

  1. ○古林真衣子,生悦住茉友,高市真一,斎藤恭一,梅野太輔「人工代謝経路によるC50-β-Caroteneの生合成」日本農芸化学会関東支部 2011年度大会,若手奨励賞(口頭発表・学生部門)
  2. iGEM Chiba,千葉大学 平成18年度課外活動等による学長表彰(コンペ部門)(2007)



べんりリンク

リンク集

ソフト

文献管理/SBM


データベース

Promoter

RBS

レアコドン

たんぱく

イースト

おてがるクローニング

  1. NEBioCalculator
  2. j5
  3. Type IIS
    1. FASTR (LguI) : Kotera I 2008 J Biotechnol / 日本語解説記事(生命化学研究レター)←よりスモールスケール
    2. Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE
    3. Golden Gate Shuffling: Engler C 2009 PLoS ONE
  4. SLIC / Ligation Independent Cloning
    1. Li 2007 Nat Method / EMBL / BitesizeBio (T4 DNA pol)
    2. One step SLIC
    3. In-Fusion (poxvirus DNA pol)
  5. Gibson
  6. PCRベース
    1. MEGAWHOP library cloning 2011 2003 2002
    2. Overlap extension PCR
    3. Circular Polymerase Extension Cloning (CPEC)
  7. リコンビニアリング
    1. yeast: Shanks RMQ 2009 Plasmid
    2. RecET cloning: Fu 2009 Nat Biotechnol
    3. lambda RED on RK2

実験tips

HPLC/GC

撮影

かきもの

スタイルガイド

論文の書き方

Scrivener

ワード

Markdown


日本語

英語

日本語と英語

フェローシップ

よみもの

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教科書


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休憩