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__NOTOC__
<html><p style="margin:0";><link rel="stylesheet" href="/index.php?title=User:Maiko_Furubayashi/style.css&action=raw&ctype=text/css" type="text/css" /></a></html>__NOTOC__
==Maiko Furubayashi==
*古林 真衣子
*Postdoctoral Associate, [http://web.mit.edu/voigtlab/ Voigt Lab, MIT]
*maiko.furubayashi at gmail dot com


==Maiko FURUBAYASHI / 古林真衣子==
*PhD student at [http://chem.tf.chiba-u.jp/~umeno/ Umeno lab], Chiba University, Japan
*Research Fellow (DC1) of the Japan Society for the Promotion of Science


===Contact===
*2005-2009 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (BS in Engineering)
*maiko.furubayashi *at* gmail.com
*2009-2014 Department of Applied Chemistry & Biotechnology, Chiba University, Japan (PhD in Engineering) Advisor: [http://chem.tf.chiba-u.jp/~umeno/ Daisuke Umeno]
*Dept. Applied Chemistry and Biotechnology, Chiba University
**Doctoral Thesis: [http://mitizane.ll.chiba-u.jp/meta-bin/mt-pdetail.cgi?flm=0005189438801678&smode=1&cd=00117676&edm=0&tlang=0 Diversification and Specialization of Non-natural Carotenoid Biosynthetic Pathways by Directed Evolution]
*1-33, Yayoi-cho, Inage, Chiba city, 263-8522, JAPAN
*2011-2014 Research Fellow (DC1) of the Japan Society for the Promotion of Science
*2014- Postdoctoral Associate at Synthetic Biology Center, Department of Biological Engineering, Massachusetts Institute of Technology


===Research Interests===
 
<!-- Feel free to add brief descriptions to your research interests as well -->
*[https://scholar.google.com/citations?user=JjNINBcAAAAJ&hl=en Google scholar citations] / [https://www.researchgate.net/profile/Maiko_Furubayashi ResearchGate] / [http://www.researcherid.com/rid/E-1457-2011 Researcher ID: E-1457-2011] / [http://orcid.org/0000-0001-5215-523X ORC ID: 0000-0001-5215-523X]
*Pathway Engineering / Synthetic Biology
*Isoprenoid pathway (Carotenoids/Steroids/Terpenoids)




==Publications==
==Publications==
#Furubayashi M, Saito K, Umeno D, '''Evolutionary analysis of the functional plasticity of ''Staphylococcus aureus'' C<sub>30</sub> carotenoid synthase''', ''in press''
#Furubayashi M, Ikezumi M, Takaichi S, Maoka T, Hemmi H, Ogawa T, Saito K, Tobias AV, Umeno D. '''A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately-selective enzymes''', ''Nature Commun.'', ''accepted''
#Furubayashi M, Umeno D, '''Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids'''. ''Methods Mol Biol'' '''892''' (2012) [http://www.springer.com/life+sciences/microbiology/book/978-1-61779-878-8 link]
#Furubayashi M*, Li L*, Katabami A, Saito K, Umeno D. '''Directed evolution of squalene synthase for dehydrosqualene biosynthesis'''. FEBS Lett 588, 3375-3381 (2014)
#Furubayashi M, Ikezumi M, Kajiwara J, Iwasaki M, Fujii A, Li L, Saito K, Umeno D. '''A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption.''' PLoS ONE 9: e93317 (2014)
#Furubayashi M, Li L, Katabami A, Saito K, Umeno D, '''Construction of carotenoid biosynthetic pathways using squalene synthase'''. FEBS Lett 588, 436-442 (2014)
#Furubayashi M, Saito K, Umeno D, '''Evolutionary analysis of the functional plasticity of Staphylococcus aureus C30 carotenoid synthase''', J Biosci Bioeng 117, (2014)
#Furubayashi M, Umeno D, '''Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids'''. ''Methods Mol Biol'' '''892''', 245-253 (2012) [http://www.springer.com/life+sciences/microbiology/book/978-1-61779-878-8 link]
#Tashiro Y, Furubayashi M, Morijiri T, Suzuki K, Yasuno K, Matsuno S, Katabami A, Saito K, Umeno D, '''Escherichia coli robots that freeze, smell, swell, and time-keep'''. ''Synthetic Biology, IET.'', '''1''', 41-43 (2007)
#Tashiro Y, Furubayashi M, Morijiri T, Suzuki K, Yasuno K, Matsuno S, Katabami A, Saito K, Umeno D, '''Escherichia coli robots that freeze, smell, swell, and time-keep'''. ''Synthetic Biology, IET.'', '''1''', 41-43 (2007)
#one paper about squalene/carotenoid pathway just about to submit :)
 
#one paper about pathway engineering under super long review process :(


==Presentations==
==Presentations==
===Oral===
===Oral===
#Furubayashi M, '''A highly specific synthetic metabolic pathway assembled from promiscuous enzymes'''. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 ([http://sb6.biobricks.org/speaker/maiko-furubayashi/ Invited: Lightning Talks Track 1])
#Furubayashi M, '''A highly specific synthetic metabolic pathway assembled from promiscuous enzymes'''. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 ([http://sb6.biobricks.org/speaker/maiko-furubayashi/ Lightning Talks Track 1])


===Poster===
===Poster===
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==日本語==
==研究業績（日本語）==
===論文・総説===
===論文・総説===
#古林真衣子, 梅野太輔, '''人工代謝経路構築のための進化分子工学''', 伏見譲（監修）進化分子工学〜高速分子進化によるタンパク質・核酸の開発〜, エヌ・ティー・エス (2013) [http://sv39.bestsystems.net/~dazjr000/item/detail/summary/bio/20131000_117.html link]
#古林真衣子, 梅野太輔, '''人工代謝経路構築のための進化分子工学''', 伏見譲（監修）進化分子工学〜高速分子進化によるタンパク質・核酸の開発〜, エヌ・ティー・エス (2013) [http://sv39.bestsystems.net/~dazjr000/item/detail/summary/bio/20131000_117.html link]
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#○古林真衣子，生悦住茉友，高市真一，斎藤恭一，梅野太輔「人工代謝経路によるC<sub>50</sub>-β-Caroteneの生合成」[http://jsbba.bt.a.u-tokyo.ac.jp/2011/11taikai-houkoku.html 日本農芸化学会関東支部 2011年度大会]，若手奨励賞（口頭発表・学生部門）
#○古林真衣子，生悦住茉友，高市真一，斎藤恭一，梅野太輔「人工代謝経路によるC<sub>50</sub>-β-Caroteneの生合成」[http://jsbba.bt.a.u-tokyo.ac.jp/2011/11taikai-houkoku.html 日本農芸化学会関東支部 2011年度大会]，若手奨励賞（口頭発表・学生部門）
#iGEM Chiba，千葉大学 平成１８年度課外活動等による学長表彰（コンペ部門）(2007)
#iGEM Chiba，千葉大学 平成１８年度課外活動等による学長表彰（コンペ部門）(2007)
==べんりリンク==
{| width="100%"
|- valign="top"
| width="33%" | <!-- ###################################################### 左 ###################################################### -->
===リンク集===
*[http://bip.weizmann.ac.il/toolbox/overview.html Weizmann Institute - BBCU - Toolbox]
*[http://mit.edu/voigtlab/webtools.html Voigt Lab | Web Tools]
*[http://www.jaffreylab.org/Pages/UsefulLinks.aspx Jaffrey Lab - Useful Links]
*[https://www.dna20.com/index.php?pageID=216 DNA2.0 bioinformatics toolbox]
*[http://www.genscript.com/tools.html Bioinformatics Tools - GenScript]
*[http://www.genomics.agilent.com/CollectionSubpage.aspx?PageType=Tool&SubPageType=ToolOverview&PageID=11 Agilent Tech Tools and Calculators]
===ソフト===
*[http://www16.plala.or.jp/x-tomo/ OSX Freewares]
*DNA配列編集等
**[http://www.geneious.com/ Geneious]
**[http://ja.invitrogen.com/site/jp/ja/home/Products-and-Services/Applications/Cloning/vector-nti-software/vector-nti-express-software.html Vector NTI Express]
**[http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE: A plasmid Editor]
**[http://www.mekentosj.com/ mekentosj] : EnzymeX, 4Peaks
**[https://www.dna20.com/genedesigner2/ Gene Designer from DNA2.0]
**[http://informagen.com/SA/ Sequence Analysis]
**[http://engels.genetics.wisc.edu/amplify/ Amplify]
*PDB viewer
**[http://www.pymol.org/ Pymol] "pymol 0.99"でぐぐると昔のfree verがみつかる
**[http://www.cgl.ucsf.edu/chimera/ UCSF chimera]
*グラフ描画
**[http://www.ochiailab.dnj.ynu.ac.jp/mjograph/indexj.html mjograph]
**[http://www.gnuplot.info/ gnuplot]
**[http://www.r-project.org/ R]
*http://mkweb.bcgsc.ca/
===文献管理/SBM===
*[http://www.mekentosj.com/ mekentosj] '''Papers'''
*[http://www.readcube.com/ readcube]
*[http://www.mendeley.com Mendeley]
*[http://www.citeulike.org/ CiteULike]
*[http://www.connotea.org/ Connotea]
*[http://www.zotero.org/ Zotero] (Firefox plugin)
| width="33%" | <!-- ###################################################### 中 ###################################################### -->
===データベース===
*[http://redpoll.pharmacy.ualberta.ca/CCDB/cgi-bin/STAT_NEW.cgi E. coli statistics] [http://ccdb.wishartlab.com/CCDB/cgi-bin/STAT_NEW.cgi *]
*[http://ecocyc.org/ ecocyc]
*[http://regulondb.ccg.unam.mx/ regulon DB]
*[http://www.genome.jp/kegg/ kegg]
*[http://www.kazusa.or.jp/codon/ codon usage database from kazusa]
*[http://lipidbank.jp/ Lipidbank]
*[http://cgsc.biology.yale.edu/index.php CGSC, The Coli Genetic Stock Center] genotype はここ
===Promoter===
*promoter prediction
**[http://www.fruitfly.org/seq_tools/promoter.html BDGP: Neural Network Promoter Prediction]
**[http://linux1.softberry.com/berry.phtml?topic=bprom&group=programs&subgroup=gfindb BPROM: prediction of bacterial promoters]
*constitutive promoters
**[http://partsregistry.org/wiki/index.php/Part:BBa_J23100 BBa_J23100-119]
**[http://www.ncbi.nlm.nih.gov/pubmed/20843779 Davis JH 2010 NAR]
**[http://biofab.synberc.org/data BIOFAB]
===RBS===
*[https://salis.psu.edu/software/ RBS calculator v1.1 from Salis Lab]
===レアコドン===
*[http://nihserver.mbi.ucla.edu/RACC/ Rare Codon Calculator]
===たんぱく===
*蛋白質二次構造予測
**[http://bioinf.cs.ucl.ac.uk/psipred/ PSIPRED]
*膜貫通領域予測
**[http://www.cbs.dtu.dk/services/TMHMM/ TMHMM]
**[http://bp.nuap.nagoya-u.ac.jp/sosui/ SOSUI]
*αhelix helical wheel diagram
**[http://inn-temp.weizmann.ac.il/cgi-bin/emboss/pepwheel pepwheel]
*GFP
**[https://greenfluorescentblog.wordpress.com/2012/08/01/folding-and-maturation-or-how-to-evolve-your-own-gfp-part-1/ Folding] and [https://greenfluorescentblog.wordpress.com/2012/10/17/folding-and-maturation-or-how-to-evolve-your-own-gfp-part-2/ maturation], or how to evolve your own GFP | greenfluorescentblog
===イースト===
*[http://wiki.yeastgenome.org/index.php/Commonly_used_auxotrophic_markers Commonly used auxotrophic markers - SGD Wiki]
| width="34%" | <!-- ###################################################### 右 ###################################################### -->
===おてがるクローニング===
#[http://nebiocalculator.neb.com/#!/ NEBioCalculator]
#[http://j5.jbei.org/index.php/Main_Page j5]
#Type IIS
##[http://www.sciencedirect.com/science/article/pii/S0168165608020804 FASTR (LguI) : Kotera I 2008 J Biotechnol] / [http://res.tagen.tohoku.ac.jp/FBC/FBCmember/FBC_NewsLetterNo30.pdf 日本語解説記事（生命化学研究レター）]←よりスモールスケール
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0003647 Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0005553 Golden Gate Shuffling: Engler C 2009 PLoS ONE]
#SLIC / Ligation Independent Cloning
##[http://www.nature.com/nmeth/journal/v4/n3/abs/nmeth1010.html Li 2007 Nat Method] / [http://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/lic/ EMBL] / [http://bitesizebio.com/articles/sequence-and-ligation-independent-cloning/ BitesizeBio] (T4 DNA pol)
##[http://aem.asm.org/content/early/2012/05/13/AEM.00844-12 One step SLIC]
##[http://www.biotechniques.com/BiotechniquesJournal/2007/September/In-Fusion-assembly-seamless-engineering-of-multidomain-fusion-proteins-modular-vectors-and-mutations/biotechniques-43102.html In-Fusion] (poxvirus DNA pol)
#Gibson
#*[http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html Gibson 2009 Nat Method]
#*[https://www.addgene.org/plasmid-protocols/gibson-assembly/ Addgene] / [https://www.neb.com/products/e2611-gibson-assembly-master-mix NEB] / [[Gibson Assembly|OWW]] / [https://www.idtdna.com/pages/docs/default-source/user-guides-and-protocols/gibson-assembly.pdf?sfvrsn=16 IDT]
#PCRベース
##[http://www.sciencedirect.com/science/article/pii/B9780123851208000176 MEGAWHOP library cloning 2011] [http://link.springer.com/protocol/10.1385%2F1-59259-395-X%3A23 2003] [http://www.biotechniques.com/multimedia/archive/00010/02335st03_10094a.pdf 2002]
##[http://www.biotechniques.com/BiotechniquesJournal/2010/June/Overlap-extension-PCR-cloning-a-simple-and-reliable-way-to-create-recombinant-plasmids/biotechniques-280116.html Overlap extension PCR]
##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0006441 Circular Polymerase Extension Cloning (CPEC)]
#リコンビニアリング
##yeast: [http://www.sciencedirect.com/science/article/pii/S0147619X09000675 Shanks RMQ 2009 Plasmid]
##[http://www.nature.com/nbt/journal/v30/n5/full/nbt.2183.html RecET cloning: Fu 2009 Nat Biotechnol]
##lambda RED on [http://www.biomedcentral.com/1471-2180/10/209 RK2]
===実験tips===
*[http://bitesizebio.com/ Bitesize bio]
*[http://www.neb.com/nebecomm/tech_reference/default.asp NEB technical reference]
*[http://cse.fra.affrc.go.jp/ksaitoh/techtips.html K.saito氏のちょいテク]：[http://cse.fra.affrc.go.jp/ksaitoh/economy3100.html ABI3100けちけちtips]
*[http://catalog.takara-bio.co.jp/techinfo/techinfo.asp?mode=1 TAKARAバイオ テクニカルサポート]
*[http://staff.aist.go.jp/s-hanai/link1.html#03 プライマーデザインのコツ - 花井＠産総研]
*[http://micro.fhw.oka-pu.ac.jp/microbiology/microbiology-index.html 微生物の世界 (Web講義室) - 岡山県立大学山本研究室]
*[http://www.kenkyuu.net/biotechforum.html Bio Technical フォーラム - 研究留学ネット]
*[http://www.gelifesciences.co.jp/newsletter/biodirect_mail/technical_tips/ GEヘルスケア タンパク質実験tips]
**[http://www.gelifesciences.co.jp/newsletter/biodirect_mail/cell_story/?utm_source=rcmd 細胞夜話]
**[http://www.gelifesciences.co.jp/technologies/protein_preparation/index.html タンパク質のサンプル調製・前処理方法のコツ]
*[http://www.beckmancoulter.co.jp/product/product01/clipping_data/index.html ベックマン博士の一口メモ /ベックマン・コールター]
*[http://www.labome.com/method/Detergents-Triton-X-100-Tween-20-and-More.html Detergents: Triton X-100, Tween 20 and more]
===HPLC/GC===
*[http://www.gls.co.jp/technique/technique_data/usage_of_hplc/index.html HPLCの上手な使い方　|　ジーエルサイエンス]
*[http://www.shodex.com/japanese/dso.html Ｓｈｉｎｏちゃんの液クロＳＯＳ]
*[http://www.an.shimadzu.co.jp/hplc/support/faq/faq.htm 島津 高速液体クロマトグラフィー分析(液クロ)の留意点，入門 : 株式会社島津製作所]
*[http://www.gls.co.jp/technique/technique_data/basics_of_gc/index.html ガスクロマトグラフィーの基礎　|　ジーエルサイエンス]
===撮影===
*[http://shuffle.genkosha.com/technique/lighting/ 玉ちゃんのライティング話]
*[http://aska-sg.net/butsuphoto01/002-20111221.html ライトボックス/ディフューザーの自作]
*[http://www.sensor.co.jp/gazo/ 画像処理の基本 - キーエンス sensor.co.jp]
|}
==かきもの==
{| width="100%"
|- valign="top"
| width="50%" | <!-- ###################################################### 執筆左 ###################################################### -->
===スタイルガイド===
*[http://www.ncbi.nlm.nih.gov/books/NBK988/ The NCBI Style Guide]
**[http://www.ncbi.nlm.nih.gov/books/NBK995/ Chapter 5 Style Points and Conventions] - AA/nt 表記，イタリック，カンマ，数字など
*genotype
**[http://jb.asm.org/site/misc/journal-ita_nom.xhtml INSTRUCTIONS TO AUTHORS - Journal of Bacteriology]
**[http://www.yeastgenome.org/sgdpub/Saccharomyces_cerevisiae.pdf Yeast (pdf)]
**[http://ecoliwiki.net/colipedia/index.php/Help:Genetic_nomenclature Genetic nomenclature - E.coli wiki]
===論文・文章の書き方===
*[http://www.igs.umaryland.edu/labs/hotopp/about/writing-2/writing-checklist/ Writing | Dr. Julie Dunning Hotopp]
*[http://www.biomedcentral.com/authors/authoracademy BioMed Central author academy]
*[http://www.americanscientist.org/issues/pub/the-science-of-scientific-writing The Science of Scientific Writing - American Scientist]
*[http://owll.massey.ac.nz/main/academic-writing.php Academic Writing - Massay University]
*[http://www.amazon.co.jp/%E7%90%86%E7%A7%91%E7%B3%BB%E3%81%AE%E4%BD%9C%E6%96%87%E6%8A%80%E8%A1%93-%E4%B8%AD%E5%85%AC%E6%96%B0%E6%9B%B8-624-%E6%9C%A8%E4%B8%8B-%E6%98%AF%E9%9B%84/dp/4121006240/ 理科系の作文技術 - 木下 是雄]
*[http://www7b.biglobe.ne.jp/~satoki/ronbun/kyo.html これから論文を書く若者のためのお経集]
===Scrivener===
*[http://gwenhernandez.com/2011/03/29/tech-tuesday-formatting-tips-for-scrivener-2-x/ Formatting tips for scrivener]
*[http://gwenhernandez.com/scrivener-corner/scrivener-tips/ Scrivener Tips]
*Properties > Formatting > Schrivenings
===ワード===
*[https://support.office.com/ja-jp/article/Word-%E3%81%AE%E3%82%B7%E3%83%A7%E3%83%BC%E3%83%88%E3%82%AB%E3%83%83%E3%83%88-%E3%82%AD%E3%83%BC-c0ca851f-3d58-4ce0-9867-799df73666a7?ui=ja-JP&rs=ja-JP&ad=JP ショートカット for mac]
*[https://support.office.com/ja-jp/article/%E6%AD%A3%E8%A6%8F%E8%A1%A8%E7%8F%BE%E3%82%92%E4%BD%BF%E7%94%A8%E3%81%97%E3%81%A6%E6%96%87%E5%AD%97%E5%88%97%E3%82%92%E6%A4%9C%E7%B4%A2%E3%81%8A%E3%82%88%E3%81%B3%E7%BD%AE%E6%8F%9B%E3%81%99%E3%82%8B-%E9%AB%98%E5%BA%A6-eeaa03b0-e9f3-4921-b1e8-85b0ad1c427f?CorrelationId=bb487913-6eb2-4853-b924-56c2da8952c8&ui=ja-JP&rs=ja-JP&ad=JP#__toc286834988 正規表現・ワイルドカード]
*フィールドの削除：command + shift + F9
===マークアップ／プログラミング===
*Markdown
**[http://daringfireball.net/projects/markdown/ By JOHN GRUBER]
**[https://github.com/adam-p/markdown-here/wiki/Markdown-Cheatsheet Markdown Cheatsheet] / [https://github.com/adam-p/markdown-here/wiki/Markdown-Here-Cheatsheet Markdown Here Cheetsheet]
**[http://jekyllrb.com/ Jekyll]
*[http://ogp.me/ The Open Graph protocol]
*[http://matplotlib.org/resources/index.html External Resources — Matplotlib 1.5.1 documentation]
*[http://brackets.io/ Brackets]
*[http://ignorethecode.net/blog/2010/04/20/footnotes/ ignore the code: Footnotes] / [http://www.leancrew.com/all-this/2010/05/popup-footnotes/ Popup footnotes - All this]
*[https://github.com/chjj/marked Markdown.js]
===ず===
*[http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1003833 PLOS Computational Biology: Ten Simple Rules for Better Figures]
*[http://www.kennethmoreland.com/color-advice/ Color Map Advice for Scientific Visualization - Kenneth Moreland]
| width="50%" | <!-- ###################################################### 執筆右 ###################################################### -->
===日本語===
*[http://verbhandbook.ninjal.ac.jp/ 基本動詞ハンドブック - 国立国語研究所]
*[http://onomatopelabo.jp/ オノマトペラボ]
*[http://www.amazon.co.jp/%E8%87%AA%E5%AE%B6%E8%A3%BD-%E6%96%87%E7%AB%A0%E8%AA%AD%E6%9C%AC-%E6%96%B0%E6%BD%AE%E6%96%87%E5%BA%AB-%E4%BA%95%E4%B8%8A-%E3%81%B2%E3%81%95%E3%81%97/dp/4101168199/ 自家製 文章読本 - 井上ひさし]
*[http://matome.naver.jp/odai/2138287088839183201 爆発音がした] [http://anond.hatelabo.jp/20080506041614 *]
*[http://logoon.org/ 文体診断ロゴーン] / [http://www.ennach.sakura.ne.jp/ 小説形態素解析CGI（β）]
*[http://jibun.atmarkit.co.jp/lskill01/index/index_kokugo.html ITエンジニアにも必要な国語力 インデックス － ＠IT自分戦略研究所]
*[http://dl.ndl.go.jp/ 国立国会図書館デジタルコレクション] / [http://kindai.ndl.go.jp/ 近代デジタルライブラリー]
*[http://pj.ninjal.ac.jp/gairaigo/ 「外来語」言い換え提案 | 国立国語研究所]
===英語===
*[https://en.wikisource.org/wiki/The_Elements_of_Style The Elements of Style - William Strunk, Jr.]
*[https://owl.english.purdue.edu/owl/ Purdue Online Writing Lab (OWL)]
*[http://www.hemingwayapp.com/ Hemingway App]
*[http://www.quickanddirtytips.com/grammar-girl Grammar Girl - Quick and Dirty Tips]
*[http://writeordie.com/ Write or Die]
===日本語と英語===
*[https://www.s.u-tokyo.ac.jp/ja/story/newsletter/english/ 科学英語を考える（トム・ガリー准教授） - 東京大学理学部ニュース]
**[https://www.s.u-tokyo.ac.jp/ja/story/newsletter/english/10.html 第10回 英語らしい英語を書くコツ]
*[http://www.amazon.co.jp/%E6%97%A5%E6%9C%AC%E8%AA%9E%E3%81%AE%E8%AB%96%E7%90%86-%E4%B8%AD%E5%85%AC%E6%96%87%E5%BA%AB-%E5%A4%96%E5%B1%B1-%E6%BB%8B%E6%AF%94%E5%8F%A4/dp/4122014697 日本語の論理 - 外山滋比古]
*[http://www.amazon.co.jp/%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%9E%E3%83%BC%E3%82%AF%E3%83%BB%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3/dp/4004300185/ 日本人の英語 - マーク・ピーターセン]
*[http://www.amazon.co.jp/%E7%B6%9A%E3%83%BB%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3-%E3%83%9E%E3%83%BC%E3%82%AF/dp/4004301394/ 続・日本人の英語 - マーク・ピーターセン]
*[http://www.amazon.co.jp/%E5%AE%9F%E8%B7%B5-%E6%97%A5%E6%9C%AC%E4%BA%BA%E3%81%AE%E8%8B%B1%E8%AA%9E-%E5%B2%A9%E6%B3%A2%E6%96%B0%E6%9B%B8-%E3%83%9E%E3%83%BC%E3%82%AF%E3%83%BB%E3%83%94%E3%83%BC%E3%82%BF%E3%83%BC%E3%82%BB%E3%83%B3/dp/4004314208/ 実践 日本人の英語 - マーク・ピーターセン]
===フェローシップ===
*[http://hodotermopsis.seesaa.net/category/1660702-1.html 生物学・科学に関する雑感。: 助成・フェローシップ制度]
*[http://www.math.nagoya-u.ac.jp/~hamanaka/money.html 助成金・奨学金・顕彰情報 (ベータ版)]
*[http://88clinic.jimdo.com/%E4%BA%A4%E9%80%9A%E3%82%A2%E3%82%AF%E3%82%BB%E3%82%B9/%E3%83%99%E3%83%AB%E3%82%AE%E3%83%BC%E7%95%99%E5%AD%A6%E6%89%8B%E7%B6%9A%E3%81%8D-%E6%84%9F%E6%83%B3/%E6%B5%B7%E5%A4%96%E7%95%99%E5%AD%A6%E5%8A%A9%E6%88%90%E9%87%91%E6%83%85%E5%A0%B1/ 海外留学助成金情報]
*[http://www.virus.kyoto-u.ac.jp/Lab/SugitaFolder/ess2.html 海外留学とフェローシップ | 京大ウイルス研・杉田研究室：エッセイ２]
*[http://scienceportal.jst.go.jp/funding/application/index.html ファンド(研究助成) | SciencePortal]
*[https://center6.umin.ac.jp/cgi-open-bin/josei/select/index.cgi?serv=jlist&func=search&nendo=now&order=end_date 研究費補助機関データベース管理システム]
*[http://www.rockefeller.edu/sr-pd/index.php?page=PostdocPrograms The Rockefeller University » Sponsored Research and Program Development]
*[http://www.fic.nih.gov/FUNDING/NONNIH/Pages/postdoctoral.aspx Postdoctoral Non-NIH Funding Opportunities - Fogarty International Center @ NIH]
*[http://www.onsf.uconn.edu/find-scholarships/opportunities-for-non-us-citizens/ Scholarships & Fellowships Open to Non-US Citizen Candidates | Office of National Scholarships & Fellowships]
*[http://cfr.ucsd.edu/postdoc/ Postdoctoral Funding Opportunities - UCSD]
*[http://grigoriefflab.janelia.org/fellowships Postdoctoral Funding Opportunities The Grigorieff Lab]
*[http://international.oregonstate.edu/sites/default/files/atosu/Selected_FundingSourcesOpentoNon-US-Citizens.pdf Selected Funding Opportunities Open to Non-US Citizens]
|}
==よみもの==
{| width="100%"
|- valign="top"
| width="33%" | <!-- ###################################################### よみもの左 ###################################################### -->
===すばらしい===
*[http://www.paulgraham.com/say.html What You Can't Say - Paul Graham]
*[http://waitbutwhy.com/2014/10/religion-for-the-nonreligious.html Religion for the Nonreligious - Wait But Why]
*[https://youtu.be/IlU-zDU6aQ0?list=PLudo29DC5MMzHS5e_NWQFN_-xJiUzssjR Marty Lobdell - Study Less Study Smart - YouTube]
*徒然草 [http://tsurezuregusa.com/ 吾妻利秋訳] / [http://tsurezuregusa.com/150dan/  第百五十段] / [http://tsurezuregusa.com/about/ このページについて]
*[https://vimeo.com/167796382 Bullshit featuring Harry Frankfurt]
*[http://saruwakakun.com/ サルワカくん] / [http://saruwakakun.com/design/photoshop Photoshop] / [http://saruwakakun.com/html-css/basic CSS]
===サイエンス===
*[http://schaechter.asmblog.org/ Small Things Considered]
*[http://bitesizebio.com/ Bitesize Bio]
*[http://dsc.discovery.com/technology/slideshow/synthetic-biologists-darwin.html How Would You Explain Synthetic Biology to Darwin?]
*[http://www.nite.go.jp/nbrc/cultures/others/nbrcnews/nbrcnews.html NBRCニュース]
*[http://www.sbj.or.jp/sbj/sbj_yomoyama.html 生物工学基礎講座－バイオよもやま話－ | 公益社団法人 日本生物工学会]
**[http://www.sbj.or.jp/sbj/sbj_yomoyama_2.html 続・生物工学基礎講座－バイオよもやま話－ | 公益社団法人 日本生物工学会]
*[http://www.brh.co.jp/seimeishi/ 季刊「生命誌」 | JT生命誌研究館]
*[https://nationalmaglab.org/education/magnet-academy Magnet Academy]
*[http://www.st.keio.ac.jp/learning/index.html 学問のすゝめ 理工学部 慶應義塾大学]
*[http://www.nature.com/collections/qghhqm Home : Statistics for Biologists]
*[http://waitbutwhy.com/ Wait But Why]
*[http://ww2.chemistry.gatech.edu/~lw26/structure/molecular_interactions/mol_int.html Molecular Interactions 分子間相互作用]
*[http://aasj.jp/watch.html 論文ウォッチ | AASJホームページ]
*[http://galileo.rice.edu/index.html The Galileo Project]
*[http://www.ub.edu/prometheus21/articulos/obsprometheus/BOULDING.pdf The Economics of the Coming Spaceship Earth]
=== Origin of stuff ===
*[http://bmcbiol.biomedcentral.com/articles/10.1186/s12915-014-0076-2 An inside-out origin for the eukaryotic cell | BMC Biology]
*[https://academic.oup.com/femsre/article/18/2-3/173/478683/On-the-origin-of-respiration-electron-transport On the origin of respiration: electron transport proteins from archaea to man]
*[http://www.its.caltech.edu/~bi1/Bi1__Micro-_to_Macro-Biology/Additional_Readings_files/Between%20a%20Rock%20and%20a%20Hard%20Place.pdf Between a Rock and a Hard Place / Günter Wächtershäuser]
*[https://en.wikipedia.org/wiki/Iron%E2%80%93sulfur_world_hypothesis Iron–sulfur world hypothesis - Wikipedia]
*[http://www.sciencedirect.com/science/article/pii/S0092867412014389 The Origin of Membrane Bioenergetics]
*[https://en.wikipedia.org/wiki/Lost_City_Hydrothermal_Field Lost City Hydrothermal Field - Wikipedia]
| width="34%" | <!-- ###################################################### よみもの中 ###################################################### -->
===ヒューマン===
*[http://www.aozora.gr.jp/cards/001234/files/46340_24939.html 愛知敬一 ファラデーの伝 電気学の泰斗]
*[http://www.brh.co.jp/s_library/j_site/scientistweb/ サイエンティストライブラリー：特別編 | JT生命誌研究館]
*[http://new2.immunoreg.jp/modules/pico_hint/ 慶應大学吉村研究室メール集]
*[http://www.bioreg.kyushu-u.ac.jp/saibou/message.html 九州大学 生体防御医学研究所 分子医科学分野 中山研究室]
**[http://www.bioreg.kyushu-u.ac.jp/saibou/qanda.html 幻の原稿編]
*[http://www.nig.ac.jp/section/interview.html インタビュー - 国立遺伝学研究所]
*[http://gakuiryugaku.net/newsletter JGSAU – 米国大学院学生会 | 現役留学生による直接支援 |  Newsletter]
*[http://www.sssj.org/jsssj/rensaikikaku/ResearchAbroad/ResearchAbroad_index.htm 海外研究体験記 - 表面化学]
*[http://www.jst.go.jp/kisoken/presto/nadeshiko/index.html なでしこ：女性研究者からのメッセージ]
*[http://www7b.biglobe.ne.jp/~satoki/ronbun/ronbun.html 若手研究者のお経 -- これから論文を書く若者のために --]
*[http://www2.phys.cs.is.nagoya-u.ac.jp/~tokita/tokiwiki/?%B8%A6%B5%E6%BC%BC%A4%CE%C1%AA%A4%D3%CA%FD 研究室の選び方 - Tokiwiki 2.0]
*[http://waitbutwhy.com/2015/05/elon-musk-the-worlds-raddest-man.html Elon Musk: The World's Raddest Man - Wait But Why]
*[http://www.webofstories.com/ Web of Stories]
*[http://shinbun.fan-miyagi.jp/article/article_20120119.php#container 伊達宗行さん（物理学者）に聞く：科学って、そもそも何だろう？｜宮城の新聞 ]
===教科書===
*[http://www.asmscience.org/content/journal/ecosalplus#backcontenthometab EcoSal Plus]
*[http://www.ncbi.nlm.nih.gov/books/NBK21766/ An Introduction to Genetic Analysis.] / [http://www.ncbi.nlm.nih.gov/books/NBK21942/ Conjugation]
*[http://id.fnshr.info/2013/08/11/online-stat-books/ オンラインで無料で読める統計書22冊｜Colorless Green Ideas]
*電磁気学
**[http://www.feynmanlectures.caltech.edu/ The Feynman Lectures on Physics]
**[http://www2.yamanashi-ken.ac.jp/~itoyo/lecture/electromagnetic/em-index.htm 電磁気学 | 伊藤 洋 山梨大学]
**[http://butsuri.fc2web.com/electro/electro.html 新イシカワ電磁気学]
**[http://homepage2.nifty.com/eman/electromag/contents.html ＥＭＡＮの電磁気学]
**[http://www.st.keio.ac.jp/learning/1312.html 電磁気学、ラーメンの湯気？]
*[https://www.rikanenpyo.jp/index.html 理科年表]
| width="33%" | <!-- ###################################################### よみもの右 ###################################################### -->
===ラボライフハック===
*[http://wws.weizmann.ac.il/mcb/UriAlon/materials-nurturing-scientists Materials for nurturing scientists | Uri Alon]
*[http://www.markowetzlab.org/skills/ Non-technical scientific skills no one bothered to teach you before]
*[https://www.hhmi.org/programs/resources-early-career-scientist-development Resources for the Development of Early-Career Scientists | HHMI.org]
*[http://www.phdcomics.com/comics/archive.php?comicid=1047 Average time spent composing one email]
*[http://www.nerdfitness.com/blog/2013/02/25/the-20-second-challenge/ The 20-second beast mode]
*[http://inoveryourhead.net/the-complete-guide-to-not-giving-a-fuck/ The complete guide to not giving a fuck]
*[http://www.raptitude.com/2014/07/not-giving-a-shit/ The elegant art of not giving a shit]
*[https://markmanson.net/books/subtle-art The Subtle Art of Not Giving a F*ck - Mark Manson]
*[http://www.nytimes.com/2015/10/26/your-money/learning-to-deal-with-the-impostor-syndrome.html?_r=0 Learning to Deal With] the [https://counseling.caltech.edu/general/InfoandResources/Impostor Impostor Syndrome]
===Procrastination===
*[http://www.structuredprocrastination.com/index.php Structured Procrastination]
*[http://waitbutwhy.com/2013/10/why-procrastinators-procrastinate.html Why Procrastinators Procrastinate - Wait But Why]
*[https://what-if.xkcd.com/ What if?] - [http://xkcd.com/ xkcd]
*[https://www.philosophyguides.org/decoding/ Philosophy guides]
*[http://www.gutenberg.org/ebooks/author/69 Sherlock Holmes] / [http://www.aozora.gr.jp/index_pages/person148.html 夏目漱石] / [http://www.aozora.gr.jp/index_pages/person42.html 寺田寅彦]
*[http://www.php.co.jp/shuzo/ 日めくり修造]
*[http://1000ya.isis.ne.jp/top/ 松岡正剛の千夜千冊]
*[http://honz.jp/list/mail/backnumber メールマガジン バックナンバー - HONZ]
*[http://www.realcoffee.jp/ リアルコーヒー]
*[http://topdocumentaryfilms.com/ Top Documentary Films - Watch Free Documentaries Online]
*[https://ja.wikipedia.org/wiki/%E4%B8%96%E7%95%8C%E6%9C%80%E5%8F%A4%E3%81%AE%E4%B8%80%E8%A6%A7 世界最古の一覧]
*[http://sebpearce.com/bullshit/  New-Age Bullshit Generator]
*[https://en.wikipedia.org/wiki/Timeline_of_human_evolution Timeline of human evolution]
*[https://en.wikipedia.org/wiki/Hypothetical_types_of_biochemistry Hypothetical types of biochemistry]
|}




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===links===
 
*[http://chem.tf.chiba-u.jp/~umeno/ Umeno lab, Chiba Univ (japanese)]
*[http://chem.tf.chiba-u.jp/igem Chiba iGEM]
*[http://chem.tf.chiba-u.jp/igem chiba igem website]
*[[User:Maiko_Furubayashi/style.css]]
===CSS===
*[[Maiko_Furubayashi/Chiba.css]]
*[[Maiko_Furubayashi/Chiba.css]]
*[[Maiko_Furubayashi/Chiba2.css]]
*[[Maiko_Furubayashi/Chiba2.css]]

Revision as of 11:18, 1 March 2017

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Maiko Furubayashi

  • 古林 真衣子
  • Postdoctoral Associate, Voigt Lab, MIT
  • maiko.furubayashi at gmail dot com




Publications

  1. Furubayashi M, Ikezumi M, Takaichi S, Maoka T, Hemmi H, Ogawa T, Saito K, Tobias AV, Umeno D. A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately-selective enzymes, Nature Commun., accepted
  2. Furubayashi M*, Li L*, Katabami A, Saito K, Umeno D. Directed evolution of squalene synthase for dehydrosqualene biosynthesis. FEBS Lett 588, 3375-3381 (2014)
  3. Furubayashi M, Ikezumi M, Kajiwara J, Iwasaki M, Fujii A, Li L, Saito K, Umeno D. A High-Throughput Colorimetric Screening Assay for Terpene Synthase Activity Based on Substrate Consumption. PLoS ONE 9: e93317 (2014)
  4. Furubayashi M, Li L, Katabami A, Saito K, Umeno D, Construction of carotenoid biosynthetic pathways using squalene synthase. FEBS Lett 588, 436-442 (2014)
  5. Furubayashi M, Saito K, Umeno D, Evolutionary analysis of the functional plasticity of Staphylococcus aureus C30 carotenoid synthase, J Biosci Bioeng 117, (2014)
  6. Furubayashi M, Umeno D, Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids. Methods Mol Biol 892, 245-253 (2012) link
  7. Tashiro Y, Furubayashi M, Morijiri T, Suzuki K, Yasuno K, Matsuno S, Katabami A, Saito K, Umeno D, Escherichia coli robots that freeze, smell, swell, and time-keep. Synthetic Biology, IET., 1, 41-43 (2007)


Presentations

Oral

  1. Furubayashi M, A highly specific synthetic metabolic pathway assembled from promiscuous enzymes. Synthetic Biology 6.0 Conference (SB6.0), Imperial Collage London, July 2013 (Lightning Talks Track 1)

Poster

  1. Furubayashi M, Li L. Takaichi S, Saito K and Umeno D, Evolutionary engineering of biosynthetic pathwaysfor non-natural C50 carotenoids. International Conference of Natural Products Biosynthesis (ICNPB), Awaji, Japan, June 17-22, 2012.
  2. Furubayashi M, Takaichi S, Misawa N, Umeno D, Specific non-natural C50 carotenoid pathways constructed by the combinatorial expression of laboratory-evolved enzymes. The 16th International Symposium on Carotenoids, Krakow, Poland, July 17 – 22, 2011.
  3. Furubayashi M, Ikezumi M, Saito K, Umeno D, Efficient and selective production of non-natural carotenoids by combinatorial expression of laboratory-evolved enzymes. Synthetic Biology 5.0 Conference (SB5.0), San Francisco, CA, June 2011
  4. Furubayashi M, Saito K, Umeno D, In-laboratory genetic drift of carotenoid synthase and its evolution of size specificity. The international chemical congress of Pacific basin societies (Pacifichem 2010), Honolulu, HI, December 2010
  5. Furubayashi M, Morijiri T, Karasawa T, Abe D, Ikezumi M, Fukushima S, Nakajima K, Yasuno K, Mihashi M, Kaneda T, Sugaya S, Katabami A, Matsuno S, Okuyama S, Umeno D, iGEM Chiba: Swimmy Bacteria, Aromatic E. coli, Balloon E. coli, and E. clock. iGEM Jamboree, Cambridge, MA, November 2006


Awards

  1. Research Fellow (DC1) of the Japan Society for the Promotion of Science, 2011-2014.
  2. Exemption from Repayment upon Graduation for Graduate School Students with Outstanding Result, JASSO Scholarship, 2009-2011.


研究業績（日本語）

論文・総説

  1. 古林真衣子, 梅野太輔, 人工代謝経路構築のための進化分子工学, 伏見譲（監修）進化分子工学〜高速分子進化によるタンパク質・核酸の開発〜, エヌ・ティー・エス (2013) link
  2. 梅野太輔, 冨永将大, 古林真衣子, 池紘平, 代謝経路と制御ネットワークの組織的な進化工学技術, 生物工学会誌 91, 333-336 (2013) TOC pdf
  3. 古林真衣子, 梅野太輔, 代謝ネットワーク建設のための進化分子工学―進化的デザインによる非天然代謝経路の創出―, 植田充美（監修）合成生物工学の隆起 ―有用物質の新たな生産法構築をめざして―, シーエムシー出版 (2012) link
  4. 古林真衣子, 田代洋平, 梅野太輔, 材料科学のための合成生物学, 未来材料, 10, 2-7 (2010) link
  5. 梅野太輔, 古林真衣子, 三沢典彦, 第３章・カロテノイドの生合成, 宮下和夫（監修）カロテノイドの科学と最新応用技術, シーエムシー出版, pp.27-37 (2009) link
  6. 梅野太輔, 田代洋平, 古林真衣子, 細胞のプログラム死と合成生物学. 極限環境微生物学会誌, 7, 31-35 (2008) link

特許

  1. 梅野太輔，古林真衣子，三沢典彦，高市真一「炭素数５０のカロテノイドの製造方法」特願2011-130326 (2011/6/10)
  2. 梅野太輔，生悦住茉友，古林真衣子，方波見彰仁，斎藤恭一「テルペン合成酵素遺伝子のスクリーニング方法」特願 2009‐264294 (2009/11) / 特開2011-125334 (2011/6/30)

受賞

  1. ○古林真衣子，生悦住茉友，高市真一，斎藤恭一，梅野太輔「人工代謝経路によるC50-β-Caroteneの生合成」日本農芸化学会関東支部 2011年度大会，若手奨励賞（口頭発表・学生部門）
  2. iGEM Chiba，千葉大学 平成１８年度課外活動等による学長表彰（コンペ部門）(2007)



べんりリンク

リンク集

ソフト

文献管理/SBM

データベース

Promoter

RBS

レアコドン

たんぱく

イースト

おてがるクローニング

  1. NEBioCalculator
  2. j5
  3. Type IIS
    1. FASTR (LguI) : Kotera I 2008 J Biotechnol / 日本語解説記事（生命化学研究レター）←よりスモールスケール
    2. Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE
    3. Golden Gate Shuffling: Engler C 2009 PLoS ONE
  4. SLIC / Ligation Independent Cloning
    1. Li 2007 Nat Method / EMBL / BitesizeBio (T4 DNA pol)
    2. One step SLIC
    3. In-Fusion (poxvirus DNA pol)
  5. Gibson
  6. PCRベース
    1. MEGAWHOP library cloning 2011 2003 2002
    2. Overlap extension PCR
    3. Circular Polymerase Extension Cloning (CPEC)
  7. リコンビニアリング
    1. yeast: Shanks RMQ 2009 Plasmid
    2. RecET cloning: Fu 2009 Nat Biotechnol
    3. lambda RED on RK2

実験tips

HPLC/GC

撮影

かきもの

スタイルガイド

論文・文章の書き方

Scrivener

ワード

マークアップ／プログラミング

ず

日本語

英語

日本語と英語

フェローシップ

よみもの

すばらしい

サイエンス

Origin of stuff

ヒューマン

教科書

ラボライフハック

Procrastination



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