User:Maiko Furubayashi/jp

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(おてがるクローニング)
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==おてがるクローニング==
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==おてがるクローニング / super easy cloning==
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#ligation independent cloning (LIC) by T4 dnapol
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#[http://www.nature.com/nmeth/journal/v4/n3/abs/nmeth1010.html sequence and ligation independent cloning (SLIC) using T4 DNApol: Li MZ 2007 Nat Meth]
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##[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC332407/ LIC: C Aslanidis 1990 NAR]
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#*[http://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/lic/ EMBLによるプロトコル]
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##[http://www.nature.com/nmeth/journal/v4/n3/abs/nmeth1010.html SLIC: Li MZ 2007 Nat Meth]
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#*[http://bitesizebio.com/articles/sequence-and-ligation-independent-cloning/ BitesizeBioの記事(口コミあり)]
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##*[http://www.embl.de/pepcore/pepcore_services/cloning/cloning_methods/lic/ EMBLによるプロトコル]
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#ligation independent cloning by PCR
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##*[http://bitesizebio.com/articles/sequence-and-ligation-independent-cloning/ BitesizeBioの記事(口コミあり)]
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#ligation independent cloning (LIC) by PCR
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##[http://www.biotechniques.com/BiotechniquesJournal/2010/June/Overlap-extension-PCR-cloning-a-simple-and-reliable-way-to-create-recombinant-plasmids/biotechniques-280116.html Overlap extension PCR cloning: Bryksin AV 2010 BioTechniques]
##[http://www.biotechniques.com/BiotechniquesJournal/2010/June/Overlap-extension-PCR-cloning-a-simple-and-reliable-way-to-create-recombinant-plasmids/biotechniques-280116.html Overlap extension PCR cloning: Bryksin AV 2010 BioTechniques]
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##[http://www.biotechniques.com/BiotechniquesJournal/2011/July/Self-assembly-cloning-a-rapid-construction-method-for-recombinant-molecules----from-multiple-fragments/biotechniques-318362.html Self-assembly cloning: Matsumoto A 2011 BioTechniques]
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##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0006441 Circular Polymerase Extension Cloning: Quan 2009 PLoS ONE]
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#Type IIS 制限酵素を使って同時に digestion & ligation.
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#digest and ligate in one pot using Type IIS restriction enzyme
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#*[http://www.sciencedirect.com/science/article/pii/S0168165608020804 FASTR: Kotera I 2008 J Biotechnol]
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##[http://www.sciencedirect.com/science/article/pii/S0168165608020804 FASTR (LguI) : Kotera I 2008 J Biotechnol]
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##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0003647 Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE]
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##[http://www.plosone.org/article/info:doi%2F10.1371%2Fjournal.pone.0005553 Golden Gate Shuffling: Engler C 2009 PLoS ONE]
#Gibson Assembly
#Gibson Assembly
#*[http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html Gibson DG 2009 Nat Meth]
#*[http://www.nature.com/nmeth/journal/v6/n5/full/nmeth.1318.html Gibson DG 2009 Nat Meth]
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==実験tips==
==実験tips==

Revision as of 11:43, 3 June 2012

English/日本語

古林 真衣子

  • 千葉大学大学院 工学研究科 共生応用化学専攻 博士後期課程1年
  • バイオマテリアル研究室 梅野太輔グループ
  • 日本学術振興会 特別研究員 (DC1)

Contact

  • maiko.furubayashi *at* gmail.com
  • 263-8522 千葉市稲毛区弥生町1-33 千葉大学工学部1号棟313号室


業績

論文・総説

  1. 古林真衣子, 梅野太輔, 代謝ネットワーク建設のための進化分子工学―進化的デザインによる非天然代謝経路の創出―, 植田充美(監修)合成生物工学の隆起 ―有用物質の新たな生産法構築をめざして―, シーエムシー出版 (2012) link
  2. Furubayashi M, Umeno D, Directed Evolution of Carotenoid Synthases for the Production of Unnatural Carotenoids. Methods Mol Biol 892 (2012) link
  3. 古林真衣子, 田代洋平, 梅野太輔, 材料科学のための合成生物学, 未来材料, 10, 2-7 (2010) link
  4. 梅野太輔, 古林真衣子, 三沢典彦, 第3章・カロテノイドの生合成, 宮下和夫(監修)カロテノイドの科学と最新応用技術, シーエムシー出版, pp.27-37 (2009) link
  5. 梅野太輔, 田代洋平, 古林真衣子, 細胞のプログラム死と合成生物学. 極限環境微生物学会誌, 7, 31-35 (2008) link
  6. Tashiro Y, Furubayashi M, Morijiri T, Suzuki K, Yasuno K, Matsuno S, Katabami A, Saito K, Umeno D, Escherichia coli robots that freeze, smell, swell, and time-keep. Synthetic Biology, IET., 1, 41-43 (2007)

特許

  1. 梅野太輔,古林真衣子,三沢典彦,高市真一「炭素数50のカロテノイドの製造方法」特願2011-130326 (2011/6/10)
  2. 梅野太輔,生悦住茉友,古林真衣子,方波見彰仁,斎藤恭一「テルペン合成酵素遺伝子のスクリーニング方法」特願 2009‐264294 (2009/11) / 特開2011-125334 (2011/6/30)

受賞

  1. ○古林真衣子,生悦住茉友,高市真一,斎藤恭一,梅野太輔「人工代謝経路によるC50-β-Caroteneの生合成」日本農芸化学会関東支部 2011年度大会,若手奨励賞(口頭発表・学生部門)
  2. iGEM Chiba,千葉大学 平成18年度課外活動等による学長表彰(コンペ部門)(2007)


べんりリンク

bioinformatics tools

リンク集

たんぱく

フリーソフト


database

よみもの

文献管理/SBM

Useful tips/links for DNA construct design

Promoter

RBS


おてがるクローニング / super easy cloning

  1. sequence and ligation independent cloning (SLIC) using T4 DNApol: Li MZ 2007 Nat Meth
  2. ligation independent cloning by PCR
    1. Overlap extension PCR cloning: Bryksin AV 2010 BioTechniques
    2. Circular Polymerase Extension Cloning: Quan 2009 PLoS ONE
  3. digest and ligate in one pot using Type IIS restriction enzyme
    1. FASTR (LguI) : Kotera I 2008 J Biotechnol
    2. Golden Gate Cloning (BsaI): Engler C 2008 PLoS ONE
    3. Golden Gate Shuffling: Engler C 2009 PLoS ONE
  4. Gibson Assembly

実験tips


ほか

Personal tools