User:Marcus McHale

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=Marcus McHale=
==Contact Info==
==Contact Info==
[[Image:Marcus_mchale_big.jpg|thumb|right|Marcus McHale]]
[[Image:Marcus_mchale_big.jpg|thumb|right|Marcus McHale]]
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*[http://www.bio.usyd.edu.au/Social_InsectsLab/Social_InsectsLab.htm Behaviour and Genetics of Social Insects Laboratory]
 
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*[http://www.bio.usyd.edu.au/ School of Biological Sciences]
 
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*Macleay Building, A12
 
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*[http://www.usyd.edu.au/ University of Sydney]
 
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*NSW, 2006,
 
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*AUSTRALIA
 
*[[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]]
*[[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]]
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==Projects==
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===Current===
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====Waterhouse Laboratory (PhD)====
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http://sydney.edu.au/science/molecular_bioscience/waterhouse/
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* Transgene susceptibility to silencing in plants
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===Past===
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====Behaviour and Genetics of Social Insects Laboratory====
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Research Assistant/Lab Manager
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*Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR
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*Assisting students with their projects
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====Department of Primary Industry and Fisheries, Queensland====
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=====Molecular Fisheries Laboratory=====
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======Dugong (Research assistant)======
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*Gender assignment (sexing) of dugong tissue biopsies
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* Amplification of molecular markers from faeces (only succeeded in detecting mitochondrial sequences)
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* Population genetics of dugong in and around Moreton Bay (Brisbane, Australia), I found evidence in mitochondrial DNA sequences of migrant populations from Harvey bay consistent with a cyclone event and mass extinction observed there.
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======Spanish mackerel (Research technician)======
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* Genetic mark-recapture of Spanish mackerel (Scomberomorus commerson) and dugong (Dugong dugon) [http://www.nt.gov.au/dpifm/Fisheries/index.cfm?header=Genetag%20Research%20Project NT DPI Genetag][http://www.dpi.qld.gov.au/cps/rde/dpi/hs.xsl/28_10203_ENA_HTML.htm|QLD DPI Dugong]
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=====Emerging Technologies (Honours and Undergraduate volunteer work)=====
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* Thesis:  Durable RNA Silencing Mediated Transgenic Virus Resistance in Plants
==Education==
==Education==
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*2009 - Current PhD Candidate, [http://www.bio.usyd.edu.au/ School of Biological Sciences]  [http://www.usyd.edu.au/ University of Sydney]]
*2005 - Honours (class I) from the [http://www.smms.uq.edu.au School of Molecular and Microbial Sciences] at the [http://www.uq.edu.au University of Queensland]
*2005 - Honours (class I) from the [http://www.smms.uq.edu.au School of Molecular and Microbial Sciences] at the [http://www.uq.edu.au University of Queensland]
*2004 - Bachelor of Science (Botany and Genetics) from the [http://www.uq.edu.au University of Queensland]
*2004 - Bachelor of Science (Botany and Genetics) from the [http://www.uq.edu.au University of Queensland]
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==Research interests==
 
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* Development and application of molecular technologies
 
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* Epigenetics and gene regulation (particularly small RNA mediated)
 
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* Agricultural biodiversity
 
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* Fisheries depletion
 
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==Projects==
 
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===Current===
 
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* Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR  [http://www.bio.usyd.edu.au/Social_InsectsLab/BensPDFs/Oldroyd_and_Thompson.pdf]
 
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===Past===
 
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* Gender assignment (sexing) of dugong tissue biopsies [http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1471-8286.2007.02041.x]
 
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* Genetic mark-recapture of Spanish mackerel (Scomberomorus commerson) and dugong (Dugong dugon) [http://www.nt.gov.au/dpifm/Fisheries/index.cfm?header=Genetag%20Research%20Project][http://www.dpi.qld.gov.au/cps/rde/dpi/hs.xsl/28_10203_ENA_HTML.htm]
 
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* Durable RNA silencing mediated transgenic virus resistance in plants [http://www.publish.csiro.au/paper/AP06064.htm]
 
==Publications==
==Publications==
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#[http://www.blackwell-synergy.com/doi/pdf/10.1111/j.1471-8286.2007.02041.x A multiplex PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON. Molecular Ecology Resources (2008) 8, 669–670]
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*A PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON
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*A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea.  Jessica S. Higgs, Marcus McHale and Benjamin P. Oldroyd.  Apidologie 41 (2010) 96–98
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*Expression of genes related to reproduction and pollen foraging in honey bees (Apis mellifera) narcotized with carbon dioxide. R. M. Brito, M. McHale and B. P. Oldroyd. Insect Molecular Biology (2010) 19(4), 451–461
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====Bookmarks====
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*[http://gentle.magnusmanske.de GENtle, open source software for cloning - I wish it got more attention]
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*[http://www.rdml.org/guidelines.php MIQE Guidelines] an effort to standardise reporting of quantitative PCR experiments.
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*[http://staden.sourceforge.net/ Staden Package] Powerful tool for sequence allignment and quality checking traces.
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*[http://okeylabimac.med.utoronto.ca/PUNS/ Primer-UniGene Selectivity Testing] Useful for PCR development in genome sequenced species.
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*[http://www.arabidopsis.org/ The Arabidopsis Information Resource]
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*[http://epigenomics.mcdb.ucla.edu/ UCSC genome browser and other resources]
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[[Category:User Australia]]
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== OpenWetWare ==
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==OpenWetWare==
*[[Special:Contributions/Marcus McHale | My Contributions]]
*[[Special:Contributions/Marcus McHale | My Contributions]]
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*[[DNA_extraction_-_Salting_Out]]
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*[[DNA extraction - Salting Out]]
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==Bookmarks==
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*[http://www.ubcbotanicalgarden.org/potd/ Botany Photo of the Day] Plant Porn
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*[http://okeylabimac.med.utoronto.ca/PUNS/ Primer-UniGene Selectivity Testing] This is incredibly useful now that I work on a well studied species (''Apis mellifera'')
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[[Category:User Australia]]
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Current revision

Contents

Marcus McHale

Contact Info

Marcus McHale
Marcus McHale

Projects

Current

Waterhouse Laboratory (PhD)

http://sydney.edu.au/science/molecular_bioscience/waterhouse/

  • Transgene susceptibility to silencing in plants

Past

Behaviour and Genetics of Social Insects Laboratory

Research Assistant/Lab Manager

  • Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR
  • Assisting students with their projects

Department of Primary Industry and Fisheries, Queensland

Molecular Fisheries Laboratory
Dugong (Research assistant)
  • Gender assignment (sexing) of dugong tissue biopsies
  • Amplification of molecular markers from faeces (only succeeded in detecting mitochondrial sequences)
  • Population genetics of dugong in and around Moreton Bay (Brisbane, Australia), I found evidence in mitochondrial DNA sequences of migrant populations from Harvey bay consistent with a cyclone event and mass extinction observed there.
Spanish mackerel (Research technician)
  • Genetic mark-recapture of Spanish mackerel (Scomberomorus commerson) and dugong (Dugong dugon) NT DPI GenetagDPI Dugong
Emerging Technologies (Honours and Undergraduate volunteer work)
  • Thesis: Durable RNA Silencing Mediated Transgenic Virus Resistance in Plants

Education

Publications

  • A PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON
  • A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea. Jessica S. Higgs, Marcus McHale and Benjamin P. Oldroyd. Apidologie 41 (2010) 96–98
  • Expression of genes related to reproduction and pollen foraging in honey bees (Apis mellifera) narcotized with carbon dioxide. R. M. Brito, M. McHale and B. P. Oldroyd. Insect Molecular Biology (2010) 19(4), 451–461

Bookmarks

OpenWetWare

Personal tools