User:Marcus McHale: Difference between revisions

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=Marcus McHale=
==Contact Info==
==Contact Info==
[[Image:Marcus_mchale_big.jpg|thumb|right|Marcus McHale]]
[[Image:Marcus_mchale_big.jpg|thumb|right|Marcus McHale]]
*[[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]]
*[[Special:Emailuser/Marcus McHale|Email me through OpenWetWare]]
==Education==
*2009 - Current PhD Candidate, [http://www.bio.usyd.edu.au/ School of Biological Sciences], [http://www.usyd.edu.au/ University of Sydney]
*2005 - Honours (class I) from the [http://www.smms.uq.edu.au School of Molecular and Microbial Sciences] at the [http://www.uq.edu.au University of Queensland]
*2004 - Bachelor of Science (Botany and Genetics) from the [http://www.uq.edu.au University of Queensland]
==Research interests==
* Epigenetics and Gene Silencing
* Agricultural biodiversity
* Fisheries depletion
==Projects==
==Projects==
===Current===
===Current===
* Investigating transitive gene silencing of endogenes in plants [http://www.pi.csiro.au/RNAi/aboutus.htm#waterhouse]
====Waterhouse Laboratory (PhD)====
 
http://sydney.edu.au/science/molecular_bioscience/waterhouse/
* Transgene susceptibility to silencing in plants
===Past===
===Past===
* Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR [http://www.bio.usyd.edu.au/Social_InsectsLab/BensPDFs/Oldroyd_and_Thompson.pdf]
====Behaviour and Genetics of Social Insects Laboratory====
* Gender assignment (sexing) of dugong tissue biopsies [http://www.dpi.qld.gov.au/cps/rde/xbcr/dpi/Dugong-research-McHale-et-al-2008.pdf]
Research Assistant/Lab Manager
* Population genetics of Dugong in and around Moreton Bay (Brisbane, Australia)
*Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR
* Genetic mark-recapture of Spanish mackerel (Scomberomorus commerson) and dugong (Dugong dugon) [http://www.nt.gov.au/dpifm/Fisheries/index.cfm?header=Genetag%20Research%20Project][http://www.dpi.qld.gov.au/cps/rde/dpi/hs.xsl/28_10203_ENA_HTML.htm]
*Assisting students with their projects
* Durable RNA silencing mediated transgenic virus resistance in plants (Honours project)[http://www.publish.csiro.au/paper/AP06064.htm]


==Publication==
====Department of Primary Industry and Fisheries, Queensland====
*A PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON. [http://www3.interscience.wiley.com/journal/120090724/abstract]
=====Molecular Fisheries Laboratory=====
======Dugong (Research assistant)======
*Gender assignment (sexing) of dugong tissue biopsies
* Amplification of molecular markers from faeces (only succeeded in detecting mitochondrial sequences)
* Population genetics of dugong in and around Moreton Bay (Brisbane, Australia), I found evidence in mitochondrial DNA sequences of migrant populations from Harvey bay consistent with a cyclone event and mass extinction observed there.
======Spanish mackerel (Research technician)======
* Genetic mark-recapture of Spanish mackerel (Scomberomorus commerson) and dugong (Dugong dugon) [http://www.nt.gov.au/dpifm/Fisheries/index.cfm?header=Genetag%20Research%20Project NT DPI Genetag][http://www.dpi.qld.gov.au/cps/rde/dpi/hs.xsl/28_10203_ENA_HTML.htm|QLD DPI Dugong]
=====Emerging Technologies (Honours and Undergraduate volunteer work)=====
* Thesis:  Durable RNA Silencing Mediated Transgenic Virus Resistance in Plants


== OpenWetWare ==
==Education==
*[[Special:Contributions/Marcus McHale | My Contributions]]
*2009 - Current PhD Candidate, [http://www.bio.usyd.edu.au/ School of Biological Sciences]  [http://www.usyd.edu.au/ University of Sydney]]
*[[DNA extraction - Salting Out]]
*2005 - Honours (class I) from the [http://www.smms.uq.edu.au School of Molecular and Microbial Sciences] at the [http://www.uq.edu.au University of Queensland]
*2004 - Bachelor of Science (Botany and Genetics) from the [http://www.uq.edu.au University of Queensland]


==Bookmarks==
==Publications==
*A PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON
*A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea.  Jessica S. Higgs, Marcus McHale and Benjamin P. Oldroyd.  Apidologie 41 (2010) 96–98
*Expression of genes related to reproduction and pollen foraging in honey bees (Apis mellifera) narcotized with carbon dioxide. R. M. Brito, M. McHale and B. P. Oldroyd. Insect Molecular Biology (2010) 19(4), 451–461
====Bookmarks====
*[http://gentle.magnusmanske.de GENtle, open source software for cloning - I wish it got more attention]
*[http://www.rdml.org/guidelines.php MIQE Guidelines] an effort to standardise reporting of quantitative PCR experiments.
*[http://www.rdml.org/guidelines.php MIQE Guidelines] an effort to standardise reporting of quantitative PCR experiments.
*[http://staden.sourceforge.net/ Staden Package] Powerful tool for sequence allignment and quality checking traces.
*[http://staden.sourceforge.net/ Staden Package] Powerful tool for sequence allignment and quality checking traces.
*[http://okeylabimac.med.utoronto.ca/PUNS/ Primer-UniGene Selectivity Testing] Useful for PCR development in genome sequenced species.
*[http://okeylabimac.med.utoronto.ca/PUNS/ Primer-UniGene Selectivity Testing] Useful for PCR development in genome sequenced species.
*[http://www.arabidopsis.org/ The Arabidopsis Information Resource]
*[http://epigenomics.mcdb.ucla.edu/ UCSC genome browser and other resources]
[[Category:User Australia]]
[[Category:User Australia]]
==OpenWetWare==
*[[Special:Contributions/Marcus McHale | My Contributions]]
*[[DNA extraction - Salting Out]]

Latest revision as of 18:56, 14 February 2011

Marcus McHale

Contact Info

Marcus McHale

Projects

Current

Waterhouse Laboratory (PhD)

http://sydney.edu.au/science/molecular_bioscience/waterhouse/

  • Transgene susceptibility to silencing in plants

Past

Behaviour and Genetics of Social Insects Laboratory

Research Assistant/Lab Manager

  • Investigating the expression of genes related to ovary activation in honey bees (Apis mellifera) using real-time PCR
  • Assisting students with their projects

Department of Primary Industry and Fisheries, Queensland

Molecular Fisheries Laboratory
Dugong (Research assistant)
  • Gender assignment (sexing) of dugong tissue biopsies
  • Amplification of molecular markers from faeces (only succeeded in detecting mitochondrial sequences)
  • Population genetics of dugong in and around Moreton Bay (Brisbane, Australia), I found evidence in mitochondrial DNA sequences of migrant populations from Harvey bay consistent with a cyclone event and mass extinction observed there.
Spanish mackerel (Research technician)
Emerging Technologies (Honours and Undergraduate volunteer work)
  • Thesis: Durable RNA Silencing Mediated Transgenic Virus Resistance in Plants

Education

Publications

  • A PCR assay for gender assignment in dugong (Dugong dugon) and West-Indian manatee (Trichechus manatus). M. MCHALE, D. BRODERICK, J . R. OVENDEN and J . M. LANYON
  • A scientific note on a rapid method for the molecular discrimination of Apis andreniformis and A. florea. Jessica S. Higgs, Marcus McHale and Benjamin P. Oldroyd. Apidologie 41 (2010) 96–98
  • Expression of genes related to reproduction and pollen foraging in honey bees (Apis mellifera) narcotized with carbon dioxide. R. M. Brito, M. McHale and B. P. Oldroyd. Insect Molecular Biology (2010) 19(4), 451–461

Bookmarks

OpenWetWare

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