User:Maria Briscione/Notebook/Chem 571/2011/09/20

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Objective

Description

Forward and reverse PCR primers were determined in accordance to the guidelines set forth by the Quick Change Manual. Then a PCR reaction was carried out using previously prepared primers. First, 43 μL of ddH2O was added. Then 1 μL on a 0.5 μ/μL of template DNA, 1 μL of a 10mM dNTP solution, and 1.25 μL of a 100 ng/μL solution of forward and reverse primers was pipetting into the solution. Finally, immediately before the solution was put into a thermocycler 1 μL of enzymes were added to the solution and 25 μL of wax was added slowly to the surface of the solution.

Data

Temperature Cycling:


Primers:

  • FORWARD: 5' GAC GAT GAC GAT AAG GAT CGA TGG GGA TCC GAA 3'
  • REVERSE 5' TTC GGA TCC CCA CCA TCG ATC CTT ATC GTC ATC GTC 3'

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.



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