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Objective
Description
Microwave Assisted Acid Base Hydrolysis
- determine how much BSA is used in AuNP
- pull apart and cut into individual AA
- will examine spectra of tryptophan- look around 280
Modified Procedure
- 100 µL AuNP
- 100 µL 6 M HCl - until colorless
- put 200 µL 4 M NaOH
- 100 µL solution and put in pipette tube
- 2 µL DTT
- stick in microwave next to flask of water a microwave for 2 min
- UV vis
- blank- use 100 µL of water instead of AuNP to make blank
- repeat for 2 different concentrations of BSA
- tried adding 100 µL 4 M NaOH + 100 µL of AuNP in plastic pipette tube
- glass 50 µL 6 M HCl + 100 µL AuNP in glass
Original Procedure
- 40 µL of 1 mg/ml BSA in water with 2 µL of 450 mM DTT
- Add 40 µL of 2 M NaOH in a 1.5 ml polypropylene vial
- Capped and placed in microwave 1000 W Power
- With excess energy being absorbed by 100 ml of water in loosely covered container beside
- Microwave sample for 30-30 s and UV-Vis was measured
- put acid in first
Data
- Add data and results here...
Notes
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Biomaterials Design Lab
