User:Maria Clara T Astolfi: Difference between revisions

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==Contact me! Say Hello!==
==Contact me! Say Hello!==
{{wide image|Mctatolfi 16S rRNA.jpg|1800px|center|}}
[[Image:Maria Astolfi photo.jpg|upright=1.5|thumb|right|Maria Clara T Astolfi]]
[[Image:Maria Astolfi photo.jpg|upright=1.5|thumb|right|Maria Clara T Astolfi]]


*Maria Clara T Astolfi
*Maria Clara T Astolfi
*Federal University of Amazonas
*Biotechnology student at Federal University of Amazonas
*Synthetic Biology Hub of Amazonas
*Instructor AND co-founder
*Synthetic Biology Hub of Amazonas  
*Multidisciplinary Support Centre   
*Multidisciplinary Support Centre   
*Manaus, Amazonas - Brazil  
*Manaus, Amazonas - Brazil  
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*[[Special:Emailuser/Maria Clara T Astolfi|Email me through OpenWetWare]] or use mctastolfi@gmail.com
*[[Special:Emailuser/Maria Clara T Astolfi|Email me through OpenWetWare]] or use mctastolfi@gmail.com


I work in the [[Your Lab]] at XYZ University. I learned about [[OpenWetWare]] from iGEM competition! I've loved the idea since we founded the Synthetic Biology Hub of Amazonas - Brazil in 2012. Currently, I'm an instructor of our iGEM team (Amazonas_Brazil), bringing up new SynBio students, highlighting BioBricks Foundation philosophy and goals,  building new genetic circuits. Our aim is to contribute to the SynBio community and remove barriers in the way of its advancement! In 2014, we constructed several standards BioBricks for mercury bioremediation and novel promoters for its improvement. Our hub has also worked building a new logic gate to construct promoters standardizing different sigma factors, operators, -35, -10, extended -10, discriminator and more. This experience inspired us to found the '''Genetic Expression and Regulation Group''' and now we are paving the way to work with riboswitches, RNAi and other technologies. Now we are working to build a standard BioBrick vector for CRISPR/Cas9 genome engineering in E. coli: the CRISPeasy!
I learned about [[OpenWetWare]] from iGEM competition! I work at the Synthetic Biology Hub of Amazonas since its foundation and first iGEM participation in 2013, when I was a first-year high school student. Currently, I'm an instructor of our iGEM team (Amazonas_Brazil) and hub, bringing up new SynBio passionate students, highlighting and learning together the foundations for engineer biology. Our aim is to contribute to the SynBio community and remove boundaries in the way of its advancement in the middle of Amazon rainforest - Brazil! In 2014, we engineered a standard BioBrick for mercury processes (e.g., biosensor, biosorption and bioremediation). In 2015-2016, we constructed novel synthetic promoters expanding the logic of its recognition that amplified mercury bioremediation in genetically engineered machines - unifying amazonian challenges with SynBio approach! Now we are working to build a standard BioBrick vector for one-step bacterial genome engineering with CRISPR/Cas9 machinery: the CRISPeasy!


'''I would love to share our results and what we have done since 2012!'''
'''I would love to share our results and what we have done since 2013!'''
 
==News from our Lab==
 
We are proud to present: '''the pDM02 promoter!''' It's a novel promoter from our laboratory, designed by Diego and Maria, which the lac operator overlaps the -35 region. We achieved an awesome regulation with this new approach. When adding glucose (left side), the GFP expression is completely repressed.
 
[[Image:Mariastolfi pDM02 promoter.jpg|thumb|center|upright=2|alt=A New regulated promoter with lac operator overlapped to -35 region.|Our novel promoter! Say hello to pDM02!]]
 
More information will be available soon!


==Education==
==Education==
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==Research interests==
==Research interests==
<!-- Feel free to add brief descriptions to your research interests as well -->
<!-- Feel free to add brief descriptions to your research interests as well -->
# Synthetic Biology
# Rationally design biology to overcome synthetic biology boundaries
# Synthetic promoters
# Engineer novel regulatory mechanisms AND genetic circuits
# Gene expression and regulation applied to build new tools in genetic circuits
# Transform complexity into abstraction, inputs into predictable outputs


==Useful links==
==Useful links==
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[OpenWetWare:Welcome|Introductory tutorial]]
*[[Help|OpenWetWare help pages]]
*[[Help|OpenWetWare help pages]]

Latest revision as of 11:25, 3 May 2018

Contact me! Say Hello!

Maria Clara T Astolfi
  • Maria Clara T Astolfi
  • Biotechnology student at Federal University of Amazonas
  • Instructor AND co-founder
  • Synthetic Biology Hub of Amazonas
  • Multidisciplinary Support Centre
  • Manaus, Amazonas - Brazil

I learned about OpenWetWare from iGEM competition! I work at the Synthetic Biology Hub of Amazonas since its foundation and first iGEM participation in 2013, when I was a first-year high school student. Currently, I'm an instructor of our iGEM team (Amazonas_Brazil) and hub, bringing up new SynBio passionate students, highlighting and learning together the foundations for engineer biology. Our aim is to contribute to the SynBio community and remove boundaries in the way of its advancement in the middle of Amazon rainforest - Brazil! In 2014, we engineered a standard BioBrick for mercury processes (e.g., biosensor, biosorption and bioremediation). In 2015-2016, we constructed novel synthetic promoters expanding the logic of its recognition that amplified mercury bioremediation in genetically engineered machines - unifying amazonian challenges with SynBio approach! Now we are working to build a standard BioBrick vector for one-step bacterial genome engineering with CRISPR/Cas9 machinery: the CRISPeasy!

I would love to share our results and what we have done since 2013!

Education

  • Biotechnology, Federal University of Amazonas

Research interests

  1. Rationally design biology to overcome synthetic biology boundaries
  2. Engineer novel regulatory mechanisms AND genetic circuits
  3. Transform complexity into abstraction, inputs into predictable outputs

Useful links