User:Maria Clara T Astolfi: Difference between revisions

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We are proud to present: '''the pDM02 promoter!''' It's a novel promoter from our laboratory, designed by Diego and Maria, which the lac operator overlaps the -35 region. We achieved an awesome regulation with this new approach. When adding glucose, the GFP expression is completely repressed.  
We are proud to present: '''the pDM02 promoter!''' It's a novel promoter from our laboratory, designed by Diego and Maria, which the lac operator overlaps the -35 region. We achieved an awesome regulation with this new approach. When adding glucose, the GFP expression is completely repressed.  


[[Image:Mariastolfi pDM02 promoter.jpg|thumb|center|alt=A New regulated promoter with lac operator overlapped to -35 region.|Our novel promoter! Say hello for pDM02.]]  
[[Image:Mariastolfi pDM02 promoter.jpg|thumb|center|upright=1.35|alt=A New regulated promoter with lac operator overlapped to -35 region.|Our novel promoter! Say hello for pDM02.]]  


More information will be available soon!
More information will be available soon!

Revision as of 14:00, 22 June 2017

I am a new member of OpenWetWare!

Contact me! Say Hello!

Maria Clara T Astolfi (an artistic interpretation)
  • Maria Clara T Astolfi
  • Federal University of Amazonas
  • Synthetic Biology Hub of Amazonas
  • Multidisciplinary Support Centre
  • Manaus, Amazonas - Brazil

I work in the Your Lab at XYZ University. I learned about OpenWetWare from iGEM competition! I've loved the idea since we founded the Synthetic Biology Hub of Amazonas - Brazil in 2012. Currently, I'm an instructor of our iGEM team (Amazonas_Brazil), bringing up new SynBio students, highlighting BioBricks Foundation philosophy and goals, building new genetic circuits. Our aim is to contribute to the SynBio community and remove barriers in the way of its advancement! In 2014, we constructed several standards BioBricks for mercury bioremediation and novel promoters for its improvement. Our hub has also worked building a new logic gate to construct promoters standardizing different sigma factors, operators, -35, -10, extended -10, discriminator and more. Now we are working to build a standard BioBrick vector for CRISPR/Cas9 genome editing in E. coli, the CRISPeasy.

I would love to share our results and what we have done since 2012!

News from our Lab

We are proud to present: the pDM02 promoter! It's a novel promoter from our laboratory, designed by Diego and Maria, which the lac operator overlaps the -35 region. We achieved an awesome regulation with this new approach. When adding glucose, the GFP expression is completely repressed.

A New regulated promoter with lac operator overlapped to -35 region.
Our novel promoter! Say hello for pDM02.

More information will be available soon!

Education

  • Biotechnology, Federal University of Amazonas

Research interests

  1. Interest 1
  2. Interest 2
  3. Interest 3

Publications

  1. Goldbeter A and Koshland DE Jr. An amplified sensitivity arising from covalent modification in biological systems. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6840-4. DOI:10.1073/pnas.78.11.6840 | PubMed ID:6947258 | HubMed [Paper1]
  2. JACOB F and MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318-56. DOI:10.1016/s0022-2836(61)80072-7 | PubMed ID:13718526 | HubMed [Paper2]

    leave a comment about a paper here

  3. ISBN:0879697164 [Book1]

All Medline abstracts: PubMed | HubMed

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