User:Maria Clara T Astolfi
I am a new member of OpenWetWare!
Contact me! Say Hello!
- Maria Clara T Astolfi
- Federal University of Amazonas
- Synthetic Biology Hub of Amazonas
- Multidisciplinary Support Centre
- Manaus, Amazonas - Brazil
- Email me through OpenWetWare or use mctastolfi@gmail.com
I work in the Your Lab at XYZ University. I learned about OpenWetWare from iGEM competition! I've loved the idea since we founded the Synthetic Biology Hub of Amazonas - Brazil in 2012. Currently, I'm an instructor of our iGEM team (Amazonas_Brazil), bringing up new SynBio students, highlighting BioBricks Foundation philosophy and goals, building new genetic circuits. Our aim is to contribute to the SynBio community and remove barriers in the way of its advancement! In 2014, we constructed several standards BioBricks for mercury bioremediation and novel promoters for its improvement. Our hub has also worked building a new logic gate to construct promoters standardizing different sigma factors, operators, -35, -10, extended -10, discriminator and more. This experience inspired us to found the Genetic Expression and Regulation Group and now we are paving the way to work with riboswitches, RNAi and other technologies. Now we are working to build a standard BioBrick vector for CRISPR/Cas9 genome engineering in E. coli: the CRISPeasy!
I would love to share our results and what we have done since 2012!
News from our Lab
We are proud to present: the pDM02 promoter! It's a novel promoter from our laboratory, designed by Diego and Maria, which the lac operator overlaps the -35 region. We achieved an awesome regulation with this new approach. When adding glucose (left side), the GFP expression is completely repressed.
More information will be available soon!
Education
- Biotechnology, Federal University of Amazonas
Research interests
- Synthetic Biology
- Synthetic promoters
- Gene expression and regulation applied to build new tools in genetic circuits
