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'''1.21.15--Identifying Algae and Protists and Preparing and Plating Serial Dilutions'''
'''1.21.15--Identifying Algae and Protists from Different Niches of the Transect'''


*<u>Purpose</u>:  
*<u>Purpose</u>:  
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-Pipette  
-Pipette  


1. Make a wet mount of  
 
1. Make six wet mount of organisms from the two niches: 3 from the top niche and 3 from the bottom niche.
 
2. Observe organism at objectives 4X, 10X, and 40X.
 
3. Use dichotomous key to identify organism.
 


*<u>Observations and Data</u>:  
*<u>Observations and Data</u>:  
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Note: Organism 1 to 3 are from the bottom niche of the jar. Organisms 4 to 6 are from the top niche of the jar.  
Note: Organism 1 to 3 are from the bottom niche of the jar. Organisms 4 to 6 are from the top niche of the jar.  


First organism-Transparent, one long whip-like flagella, single cell motile, ~60 um
First organism-Transparent, one long whip-like flagella, single cell motile, ~60 um
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*<u>Conclusions and Future Directions</u>  
*<u>Conclusions and Future Directions</u>  


Faults: My group was uncertain if we identified our six organisms correctly. And it was difficult to take picture of Figure 3 and is not a clear picture of that organism. Based off of my previous hypothesis, there is more ____than ____
My group was uncertain if we identified our six organisms correctly. And it was difficult to take a picture of Figure 3 and is not a clear picture of that organism. With further examination, it looks like the second and fifth organisms are the same organism, Gonium. The third organism may have been identified incorrectly as algae, since my group assumed it looked like algae. My hypothesis has been disproved because there was more bacteria than protists in my transect, furthermore, only two out of six organisms were identified as protists and the other four were bacteria.
The third organism we assumed it was algae
With further examination it looks like the second and fifth organisms are the same organism, Gonium.  
 


*<u>Citations</u>:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.
*<u>Citations</u>:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.

Revision as of 22:00, 29 January 2015

1.21.15--Identifying Algae and Protists from Different Niches of the Transect

  • Purpose:

For this lab, the purpose was to identify six organisms in our Hay Infusion culture by using the dichotomous key. My hypothesis from the previous lab was that my transect will be more abundant with protists than algae. During this lab, I will continue to test this hypothesis.

  • Materials and Methods:

-Prepared slides of Hay Infusion Culture from the bottom and top niches.

-Microscope

-Dichotomous Key

-Pipette


1. Make six wet mount of organisms from the two niches: 3 from the top niche and 3 from the bottom niche.

2. Observe organism at objectives 4X, 10X, and 40X.

3. Use dichotomous key to identify organism.


  • Observations and Data:

Hay Infusion Culture Description-

  • smell-->musky, foul, rotten
  • mold on top
  • looks murky and green
  • water tinged brown
  • Descriptions of Organisms and Identification-

Note: Organism 1 to 3 are from the bottom niche of the jar. Organisms 4 to 6 are from the top niche of the jar.


First organism-Transparent, one long whip-like flagella, single cell motile, ~60 um

Second organism-kidney-bean shaped, bubble looking cells inside, spins

Third organism-seaweed looking

Fourth organism-almost circular, white ovals moving inside, colorless

Fifth organism-packman like face, spins, colorless

Sixth organism-peapod shape, long and thin


Figure 1-[[Image:

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Figure 2-[[Image:

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Figure 3-[[Image:

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Figure 4-[[Image:

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Figure 5-[[Image:

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Figure 6-[[Image:

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Figure 1. This is a picture of what we identified as Algae.

Figure 2. This is a picture of what we identified as Gonium.

Figure 3. This is a picture of what we identified as Peranema.

Figure 4. This is a picture of what we identified as Didinium Cyst.

Figure 5. This is a picture of what we identified as Colpidium.

Figure 6. This is a picture of what we identified as Euglena.


  • Conclusions and Future Directions

My group was uncertain if we identified our six organisms correctly. And it was difficult to take a picture of Figure 3 and is not a clear picture of that organism. With further examination, it looks like the second and fifth organisms are the same organism, Gonium. The third organism may have been identified incorrectly as algae, since my group assumed it looked like algae. My hypothesis has been disproved because there was more bacteria than protists in my transect, furthermore, only two out of six organisms were identified as protists and the other four were bacteria.

  • Citations:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.


1.14.15--Biological Life at AU: Observations of a Transect

  • Purpose:

At American University, I am observing Transect 3 (Tall brushes) to understand and identify the organisms that inhabit that area and the abiotic and biotic factors that affect that niche. My hypothesis is that the transect will be more abundant with protists than bacteria. We cannot tell which is more abundant in our transect, an Hay Infusion culture will be made to test this and observations with a microscope need to be made.

  • Materials and Methods:

-Scientific Microscope

-Sample of my transect

-50 mL conical tube

-0.1 gm of dried milk

-Plastic Jar

-500 mLs of deerpark water

-Graduated cylinder

1. I observed and drew a picture of the area of my transect and recorded the abiotic and biotic properties of my transect.

2. I used a 50 mL conical tube to take a soil/vegetation sample of my transect.

3. Back in the lab, my group and I measured 500 mLs of deerpark water with a graduated cylinder. And placed it in the plastic jar.

4. Then, my group and I placed 12 grams of the transect sample and 0.1 gm of dried milk in the plastic jar.

5. We mixed the contents of our Hay Infusion Culture for 10 seconds and removed the lid of the jar.

  • Data and Observations:

Description of Transect-

The abiotic factors:

  • snow
  • dirt
  • water
  • metal post
  • concrete

The biotic factors:

  • leaves
  • bushes
  • grass

Location: inbetween Bender Arena and the Amphitheater

Topography: near steep area

Figure 1-[[Image:

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Figure 2-[[Image:

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Figure 3-[[Image:

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Figure 4-[[Image:

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Figure 1.This is an drawing of our transect (tall brushes) from an aerial view and is labeled with abiotic and biotic factors.

Figure 2.This is a Google image of an aerial view of our transect.

Figure 3.This is an side view of our Hay Infusion culture.

Figure 4.This is an above view of our Hay Infusion culture.

  • Conclusions and Future Directions:

This data from transect does not support or disprove my hypothesis, I am unsure about what specific organism are in my transect. Next lab period, further examination of the incubated Hay Infusion culture will be done.

M.E.

  • Citations:Bentley, M., Walters-Conte, K., and Nancy K. Zeller. 2015. A Laboratory Manual to Accompany: General Biology II. Department of Biology, American University: Washington D.C.
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