User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/04/19: Difference between revisions
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== | ==Designing primers for blue promoter== | ||
* | |||
* La bioparte es: [http://partsregistry.org/Part:BBa_K238013| BBa_K238013] | |||
*Forward: | |||
5’ --> 3´: ''GAATTCGCGGCCGCTTCTAGAG''GTTTTATATGCATTAGCACTAATTGC | |||
Length of 48nt and a GC% of 44%. | |||
*Reverse: | |||
5’ --> 3’: '''CTGCAGCGGCCGCTACTAGTA'''CCTCTGTTAAAAATGTTAATCAATG | |||
Length of 46 nt and a GC% of 43%. | |||
*Italics indicate the prefix, while bold letters indicate the suffix. | |||
Revision as of 09:53, 7 June 2010
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Designing primers for blue promoter
5’ --> 3´: GAATTCGCGGCCGCTTCTAGAGGTTTTATATGCATTAGCACTAATTGC Length of 48nt and a GC% of 44%.
5’ --> 3’: CTGCAGCGGCCGCTACTAGTACCTCTGTTAAAAATGTTAATCAATG Length of 46 nt and a GC% of 43%.
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