User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/04/19: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:Logo_light_minimal_full_res.jpg|150px]] <span style="font-size:22px;"> WiFi coli: A communicolight system</span> | ||
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==Designing primers for blue promoter== | ==Designing primers for blue promoter== | ||
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* The biopart is: [http://partsregistry.org/Part:BBa_K238013 | * The biopart is: [http://partsregistry.org/Part:BBa_K238013 BBa_K238013] designed by KU Leuven team (iGEM 2009).<br /> | ||
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*Forward:<br /> | *Forward:<br /> | ||
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5’ --> 3´: '''''GAATTCGCGGCCGCTTCTAGAG'''''GTTTTATATGCATTAGCACTAATTGC <br /> | 5’ --> 3´: '''''GAATTCGCGGCCGCTTCTAGAG'''''GTTTTATATGCATTAGCACTAATTGC <br /> | ||
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Length of 48nt and a GC% of 44%.<br /> | Length of 48nt and a GC% of 44%.<br /> | ||
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*Reverse:<br /> | *Reverse:<br /> | ||
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5’ --> 3’: '''CTGCAGCGGCCGCTACTAGTA'''CCTCTGTTAAAAATGTTAATCAATG<br /> | 5’ --> 3’: '''CTGCAGCGGCCGCTACTAGTA'''CCTCTGTTAAAAATGTTAATCAATG<br /> | ||
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Length of 46 nt and a GC% of 43%.<br /> | Length of 46 nt and a GC% of 43%.<br /> | ||
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*Bold Italics indicate the prefix, while bold letters indicate the suffix.<br /> | *Bold Italics indicate the prefix, while bold letters indicate the suffix.<br /> | ||
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Revision as of 16:17, 16 September 2010
 WiFi coli: A communicolight system
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Designing primers for blue promoter
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