User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/03

From OpenWetWare
Jump to navigationJump to search
WiFi coli: A communicolight system <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Repeating the transformation


  • Today, I made the transformation of the ligation realized yesterday. The resulting strains were plated in LB agar and left to grow for 8 hrs. The plates with their respective strains are:


1. Ligation of luciferase and double terminator (T)in a LB with kanamycin plate.
2. The ligation control (plasmid 18 already cut with ) in a LB with tetracycline plate.
3. The transformation control (pSB1T3 with an insert of RFP) in a LB with tetracycline plate.
4. Control of competent cells in a LB with tetracycline plate.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Mariana_Ruiz_Velasco_L./Notebook/IGEM_2010/Wet_lab_journal/2010/06/03&oldid=455574"