User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/06/14: Difference between revisions
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* The 30 cycles were programed as follows: | * The 30 cycles were programed as follows: | ||
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- Initialization step: 95°C for 5 min. (only the 1st mix)<br/ > | - Initialization step: 95°C for 5 min. (only the 1st mix)<br/ > | ||
- Hot start: Stop to add the second mix<br/ > | - Hot start: Stop to add the second mix<br/ > | ||
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- Extension/elongation step: 72°C for 1:50 min.<br/ > | - Extension/elongation step: 72°C for 1:50 min.<br/ > | ||
- Final elongation: 72°C for 5:00 min.<br/ > | - Final elongation: 72°C for 5:00 min.<br/ > | ||
- Final hold: 4°C for ∞. | |||
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Revision as of 17:04, 14 June 2010
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PCR day
--> Mix 2 for 20 μL <-- (this reaction will be added after the hot start)
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