User:Mariana Ruiz Velasco L./Notebook/IGEM 2010/Wet lab journal/2010/07/05: Difference between revisions
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-H<sub>2</sub>O ------------> 13μl<br/ > | -H<sub>2</sub>O ------------> 13μl<br/ > | ||
-Buffer 2 ------> 4μl<br/ > | -Buffer 2 -------> 4μl<br/ > | ||
-BSA ------------> 1μl<br/ > | -BSA ------------> 1μl<br/ > | ||
-DNA ------------> 10μl<br/ > | -DNA ------------> 10μl<br/ > | ||
-Enzyme I ----> 1μl<br/ > | -Enzyme I ------> 1μl<br/ > | ||
-Enzyme II ---> 1μl<br/ > | -Enzyme II -----> 1μl<br/ > | ||
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* '''Reaction Mix''' | * '''Reaction Mix''' | ||
-H<sub>2</sub>O | -H<sub>2</sub>O --------------------> 7μL<br/ > | ||
-Buffer ligase | -Buffer ligase ----------> 2μL<br/ > | ||
-Vector (plasmid) -----> 2μL<br/ > | -Vector (plasmid) -----> 2μL<br/ > | ||
-Insert (luciferase) ---> 3μL<br/ > | -Insert (luciferase) ---> 3μL<br/ > | ||
-Insert (double ter.) | -Insert (double ter.) -> 5μL<br/ > | ||
-Ligase | -Ligase -----------------> 1μL<br/ > | ||
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Revision as of 12:04, 26 September 2010
WiFi coli: A communi-colight system | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Ligation and Transformation of luciferase and double terminator
-H2O --------------------> 7μL
Proving that the ligation was done correctly
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