User:Marie-Eve Val

Vibrio cholerae

site-specific recombination-based engineering tool to massively reorganize V. cholerae's genome

Genome rearrangement events occuring within a single colony

Marie-Eve VAL
INSTITUT PASTEUR
Unité "Plasticité du Génome Bactérien"
Département Génomes et Génétique
CNRS URA2171
25 rue du Dr. Roux
75724 PARIS cedex 15
FRANCE
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Research interests

I am a research associate in Didier Mazel's lab at the Institut Pasteur of Paris. I have a great interest in bacterial genome structuration, organization and maintenance ... and a special interest in multipartite genomes. Owing to its bi-chromosomal genome architecture and its importance in public health, Vibrio cholerae, the causative agent of cholera, has become a preferred model to study bacteria with multipartite genomes. My approach is to drastically alter V. cholerae’s genome structure to gain more insight into multipartite genomes. To do so, we developed a site-specific recombination-based engineering tool, which provides us with a powerful means to massively reorganize in principle any prokaryotic genome.

Education

• 2008 - PhD
  Centre de Génétique Moléculaire (Gif-sur-Yvette) , Francois-Xavier Barre's lab
  Study of the system of chromosome dimer resolution in Vibrio cholerae and its implication in the control of the lysogeny of the phage CTX coding for the choleric toxin
• 2003 - Engineering degree in biotechnology and Master in cellular and molecular biology
  ESBS (Strasbourg, Freiburg, Karlsruhe and Basel)
• 1999 - Technician degree in Biotechnology
  LVC (Gif-sur-Yvette)

Professional Training

• Since 2013 - INSERM Research Associate
  Institut Pasteur (Paris) - Didier Mazel's lab
  
• 2009 / 2013 - Post-Doctoral research training
  Institut Pasteur (Paris) - Didier Mazel's lab
  
• 2004 / 2008 - PhD Thesis
  Centre de Génétique Moléculaire (Gif-sur-Yvette) , Francois-Xavier Barre's lab
  
• 2004 - Engineer position
  Génoscope - Consortium National de Recherche en Génomique (Evry) - Génolevures Consortium
  
• 2003 - Master thesis
  Center of Marine Biotechnology, UMBI (Baltimore) , Hal Schreier's lab
  
• 2002 - Engineer training
  IGBMC (Strasbourg) - Olivier POCH's lab
  
• 2001 - Engineer training
  Biozentrum (Basel) - Peter Philippsen's lab
  
• 1999 / 2000 and summer 2002 - Technician position
  AGROGENE(Moissy-Cramayel)
  
• 1999 - Technician training
  Centre de Génétique Moléculaire (Gif-sur-Yvette) ,Fred Boccard's lab
  

Publications

1. Microbioloy-2014 pmid=24308271

   Fuse or die: how to survive the loss of Dam in Vibrio cholerae

2. Val ME, Skovgaard O, Ducos-Galand M, Bland MJ, and Mazel D. Genome engineering in Vibrio cholerae: a feasible approach to address biological issues. PLoS Genet. 2012 Jan;8(1):e1002472. DOI:10.1371/journal.pgen.1002472 |

   Genome Engineering in Vibrio cholerae: A Feasible Approach to Address Biological Issues

3. Das B, Bischerour J, Val ME, and Barre FX. Molecular keys of the tropism of integration of the cholera toxin phage. Proc Natl Acad Sci U S A. 2010 Mar 2;107(9):4377-82. DOI:10.1073/pnas.0910212107 |

   Molecular keys of the tropism of integration of the cholera toxin phage

4. Génolevures Consortium, Souciet JL, Dujon B, Gaillardin C, Johnston M, Baret PV, Cliften P, Sherman DJ, Weissenbach J, Westhof E, Wincker P, Jubin C, Poulain J, Barbe V, Ségurens B, Artiguenave F, Anthouard V, Vacherie B, Val ME, Fulton RS, Minx P, Wilson R, Durrens P, Jean G, Marck C, Martin T, Nikolski M, Rolland T, Seret ML, Casarégola S, Despons L, Fairhead C, Fischer G, Lafontaine I, Leh V, Lemaire M, de Montigny J, Neuvéglise C, Thierry A, Blanc-Lenfle I, Bleykasten C, Diffels J, Fritsch E, Frangeul L, Goëffon A, Jauniaux N, Kachouri-Lafond R, Payen C, Potier S, Pribylova L, Ozanne C, Richard GF, Sacerdot C, Straub ML, and Talla E. Comparative genomics of protoploid Saccharomycetaceae. Genome Res. 2009 Oct;19(10):1696-709. DOI:10.1101/gr.091546.109 |

   Comparative genomics of protoploid Saccharomycetaceae

5. Val ME, Kennedy SP, El Karoui M, Bonné L, Chevalier F, and Barre FX. FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae. PLoS Genet. 2008 Sep 26;4(9):e1000201. DOI:10.1371/journal.pgen.1000201 |

   FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae

6. Val ME, Bouvier M, Campos J, Sherratt D, Cornet F, Mazel D, and Barre FX. The single-stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae. Mol Cell. 2005 Aug 19;19(4):559-66. DOI:10.1016/j.molcel.2005.07.002 |

   The single-stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae

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