User:Marie-Eve Val

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site-specific recombination-based engineering tool to massively reorganize V. cholerae's genome
site-specific recombination-based engineering tool to massively reorganize V. cholerae's genome
Vibrio cholerae
Vibrio cholerae
Vibrio cholerae with serious segregation problems
Vibrio cholerae with serious segregation problems

Marie-Eve VAL
INSTITUT PASTEUR
Unité "Plasticité du Génome Bactérien"
Département Génomes et Génétique
CNRS URA2171
25 rue du Dr. Roux
75724 PARIS cedex 15
FRANCE
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Contents

Research interests

I am a post-doctoral research fellow in Didier Mazel's lab at the Institut Pasteur of Paris. I have a great interest in bacterial genome structuration, organization and maintenance ... and a special interest in multipartite genomes. Owing to its bi-chromosomal genome architecture and its importance in public health, Vibrio cholerae, the causative agent of cholera, has become a preferred model to study bacteria with multipartite genomes. My approach is to drastically alter V. cholerae’s genome structure to gain more insight into multipartite genomes. To do so, I developed a site-specific recombination-based engineering tool, which provides us with a powerful means to massively reorganize in principle any prokaryotic genome. This genetic tool consists in harnessing the λ and HK022 recombination systems to perform a large panel of genome reorganizations. By controlling the location and the orientation of each partner recombination site, we can obtain a large variety of genome rearrangements. The laboratory of Didier Mazel was an ideal place to initiate such a project since they have developed a large set of genetic tools to work in the vibrios and Didier Mazel has substantial knowledge and experience in site-specific recombination, bacterial genetics and genome analysis.

Education

Professional Training

Publications

  1. Das B, Bischerour J, Val ME, and Barre FX. . pmid:20133778. PubMed HubMed [Paper1]
    Molecular keys of the tropism of integration of the cholera toxin phage.

  2. Génolevures Consortium, Souciet JL, Dujon B, Gaillardin C, Johnston M, Baret PV, Cliften P, Sherman DJ, Weissenbach J, Westhof E, Wincker P, Jubin C, Poulain J, Barbe V, Ségurens B, Artiguenave F, Anthouard V, Vacherie B, Val ME, Fulton RS, Minx P, Wilson R, Durrens P, Jean G, Marck C, Martin T, Nikolski M, Rolland T, Seret ML, Casarégola S, Despons L, Fairhead C, Fischer G, Lafontaine I, Leh V, Lemaire M, de Montigny J, Neuvéglise C, Thierry A, Blanc-Lenfle I, Bleykasten C, Diffels J, Fritsch E, Frangeul L, Goëffon A, Jauniaux N, Kachouri-Lafond R, Payen C, Potier S, Pribylova L, Ozanne C, Richard GF, Sacerdot C, Straub ML, and Talla E. . pmid:19525356. PubMed HubMed [Paper2]
    Comparative genomics of protoploid Saccharomycetaceae.

  3. Val ME, Kennedy SP, El Karoui M, Bonné L, Chevalier F, and Barre FX. . pmid:18818731. PubMed HubMed [Paper3]
    FtsK-dependent dimer resolution on multiple chromosomes in the pathogen Vibrio cholerae.

  4. Val ME, Bouvier M, Campos J, Sherratt D, Cornet F, Mazel D, and Barre FX. . pmid:16109379. PubMed HubMed [Paper4]
    The single-stranded genome of phage CTX is the form used for integration into the genome of Vibrio cholerae.

All Medline abstracts: PubMed HubMed

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