User:Marios F. Sardis: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
 
(108 intermediate revisions by 2 users not shown)
Line 1: Line 1:
[[Image:MeLab.jpg|right|frame|Me in the laboratory of Immunology, Univesity of Crete]]
__NOTOC__current status:Studying, '''MS Protein Biotechnology'''
===Contact info===
{|cellspacing="5" cellpadding="10" style="background:#ffffff; width: 750px;"
Sardis Marios Frantzeskos<br/>
|-valign="top"
Department of Biology<br/>
|style="background:#ffffff"|
University of Crete, Greece<br/>  
[[Image:MeLab.jpg|thumb|left|150px]]
e-mail: hartigan.sc@gmail.com
|style="background:#ffffff"|
 
<font size="4">Sardis Marios Frantzeskos</font size><br>
<font size="2">Department of Biology<br>
University of Crete<BR>
Herakleion, Crete<BR>
Greece<br>
 
''hartigan.sc@gmail.com''
</font size>
|}


===Education===
===Education===
[[Image:Uoc.jpg|40px]] [[Image:Cardiff.jpg|40px]]<br/>
[[Image:Uoc.jpg|40px]] [[Image:Cardiff.jpg|40px]]<br/>
2002-2006 B.Sc. in Biology (Molecular Biology and Biotechnology), University of Crete.<br/>
2006-........MS in Protein Biotechnology, Department of Biology,University of Crete<br/>
2001-2002 Certificate of Higher Education, University of Cardiff, Wales, U.K.
2002-2006 BS in Biology (Biomolecular science and Biotechnology), University of Crete.<br/>
2001-2002 Certificate of Higher Education (in Biology), University of Cardiff, Wales, U.K.


===Awards===
2003-2004 Award granted from the National Institute of Studentships of Greece (IKY) for excellent academic performance.<br/>
2003-2004 Studentship granted from the National Institute of Studentships of Greece (IKY) for top academic performance of the year.


===Research experience===
====Research experience====
[[Image:Dark_field_all.jpg|100px|right]]


In 1967, a puzzling observation was made by Calne and his laboratory team, during liver transplantation experiments on pigs. One animal survived for months, after receiving an allograft, without any immunosupression. Later, it was found that the soluble factors, which were secreted by the liver allograft and were responsible for the development of tolerance to the recipient animal, were soluble MHC molecules(1,2). From this point, many laboratories around the world entered the quest, for the elucidation of the immunoregulatory effects of the various soluble forms of MHC molecules. Today, it is widely accepted that body fluids, isolated from healthy individuals contain various amounts of soluble MHC molecules(1,3,4,5,6). Deviations from the concentration of these molecules in the body fluids of healthy individuals, have been recorded in numerous pathological conditions, such as viral encephalitis5, rheumatoid arthritis(7,8,9), pathologic pregnancies(10), asthma(11) and AIDS(5). These results do not clarify whether soluble MHC molecules have a special role in the immune responses, during the course of these diseases. Additional research has confirmed that soluble forms of MHC molecules can have various immunomodulating effects, such as induction of apoptosis of CD4+ T cells(12); activation of CD8+ T cells(13) and down-regulation of NK activity(13). Soluble dimeric MHC class II molecules built on Fcg2a scaffold can cause activation of T cells and switching towards a TH2 response(14). According to another report, soluble dimeric IEk molecules can act as potent activators for T cells, especially in the presence of the CD4 coreceptor(15).
==Past research interests==
The main focus of my undergraduate research project was the isolation of soluble MHC molecules and thecollection of data about their biological function. We have collected protein solutions that have been isolated from the serum of male and pregnant Balb/c mice, which show anti-I-Ad activity in ELISA, antibody binding inhibition and Western blot assays. After the isolation process was complete, I proceeded with functional assays in order to gather information about the biological role of these molecules. When spleen cells from syngeneic mice were treated with the protein solutions, we had isolated, a significant increase in the incorporation of radioactivity was recorded. However, in some assays, allogeneic spleen cells (C3H/HeN) responded also positively to the soluble molecules we had isolated. Spleen cells that were treated with Concanavalin A and soluble MHC molecules increased the incorporation of radioactivity in an additive manner. Furthermore, during one way, Mixed Lymphocyte Reactions when Balb/c spleen cells that responded to inactivated with mitomycin C3H/HeN spleen cells, were treated with solution of soluble MHC II molecules, their responses were significantly boosted. These results led me to investigate whether there is a specific cell population that responds maximally when these soluble molecules are added to the medium. After separating various cell populations from spleens, according to their surface markers I found that cells bearing the CD4 and CD25 markers and adherent cells tend to respond with maximum efficiency to soluble MHC molecules. I decided to focus more to the CD4 receptor. In order to test whether this molecular structure is important for the effects, I tried to interfere it by adding anti-L3T4 monoclonal antibody to the medium. When CD4 was blocked, the spleen cells did not respond to the soluble molecules.
Furthermore, I decided to test whether the soluble molecules I had isolated, could cause any deviations in the production of immunoglobulins from B-cells. I started by causing polyclonal proliferation of B-cells. This was achieved by adding LPS from E.coli (Type-1 thymus independent antigen) to cultures of freshly isolated spleen cells, from Balb/c mice. To continue, I immunized in vitro the spleen cells with DNP-HSA and in some samples i added soluble MHC molecules. The supernatants were screened for IgM level, with ELISAs. Soluble MHC molecules caused a significant decrease in the level of IgM in the supernatants. This effect could be the result of interference of cell-cell interactions or of the profile of cytokines produced by the spleen cells.   


[[MHC class II molecules]]


References
==Current research interests==


[1]van Rood JJ, van Leeuwen A, van Santen MCT: Anti-HL-A2 inhibitor in normal human serum. Nature (1970) Vol. 226(5243):366<br/>
Molecular motors
[2] Jon J. van Rood and Aad van Leeuwen, Soluble HLA Antigens: How It All Started, Human Immunology (1999) Vol. 60:412–413<br/>
Protein translocation mechanisms in E.coli through the SecA-SecYEG system-Institute of Molecular Biology and Biotechnology-Foundation of Research and Technology Hellas (FORTH), Herakleion, Greece <br/>
[3]Francesco Puppo Francesco Indiveri, Marco Scudeletti and Soldano Femme, ., Soluble HLA
[http://www.imbb.forth.gr/people/aeconomou/sec.html'''SecA translocase''']<br/>
antigens: new roles and uses, Trends in Immunology (1997), April (unspecified pages)<br/>
[http://www.imbb.forth.gr/people/aeconomou/'''Ecolab''']<br/>
[4] Masao Hagihara, Tatsuo Shimura, Kozue Yamamoto, Kentaro Takebe, Batmunkh Munkhbat, and Kimiyoshi Tsuji, Soluble HLA Class I and Class II in Japanese, Human Immunology(1994) Vol. 40:
171-173<br/>
[5] Donnie Aultman, Irena Adamashvili, Kamalakar Yaturu, Marlyn Langford, Frank Gelder, Michael Gautreaux, G. E. Ghali, and John McDonald , Soluble HLA in Human Body Fluids, Human Immunology(1999) Vol. 60: 239–244<br/>
[6] Kerstin A. Pfeiffer, Vera Rebmann, Monika Passler, Kathrin van der Ven, Hans van der Ven,
Dieter Krebs, and Hans Grosse-Wilde , Soluble HLA Levels in Early Pregnancy After In Vitro
Fertilization, Human Immunology (2000) Vol. 61: 559–564<br/>
[7] Leon A. Verbruggen, Hendrik Versaen, Vera Rebmann, William Duquet, Seija De Cock, Hans
Grosse-Wilde, and Christian Demanet, Soluble HLA-DR Levels in Serum are Associated With Therapy and Genetic Factors in Rheumatoid Arthritis, Human Immunology (2002) Vol. 63: 758–764<br/>
[8] Wolf RE, Adamashvili IM, Gelder FB, Hall VC, Fraser PA, McDonald JC., Soluble HLA-I in Rheumatic Diseases Human Immunology(1998) Vol. 59: 644–649<br/>  
[9] L.A. Verbruggen, N. Dumarey, H. Van de Velde,V. Rebmann, J. Flament, C. Van Wayenberge, H. Grosse-Wilde, C. Demanet, Soluble HLA-DR antigen levels in serum correlate with rheumatoid arthritis disease activity and the presence of disease-associated epitopes, Tissue Antigens (2000) Vol. 56: 436–440<br/>
[10] Andrea Steinborn,  Vera Rebmann, Alexander Scharf, Christof Sohn, Hans Grosse-Wilde,, Soluble HLA-DR levels in the maternal circulation of normal and pathologic pregnancy, Am J Obstet Gynecol (2003) February:  473-479<br/>
[11] Roberta Rizzo, Cristina E. Mapp, Loredana Melchiorri, Piero Maestrelli,  Annalisa Visentin,  Stefano Ferretti, Ilaria Bononi, Deborah Miotto,  Olavio R. Baricordi, Defective production of soluble HLA-G molecules by peripheral blood monocytes in patients with asthma, J Allergy Clin Immunol (2004) Vol. 115: 508-513<br/>
[12] Bishwajit Nag, Teresa Kendrick, Subhashini Arimilli, Sheue Ching T. Yu, Subramaniam Sriram, Soluble MHC II–Peptide Complexes Induce Antigen-Specific Apoptosis in T Cells, Cellular Immunology (1996) Vol. 170: 25 –33<br/>
[13] Webb BJ, Bochan MR, Montel A, Padilla LM, Brahmi Z., The lack of NK cytotoxicity associated with fresh HUCB may be due to the presence of soluble HLA in the serum, Cellular Immunology (1994) Vol. 159: 246-261<br/>
[14]Sofia Casares, Cong S. Zong, Dorel L. Radu, Alexander Miller, Constantin A. Bona, and Teodor-Doru Brumeanu, Antigen-specific Signaling by a Soluble, Dimeric Peptide/Major Histocompatibility Complex Class II/Fc Chimera Leading to T Helper Cell Type 2 Differentiation J. Exp. Med. (1999) Vol. 190: 543–553<br/>
[15]Abdel Rahim A. Hamad, Sean M. O’Herrin, Michael S. Lebowitz, Ananth Srikrishnan, Joan Bieler, Jonathan Schneck, Drew Pardoll Potent T Cell Activation with Dimeric Peptide–Major Histocompatibility Complex Class II Ligand: The Role of CD4 Coreceptor, J. Exp. Med. (1998) Vol. 188: 1633–1640<br/>




UNDER CONSTRUCTION
===Events/News===


===Events/News===
===Past Great Events===
[[Image:Bandeau.jpg]]<br/>
[[Image:Bandeau.jpg]]<br/>
[http://www.eci-paris2006.com/'''1st Joint Meeting of ''European National Societies of Immunology'' 6-9 September, 2006, Paris''']<br/>
[http://www.eci-paris2006.com/'''1st Joint Meeting of ''European National Societies of Immunology'' 6-9 September, 2006, Paris''']<br/>
 
[http://www.biology.uoc.gr/postgraduate/summerschool/aims.html'''Summer school in Protein Biotechnology, 23-26 May 2007, Fodele Crete, Greece''']<br/>
===Top Stories===
[http://www.virologyj.com/content/3/1/60/abstract]No vaccine against HIV yet-are we not perfectly equipped?<br/>
[http://news.biocompare.com/newsstory.asp?id=148299]Scientists Stop Autoimmune Disease Without Shutting Off Immune System, 8/22/2006<br/>
[http://news.biocompare.com/newsstory.asp?id=148133]Montreal Researchers Make A Major Strategic Breakthrough In Controling The AIDS Virus, 8/21/2006<br/>


===Journals===
===Journals===
====General====
====General====
[http://www.nature.com/'''Nature Publishing Group''']<br/>
[http://www.nature.com/'''Nature Publishing Group''']<br/>
[http://www.cell.com/'''Cell''']<br/>
[http://www.sciencemag.org/'''Science''']<br/>
[http://www.annualreviews.org/'''Annual Reviews''']<br/>
[http://www.annualreviews.org/'''Annual Reviews''']<br/>
[http://www.newscientist.com/'''New Scientist''']
[http://www.newscientist.com/'''New Scientist''']<br/>
====Immunology====
 
[http://www.nature.com/ni/'''Nature Immunology''']<br/>
[http://www.nature.com/nri/'''Nature Reviews Immunology''']<br/>
[http://arjournals.annualreviews.org/loi/immunol?cookieSet=1/'''Annual Reviews of Immunology''']<br/>
[http://www.jimmunol.org/'''Journal of Immunology''']<br/>
[http://authors.elsevier.com/JournalDetail.html?PubID=622804&Precis/'''Cellular Immunology''']<br/>
[http://www.elsevier.com/locate/jim/'''Journal of Immunological Methods''']<br/>
[http://www.elsevier.de/imbio/'''Immunobiology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/622945/description#description/'''Seminars in Immunology''']<br/>
[http://www.elsevier.com/locate/humimm/'''Human Immunology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/405914/description#description/'''Trends in Immunology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/601305/description#description/'''Current Opinion in Immunology''']<br/>
[http://www3.interscience.wiley.com/cgi-bin/jhome/25061?CRETRY=1&SRETRY=0/'''European Journal of Immunology''']<br/>
[http://www.immunity.com/'''Immunity''']
====Open Access====
====Open Access====
=====PLoS=====
=====PLoS=====
[http://www.plos.org/'''PLoS''']<br/>
[http://www.plos.org/'''PLoS''']<br/>
[http://biology.plosjournals.org/perlserv/?request=index-html&issn=1545-7885/'''PLoS Biology''']<br/>
[*[http://www.biomedcentral.com/browse/journals/''' BMC journals here!!!''']
[http://medicine.plosjournals.org/perlserv/?request=index-html&issn=1549-1676/'''PLoS Medicine''']<br/>
[http://compbiol.plosjournals.org/perlserv/?request=index-html&issn=1553-7358/'''PLoS Computational Biology''']<br/>
[http://genetics.plosjournals.org/perlserv/?request=index-html&issn=1553-7404/'''PLoS Genetics''']<br/>
[http://pathogens.plosjournals.org/perlserv/?request=index-html&issn=1553-7374/'''PLoS Pathogens''']<br/>
[http://clinicaltrials.plosjournals.org/perlserv/?request=index-html&issn=1555-5887/'''PLoS Clinical Trials''']<br/>
[http://www.plosone.org/'''PLoS ONE''']
 
=====BMC=====
[http://www.biomedcentral.com/bmcbiol/'''BMC Biology''']<br/>
[http://www.biomedcentral.com/bmcbiotechnol/'''BMC Biotechnology''']<br/>
[http://www.biomedcentral.com/bmccancer/'''BMC Cancer''']<br/>
[http://www.biomedcentral.com/bmccellbiol/'''BMC Cell Biology''']<br/>
[http://www.biomedcentral.com/bmcgenet/'''BMC Genetics''']<br/>
[http://www.biomedcentral.com/bmcimmunol/'''BMC Immunology''']<br/>
[http://www.biomedcentral.com/bmcinfectdis/'''BMC Infectious Diseases''']<br/>
[http://www.biomedcentral.com/bmcmed/'''BMC Medicine''']<br/>
[http://www.biomedcentral.com/bmcmicrobiol/'''BMC Microbiology''']<br/>
*[http://www.biomedcentral.com/browse/journals/'''Many more BMC journals here!!!''']


===Links===
===Links===
[http://www.uoc.gr/'''University of Crete''']<br/>
[http://www.uoc.gr/'''University of Crete''']<br/>
[http://www.biology.uoc.gr/'''Department of Biology'''] Department of Biology, University of Crete, Greece<br/>
[http://www.biology.uoc.gr/'''Department of Biology'''] Department of Biology, University of Crete, Greece<br/>
[http://www.biology.uoc.gr/postgraduate/Probiotech/en/perigrafi/programa.html/'''M.Sc. Protein Biotechnology, University of Crete'''] M.Sc. Protein Biotechnology of the Department of Biology, University of Crete, Home Page<br/>
[http://www.cardiff.ac.uk/index.html/'''University of Cardiff'''] University of Cardiff, Wales, U.K.<br/>
[http://www.cardiff.ac.uk/index.html/'''University of Cardiff'''] University of Cardiff, Wales, U.K.<br/>
[http://www.pubmed.com/'''PubMed''']Major source of Bibliography and useful tools
[http://www3.newport.ac.uk/'''University of Newport'''] University of Newport, Wales, U.K.<br/>
[http://www.biocompare.com/'''Biocompare''']Guide for life sciences products<br/>
[http://www.imbb.forth.gr/people/aeconomou/teaching502.html'''BIO 502''']Bio 502 Economou & Tokatlidis<br/>
[http://pubs.usgs.gov/gip/microbes/'''How to collect and see bacteria''']<br/>
[http://www.biochem.ucl.ac.uk/bsm/sidechains/'''Atlas of Protein Side-Chain Interactions''']<br/>
[http://www.ruf.rice.edu/~bioslabs/studies/studies.htm'''This introduction to experimental biosciences will introduce and reinforce practical skills in the context of three different laboratory investigations..''']<br/>
[http://www.ruf.rice.edu/~bioslabs/schedules/Talks/Talk3.htm'''Superb presentation explaining how PAGE works''']<br/>
[http://ecoli.bham.ac.uk/'''The Escherichia coli database''']<br/>
[http://www.textbookofbacteriology.net/'''Todar's Online Textbook of Bacteriology''']<br/>
[http://www.bact.wisc.edu/themicrobialworld/homepage.html'''Ken Todar's Microbial World''']<br/>
[http://www.nottingham.ac.uk/quorum/index.htm'''Quorum sensing-University of Nottingham''']<br/>
[http://ec.europa.eu/research/rtdinfo/50/article_4230_en.html'''Fotis Kafatos: the model mentor''']<br/>
[http://www.ch.ic.ac.uk/vchemlib/course/mo_theory/main.html'''Introduction to Molecular Orbital Theory''']<br/>
[http://antoine.frostburg.edu/chem/senese/101/liquids/faq/h-bonding-vs-london-forces.shtml'''What are van der Waals forces?''']<br/>
 
===Internet tools===
[http://www.pubmed.com/'''PubMed-Major source of Bibliography and useful tools''']<br/>
[http://www.changbioscience.com/calculator/sdspc2.htm'''PAGE (SDS/Native) calculator''']<br/>
[http://www.graphpad.com/quickcalcs/Molarityform.cfm'''Molarity calculator''']<br/>
[http://www.lipidlibrary.co.uk'''The Lipid Library''']<br/>
[http://www.bioinfo.sfc.keio.ac.jp/class/bfg/PPI_Uetz/yeast.html'''Protein interactions in Yeast''']<br/>
[http://www.biotech.ou.edu/'''Recombinant Protein Solubility Prediction''']<br/>
[http://www.bmb.uga.edu/8010/moremen/weblinks/WebSerineProteases/SerProt.html'''Serine proteases mechanism''']<br/>
 
===Proteomics===
[http://www.expasy.org'''ExPASy Proteomics Server ''']<br/>
[http://www.expasy.org/swissmod/SWISS-MODEL.html'''SWISS-MODEL is a fully automated protein structure homology-modeling server, accessible via the ExPASy web server, or from the program DeepView''']<br/>
[http://www.ebi.ac.uk/clustalw/'''EBI Tools: ClustalW''']<br/>
[''' ''']<br/>
[''' ''']<br/>
 
===Interests===
[http://www.ebay.co.uk'''eBay UK''']The best place for shopping!!!<br/>
[http://www.paypal.com'''Paypal''']The best way to pay what you buy!!!<br/>
[http://www.myspace.com/mfsar'''Myspace Profile''']<br/>
[http://richarddawkins.net/'''Richard Dawkins net''']<br/>
[http://richarddawkinsfoundation.org/'''Richard Dawkins foundation''']
 
===Visits===
 
<html>
<a href="http://www2.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/User:Hartigan" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/User:Hartigan" style="border:1px solid;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onError="this.onError=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com'" />
</a></html>
 
<html>
<!-- Begin Shinystat Free code -->
<script type="text/javascript" language="JavaScript" SRC="http://codice.shinystat.com/cgi-bin/getcod.cgi?USER=hartigan"></script>
<noscript>
<A HREF="http://www.shinystat.com" target="_top">
<IMG SRC="http://www.shinystat.com/cgi-bin/shinystat.cgi?USER=hartigan" ALT="Advertising ROI" BORDER="0"></A>
</noscript>
<!-- Begin Shinystat Free code -->
 
 
 
 
 
<script src="http://www.google-analytics.com/urchin.js" type="text/javascript">
</script>
<script type="text/javascript">
_uacct = "UA-2935574-1";
urchinTracker();
</script>

Latest revision as of 04:24, 3 November 2007

current status:Studying, MS Protein Biotechnology

Sardis Marios Frantzeskos
Department of Biology
University of Crete
Herakleion, Crete
Greece

hartigan.sc@gmail.com

Education


2006-........MS in Protein Biotechnology, Department of Biology,University of Crete
2002-2006 BS in Biology (Biomolecular science and Biotechnology), University of Crete.
2001-2002 Certificate of Higher Education (in Biology), University of Cardiff, Wales, U.K.


Research experience

Past research interests

MHC class II molecules

Current research interests

Molecular motors Protein translocation mechanisms in E.coli through the SecA-SecYEG system-Institute of Molecular Biology and Biotechnology-Foundation of Research and Technology Hellas (FORTH), Herakleion, Greece
SecA translocase
Ecolab


Events/News

Past Great Events


1st Joint Meeting of European National Societies of Immunology 6-9 September, 2006, Paris
Summer school in Protein Biotechnology, 23-26 May 2007, Fodele Crete, Greece

Journals

General

Nature Publishing Group
Cell
Science
Annual Reviews
New Scientist

Open Access

PLoS

PLoS
[* BMC journals here!!!

Links

University of Crete
Department of Biology Department of Biology, University of Crete, Greece
M.Sc. Protein Biotechnology, University of Crete M.Sc. Protein Biotechnology of the Department of Biology, University of Crete, Home Page
University of Cardiff University of Cardiff, Wales, U.K.
University of Newport University of Newport, Wales, U.K.
BiocompareGuide for life sciences products
BIO 502Bio 502 Economou & Tokatlidis
How to collect and see bacteria
Atlas of Protein Side-Chain Interactions
This introduction to experimental biosciences will introduce and reinforce practical skills in the context of three different laboratory investigations..
Superb presentation explaining how PAGE works
The Escherichia coli database
Todar's Online Textbook of Bacteriology
Ken Todar's Microbial World
Quorum sensing-University of Nottingham
Fotis Kafatos: the model mentor
Introduction to Molecular Orbital Theory
What are van der Waals forces?

Internet tools

PubMed-Major source of Bibliography and useful tools
PAGE (SDS/Native) calculator
Molarity calculator
The Lipid Library
Protein interactions in Yeast
Recombinant Protein Solubility Prediction
Serine proteases mechanism

Proteomics

ExPASy Proteomics Server
SWISS-MODEL is a fully automated protein structure homology-modeling server, accessible via the ExPASy web server, or from the program DeepView
EBI Tools: ClustalW
[ ]
[ ]

Interests

eBay UKThe best place for shopping!!!
PaypalThe best way to pay what you buy!!!
Myspace Profile
Richard Dawkins net
Richard Dawkins foundation

Visits

<html> <a href="http://www2.clustrmaps.com/counter/maps.php?url=http://openwetware.org/wiki/User:Hartigan" id="clustrMapsLink"><img src="http://www2.clustrmaps.com/counter/index2.php?url=http://openwetware.org/wiki/User:Hartigan" style="border:1px solid;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onError="this.onError=null; this.src='http://clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://clustrmaps.com'" /> </a></html>

<html> <script type="text/javascript" language="JavaScript" SRC="http://codice.shinystat.com/cgi-bin/getcod.cgi?USER=hartigan"></script> <noscript> <A HREF="http://www.shinystat.com" target="_top"> <IMG SRC="http://www.shinystat.com/cgi-bin/shinystat.cgi?USER=hartigan" ALT="Advertising ROI" BORDER="0"></A> </noscript>



<script src="http://www.google-analytics.com/urchin.js" type="text/javascript"> </script> <script type="text/javascript"> _uacct = "UA-2935574-1"; urchinTracker(); </script>

Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Marios_F._Sardis&oldid=164075"