User:Marios F. Sardis: Difference between revisions

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====RESHAPING THE PAGE====
====Research experience====


*Sardis MF, Athanassakis I., Modulation of spleen cell responces by soluble MHC class II molecules, 1st Joint Meeting of European National Societies of Immunology, Book of Abstracts (Poster presentation), 6 September 2006<br/>
==Past research interests==


===Research experience===
[[MHC class II molecules]]
[[Image:Dark_field_all.jpg|thumb|100px|right|Dark field microscopy of spleen cells]]


== MHC class II molecules ==
==Current research interests==


 
Molecular motors
In 1967, a puzzling observation was made by Calne and his laboratory team, during liver transplantation experiments on pigs. One animal survived for months, after receiving an allograft, without any immunosupression. Later, it was found that the soluble factors, which were secreted by the liver allograft and were responsible for the development of tolerance to the recipient animal, were soluble MHC (sMHC) molecules.From this point, many laboratories around the world entered the quest, for the elucidation of the immunoregulatory effects of the various soluble forms of MHC molecules. Today, it is widely accepted that body fluids, isolated from healthy individuals contain various amounts of soluble MHC molecules. The origin of these molecules has not been clearly determined and it could be the result of shedding, alternative splicing or active secretion, whereas it has been demonstrated that trophoblast cells secrete to the medium soluble HLA-DR molecules when stimulated by IFNγ. In the 70s and 80s during the study of T suppressors (some of which might correspond to the modern T regulatory cells), it was suggested that they affected nearby cells secreting various factors with MHC determinants. [[Image:Isolation.jpg|thumb|200px|left|Sceme of the isolation strategy we followed]]
Protein translocation mechanisms in E.coli through the SecA-SecYEG system-Institute of Molecular Biology and Biotechnology-Foundation of Research and Technology Hellas (FORTH), Herakleion, Greece <br/>
Deviations from the concentration of these molecules in the body fluids of healthy individuals, have been recorder in numerous pathological conditions, such as viral encephalitis, rheumatoid arthritis, pathologic pregnancies, asthma and AIDS. These results imply that soluble MHC molecules have a special role, in the pathology of these diseases but it is not clarified whether they contribute to the pathology or if they are by-products of the pathological condition (they could have a potential role in the diagnosis of the disease stage). Additional research has confirmed that soluble forms of MHC molecules can have various immunomodulating effects, such as induction of apoptosis of CD4+ activation of CD8+ T cells and down-regulation of NK activity.
The aim of this study was to perform a downstream investigation of the possible immunomodulating action of soluble MHC molecules isolated from the serum of male and pregnant Balb/c mice. We selected the 12th day of gestation for the isolation of the serum, according to Ranella A. (The role and the regulatory mechanisms of growth factors, membrane and secreted MHC class II molecules in the survival of the hemiallogeneic embryo during gestation, Ph.D. thesis, University of Crete, unpublished data), because the titer of the serum reaches a maximum. During the study we used affinity chromatography and immunoprecipitation for the isolation of sMHC II from the serum and cell culture techniques, in order to reveal any effect on freshly isolated spleen cells. Pregnancy is often called immune paradox, because the immune system of the mother allows to the fetus to survive although it is an allotransplant. Generally, pregnancy is largely considered to be a TH2 phenomenon that is characterized by downregulation of the immune system in the point where the mothers body meets the fetus. Soluble MHC class II molecules secreted by trophoblast cells could contribute to the development of tolerance, because the ability of antigen presentation from the trophoblast is lost along with the secreted MHC II. However, it has not been determined whether the molecules we isolate from the serum originate from the trophoblast. The presence of the same molecules in the serum of male mice suggests an alternative source. Our results revealed the following information:
*SDS-PAGE assays revealed a prominent band at about 67kDa.<br\>
*Upon the addition of these molecules, spleen cells from syngeneic mice respond positively and upregulate their activation status.<br\>
*In Mixed Lymphocyte Reactions, which depend on the successful activation of CD4+ T helpers, the incorporation of radioactivity was boosted when sMHC II (from pregnant) were added to Balb/c spleen cells, which responded to inactivated with mitomycin C C3H/HeN spleen cells, CD4 receptor seems to be important for the effect we recorded, since the incorporation of radioactivity diminishes when we interfere with it.<br\>
*An important decrease in the level of IgM has also been recorded when sMHC from pregnant and male mice were added to the medium. This could result from interference of cell-cell interactions or by favoring the expression of Type-1 cytokine profile.<br\>
*Type-1 cytokines were increased in the samples that were treated with sMHC II from male mice.<br\>
*Type-2 cytokines were increased in the samples that were treated with sMHC II from pregnant mice.<br\>
*Our soluble molecules have the potential to cause robust activation of spleen cells, which is comparable with the activation caused by up to 100000cfu of Legionella pneumophila ser.1<br\>
*When spleen cells were challenged with L.pneumophila fragments the addition of sMHC II from male mice produced a Type-1 cytokine profile, whereas the addition of sMHC II from female mice a Type-2 profile (data notpresented). However, both molecules seemed to upregulate the production of IL-15.<br\>
*Soluble molecules isolated from female pregnant Balb/c mice might have the potential to downregulate the proliferation of syngeneic placenta cells (preliminary data, not presented).
 
== Molecular motors ==
SecA mutants in E.coli-Rotation in Institute of Molecular Biology and Biotechnology-Foundation of Research and Technology Hellas (FORTH), Herakleion, Greece <br/>
[http://www.imbb.forth.gr/people/aeconomou/sec.html'''SecA translocase''']<br/>
[http://www.imbb.forth.gr/people/aeconomou/sec.html'''SecA translocase''']<br/>
[http://www.imbb.forth.gr/people/aeconomou/'''Ecolab''']<br/>
[http://www.imbb.forth.gr/people/aeconomou/'''Ecolab''']<br/>
If you have any questions or anything you would like to discuss with me (need any sources, articles, reviews etc....) about all these do not hesitate to contact me at '''''hartigan.sc@gmail.com'''''




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[[Image:Bandeau.jpg]]<br/>
[[Image:Bandeau.jpg]]<br/>
[http://www.eci-paris2006.com/'''1st Joint Meeting of ''European National Societies of Immunology'' 6-9 September, 2006, Paris''']<br/>
[http://www.eci-paris2006.com/'''1st Joint Meeting of ''European National Societies of Immunology'' 6-9 September, 2006, Paris''']<br/>
[[Image:Eifel.jpg|300px]][[Image:Me_paris.jpg|300px]]
[http://www.biology.uoc.gr/postgraduate/summerschool/aims.html'''Summer school in Protein Biotechnology, 23-26 May 2007, Fodele Crete, Greece''']<br/>
 
===Top Stories===
[http://www.biomedcentral.com/content/pdf/1741-7007-4-7.pdf'''Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25''']<br/>
[http://www.biomedcentral.com/1471-2407/6/233/abstract'''High-resolution analysis of HLA class I alterations in colorectal cancer''']<br/>
[http://www.virologyj.com/content/3/1/60/abstract'''No vaccine against HIV yet-are we not perfectly equipped?''']<br/>
[http://news.biocompare.com/newsstory.asp?id=148299'''Scientists Stop Autoimmune Disease Without Shutting Off Immune System''']<br/>
[http://news.biocompare.com/newsstory.asp?id=148133'''Montreal Researchers Make A Major Strategic Breakthrough In Controling The AIDS Virus''']<br/>


===Journals===
===Journals===
====General====
====General====
[http://www.nature.com/'''Nature Publishing Group''']<br/>
[http://www.nature.com/'''Nature Publishing Group''']<br/>
[http://www.cell.com/'''Cell''']<br/>
[http://www.sciencemag.org/'''Science''']<br/>
[http://www.annualreviews.org/'''Annual Reviews''']<br/>
[http://www.annualreviews.org/'''Annual Reviews''']<br/>
[http://www.newscientist.com/'''New Scientist''']
[http://www.newscientist.com/'''New Scientist''']<br/>
====Immunology====
 
[http://www.nature.com/ni/'''Nature Immunology''']<br/>
[http://www.nature.com/nri/'''Nature Reviews Immunology''']<br/>
[http://arjournals.annualreviews.org/loi/immunol?cookieSet=1/'''Annual Reviews of Immunology''']<br/>
[http://www.jimmunol.org/'''Journal of Immunology''']<br/>
[http://authors.elsevier.com/JournalDetail.html?PubID=622804&Precis/'''Cellular Immunology''']<br/>
[http://www.elsevier.com/locate/jim/'''Journal of Immunological Methods''']<br/>
[http://www.elsevier.de/imbio/'''Immunobiology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/622945/description#description/'''Seminars in Immunology''']<br/>
[http://www.elsevier.com/locate/humimm/'''Human Immunology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/405914/description#description/'''Trends in Immunology''']<br/>
[http://www.elsevier.com/wps/find/journaldescription.cws_home/601305/description#description/'''Current Opinion in Immunology''']<br/>
[http://www3.interscience.wiley.com/cgi-bin/jhome/25061?CRETRY=1&SRETRY=0/'''European Journal of Immunology''']<br/>
[http://www.immunity.com/'''Immunity''']
====Open Access====
====Open Access====
=====PLoS=====
=====PLoS=====
[http://www.plos.org/'''PLoS''']<br/>
[http://www.plos.org/'''PLoS''']<br/>
[http://biology.plosjournals.org/perlserv/?request=index-html&issn=1545-7885/'''PLoS Biology''']<br/>
[*[http://www.biomedcentral.com/browse/journals/''' BMC journals here!!!''']
[http://medicine.plosjournals.org/perlserv/?request=index-html&issn=1549-1676/'''PLoS Medicine''']<br/>
[http://compbiol.plosjournals.org/perlserv/?request=index-html&issn=1553-7358/'''PLoS Computational Biology''']<br/>
[http://genetics.plosjournals.org/perlserv/?request=index-html&issn=1553-7404/'''PLoS Genetics''']<br/>
[http://pathogens.plosjournals.org/perlserv/?request=index-html&issn=1553-7374/'''PLoS Pathogens''']<br/>
[http://clinicaltrials.plosjournals.org/perlserv/?request=index-html&issn=1555-5887/'''PLoS Clinical Trials''']<br/>
[http://www.plosone.org/'''PLoS ONE''']
 
=====BMC=====
[http://www.biomedcentral.com/bmcbiol/'''BMC Biology''']<br/>
[http://www.biomedcentral.com/bmcbiotechnol/'''BMC Biotechnology''']<br/>
[http://www.biomedcentral.com/bmccancer/'''BMC Cancer''']<br/>
[http://www.biomedcentral.com/bmccellbiol/'''BMC Cell Biology''']<br/>
[http://www.biomedcentral.com/bmcgenet/'''BMC Genetics''']<br/>
[http://www.biomedcentral.com/bmcimmunol/'''BMC Immunology''']<br/>
[http://www.biomedcentral.com/bmcinfectdis/'''BMC Infectious Diseases''']<br/>
[http://www.biomedcentral.com/bmcmed/'''BMC Medicine''']<br/>
[http://www.biomedcentral.com/bmcmicrobiol/'''BMC Microbiology''']<br/>
*[http://www.biomedcentral.com/browse/journals/'''Many more BMC journals here!!!''']


===Links===
===Links===
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[http://www3.newport.ac.uk/'''University of Newport'''] University of Newport, Wales, U.K.<br/>
[http://www3.newport.ac.uk/'''University of Newport'''] University of Newport, Wales, U.K.<br/>
[http://www.biocompare.com/'''Biocompare''']Guide for life sciences products<br/>
[http://www.biocompare.com/'''Biocompare''']Guide for life sciences products<br/>
[http://www.imbb.forth.gr/people/aeconomou/teaching502.html'''BIO 502''']Bio 502 Economou & Tokatlidis
[http://www.imbb.forth.gr/people/aeconomou/teaching502.html'''BIO 502''']Bio 502 Economou & Tokatlidis<br/>
[http://pubs.usgs.gov/gip/microbes/'''How to collect and see bacteria''']<br/>
[http://www.biochem.ucl.ac.uk/bsm/sidechains/'''Atlas of Protein Side-Chain Interactions''']<br/>
[http://www.ruf.rice.edu/~bioslabs/studies/studies.htm'''This introduction to experimental biosciences will introduce and reinforce practical skills in the context of three different laboratory investigations..''']<br/>
[http://www.ruf.rice.edu/~bioslabs/schedules/Talks/Talk3.htm'''Superb presentation explaining how PAGE works''']<br/>
[http://ecoli.bham.ac.uk/'''The Escherichia coli database''']<br/>
[http://www.textbookofbacteriology.net/'''Todar's Online Textbook of Bacteriology''']<br/>
[http://www.bact.wisc.edu/themicrobialworld/homepage.html'''Ken Todar's Microbial World''']<br/>
[http://www.nottingham.ac.uk/quorum/index.htm'''Quorum sensing-University of Nottingham''']<br/>
[http://ec.europa.eu/research/rtdinfo/50/article_4230_en.html'''Fotis Kafatos: the model mentor''']<br/>
[http://www.ch.ic.ac.uk/vchemlib/course/mo_theory/main.html'''Introduction to Molecular Orbital Theory''']<br/>
[http://antoine.frostburg.edu/chem/senese/101/liquids/faq/h-bonding-vs-london-forces.shtml'''What are van der Waals forces?''']<br/>


===Internet tools===
===Internet tools===
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[http://www.lipidlibrary.co.uk'''The Lipid Library''']<br/>
[http://www.lipidlibrary.co.uk'''The Lipid Library''']<br/>
[http://www.bioinfo.sfc.keio.ac.jp/class/bfg/PPI_Uetz/yeast.html'''Protein interactions in Yeast''']<br/>
[http://www.bioinfo.sfc.keio.ac.jp/class/bfg/PPI_Uetz/yeast.html'''Protein interactions in Yeast''']<br/>
[http://www.biotech.ou.edu/'''Recombinant Protein Solubility Prediction''']<br/>
[http://www.bmb.uga.edu/8010/moremen/weblinks/WebSerineProteases/SerProt.html'''Serine proteases mechanism''']<br/>


===Proteomics===
===Proteomics===
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[http://www.ebay.co.uk'''eBay UK''']The best place for shopping!!!<br/>
[http://www.ebay.co.uk'''eBay UK''']The best place for shopping!!!<br/>
[http://www.paypal.com'''Paypal''']The best way to pay what you buy!!!<br/>
[http://www.paypal.com'''Paypal''']The best way to pay what you buy!!!<br/>
[http://www.myspace.com/mfsar'''Myspace Profile''']Still not completed
[http://www.myspace.com/mfsar'''Myspace Profile''']<br/>
[http://richarddawkins.net/'''Richard Dawkins net''']<br/>
[http://richarddawkinsfoundation.org/'''Richard Dawkins foundation''']


===Visits===
===Visits===
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Latest revision as of 04:24, 3 November 2007

current status:Studying, MS Protein Biotechnology

Sardis Marios Frantzeskos
Department of Biology
University of Crete
Herakleion, Crete
Greece

hartigan.sc@gmail.com

Education


2006-........MS in Protein Biotechnology, Department of Biology,University of Crete
2002-2006 BS in Biology (Biomolecular science and Biotechnology), University of Crete.
2001-2002 Certificate of Higher Education (in Biology), University of Cardiff, Wales, U.K.


Research experience

Past research interests

MHC class II molecules

Current research interests

Molecular motors Protein translocation mechanisms in E.coli through the SecA-SecYEG system-Institute of Molecular Biology and Biotechnology-Foundation of Research and Technology Hellas (FORTH), Herakleion, Greece
SecA translocase
Ecolab


Events/News

Past Great Events


1st Joint Meeting of European National Societies of Immunology 6-9 September, 2006, Paris
Summer school in Protein Biotechnology, 23-26 May 2007, Fodele Crete, Greece

Journals

General

Nature Publishing Group
Cell
Science
Annual Reviews
New Scientist

Open Access

PLoS

PLoS
[* BMC journals here!!!

Links

University of Crete
Department of Biology Department of Biology, University of Crete, Greece
M.Sc. Protein Biotechnology, University of Crete M.Sc. Protein Biotechnology of the Department of Biology, University of Crete, Home Page
University of Cardiff University of Cardiff, Wales, U.K.
University of Newport University of Newport, Wales, U.K.
BiocompareGuide for life sciences products
BIO 502Bio 502 Economou & Tokatlidis
How to collect and see bacteria
Atlas of Protein Side-Chain Interactions
This introduction to experimental biosciences will introduce and reinforce practical skills in the context of three different laboratory investigations..
Superb presentation explaining how PAGE works
The Escherichia coli database
Todar's Online Textbook of Bacteriology
Ken Todar's Microbial World
Quorum sensing-University of Nottingham
Fotis Kafatos: the model mentor
Introduction to Molecular Orbital Theory
What are van der Waals forces?

Internet tools

PubMed-Major source of Bibliography and useful tools
PAGE (SDS/Native) calculator
Molarity calculator
The Lipid Library
Protein interactions in Yeast
Recombinant Protein Solubility Prediction
Serine proteases mechanism

Proteomics

ExPASy Proteomics Server
SWISS-MODEL is a fully automated protein structure homology-modeling server, accessible via the ExPASy web server, or from the program DeepView
EBI Tools: ClustalW
[ ]
[ ]

Interests

eBay UKThe best place for shopping!!!
PaypalThe best way to pay what you buy!!!
Myspace Profile
Richard Dawkins net
Richard Dawkins foundation

Visits

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