User:Marios F. Sardis/Protein Biotechnology: Difference between revisions
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[http://www.michaelsmith.ubc.ca/services/Calorimetry/'''University of British Columbia''']<br/> | [http://www.michaelsmith.ubc.ca/services/Calorimetry/'''University of British Columbia''']<br/> | ||
[http://www.york.ac.uk/depts/biol/tf/mi_itc.htm'''University of York''']<br/> | [http://www.york.ac.uk/depts/biol/tf/mi_itc.htm'''University of York''']<br/> | ||
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[http://www.maxanim.com/biochemistry/Electron%20Transport%20Chain%20and%20ATP%20Synthesis/Electron%20Transport%20Chain%20and%20ATP%20Synthesis.htm'''Oxidative Phosphorylation Movie''']<br/> | [http://www.maxanim.com/biochemistry/Electron%20Transport%20Chain%20and%20ATP%20Synthesis/Electron%20Transport%20Chain%20and%20ATP%20Synthesis.htm'''Oxidative Phosphorylation Movie''']<br/> | ||
BIO504 | ==BIO504== | ||
[[http://www.olympusfluoview.com/applications/fretintro.html'''Excellent tutorial for FRET by Olympus''']]<br/> | [[http://www.olympusfluoview.com/applications/fretintro.html'''Excellent tutorial for FRET by Olympus''']]<br/> | ||
Revision as of 06:22, 10 December 2006
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MSc/PhD Protein Biotechnology
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Protein Biotechnology
- A non official page about the postgraduate program of Protein Biotechnology, of the University of Crete maintained by students that participate in it.
THE PAGE IS UNDER CONSTRUCTION-IT WILL BE READY REALLY SOON
Protein Biotechnology, University of Crete, Greece
Program is valid for 2006-2007
Bio501 Protein Structure and function
Coordinators: Κ. Petratos
CONTENTS
- Elements of Protein Biochemistry
Ν. Μοschonas 3 hours
Architectural elements of the genome, types of DNA sequences, genes coding for proteins, multi-gene families, structure-function relationships of proteins
Μ. Κοkkinidis 6 hours
Structural and physical chemical properties of proteins
- Enzyme Catalysis
- Introduction to Enzymes
The chemical nature of enzymes and definitions of basic terms of enzymology
Structural characteristics of known enzymes
- Enzyme Kinetics
Basic Principles
The 'Michaelis-Menten' Theory
Definitions of kinetic parameters
Estimation of kinetic parameters via plotting of appropriate functions
Chemical kinetics of enzymatic reactions with multiple substrates
Enzyme inhibition
Analysis of the 'Hill' plots
Regulation of enzymatic function
- Mechanisms of Enzymatic Catalysis
General principles of enzymatic catalysis
- Molecular Basis of some Genetic Diseases
Contributions of the elucidation of the function of proteins
PROTEINS IN THE INTRA- AND EXTRACELLULAR ENVIRONMENT (BIO502)
Coordinators: A. Economou
CONTENTS INSTRUCTOR DURATION
Introduction in the structure and in the function of the Cell. Description of our friend, the
E. coli bacterium. Basics of DNA replication, transcription and translation.
General principles of protein translocation (targeting and transport) especially in bacteria.
Focus on Sec pathway, Type III and TAT translocators.
Energetic properties of membranes.
Chaperone function
[Tokatlidis K.]
General information about cell organization, membranes etc.
Trafficing of proteins:
From the cytoplasm to mitochondria,chloroplasts and peroxysomes
From the cytoplasm to the nucleus
Vesicular Transport
Protein Folding
Post-translational modification of proteins
Modification in the cytoplasm
Modification in the secretory pathway
Protein complexes and protein engines Membrane Transport Proteins:(carrier and channel proteins, e.g. F0F1ATPase,Na+/K+ ATPase, Ca2+ channel etc.) Protein engines involved in synthesis:(ribosome, transcription initiation complex, etc.) Multiprotein complexes in cell signaling.
Chalepakis
Cytoskeletal proteins and molecular motors.
The nexus between cytoskeleton
and extracellular space
The proteinous network of the extracellular matrix: (collagen, elastin etc.)
ADDITIONAL SEMINARS OF THE COURSE
I. Athanassaki
Proteins in the defense of organisms: The major histocompatibility complex, cytokines, chemokines, antigen presentation.
BIO503=
Circular Dichroism
Excellent demonstration of CD principles
Calorimetry
ITC &DSC
University of British Columbia
University of York
(under construction)
(UNDER CONSTRUCTION)
Oxidative Phosphorylation Movie
BIO504
[Excellent tutorial for FRET by Olympus]
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