# User:Mary Mendoza/Notebook/CHEM572 Exp. Biological Chemistry II/2013/01/23

(Difference between revisions)
 Revision as of 16:34, 23 January 2013 (view source) (→Calculation of reagents for ADA kinetic assay)← Previous diff Current revision (18:09, 8 May 2013) (view source) (→Calculation of reagents for ADA kinetic assay) Line 6: Line 6: | colspan="2"| | colspan="2"| - ==Calculation of reagents for ADA kinetic assay== - * It was decided to prepare a 30 mM stock solution of adenosine. The calculation below shows the amount needed to obtain 30 mM of adenosine. - - .030  $\frac{mol}{L}$ mol of adenosine × $\frac{267.24 g}{1 mol}$ = $\frac{8.0172 g}{L}$ × .010 L = 0.0802 g of adenosine - - - The calculations on the bottom are courtesy of [[User:Dhea Patel/Notebook/CHEM 572: ADA&Inhibitor Kinetics/2013/01/23|Dhea Patel]]. - - [[Image:ADA_kinetics_table.png|px80]] - - - The calculation of for the buffer was formulated by [[User:Catherine Koenigsknecht/Notebook/Experimental Biological Chemistry/2013/01/23|Catherine Koenigsknecht]]. - - * Procedure: Buffer - #Protocol for 0.1 M Sodium Phosphate Buffer (pH 7.4) - #Add 3.1 g of NaH2PO4•H2O and 10.9 g of Na2HPO4 (anhydrous) to distilled H2O to make a volume of 1 L. The pH of the final solution will be 7.4. This buffer can be stored for up to 1 mo at 4°C. - #Dilute to 0.05M: (take 50 mL of 1M solution and dilute in 1 Liter H2O)