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Preparation of ADA Kinetic Assay Reactants
- Adenosine deaminase was ordered from Worthington (19.8 u/mg). To obtain 0.5 M per mL, the calculation below was made amounting to 0.0253.
[math]\displaystyle{ \frac{0.5 units}{19.8 u/mg} }[/math] = 0.0253 mg
- For convenience, it was decided to dissolve 2.5 mg into 100 mL of phosphate buffer. The final amount weighed 2.0 mg which will be dissolved into 80 mL of phosphate buffer. ADA was refrigerated between 2-8°C temporarily.
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